Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it>
<p>Abstract</p> <p>Background</p> <p>Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. <it>Trichoderma </it>spp. are largely used in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-07-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/7/41 |
| _version_ | 1811287030455336960 |
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| author | Mariani Paola Woo Sheridan L Zuppini Anna Moscatiello Roberto Baldan Barbara Navazio Lorella Lorito Matteo |
| author_facet | Mariani Paola Woo Sheridan L Zuppini Anna Moscatiello Roberto Baldan Barbara Navazio Lorella Lorito Matteo |
| author_sort | Mariani Paola |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. <it>Trichoderma </it>spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown.</p> <p>Results</p> <p>Here, we investigated the effects on plant cells of metabolite complexes secreted by <it>Trichoderma atroviride </it>wild type P1 and a deletion mutant of this strain on the level of cytosolic free Ca<sup>2+ </sup>and activation of defense responses. <it>Trichoderma </it>culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen <it>Botrytis cinerea</it>, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (<it>Glycine max </it>L.) cell suspension cultures, <it>Trichoderma </it>and <it>Botrytis </it>metabolite mixtures were distinctively perceived and activated transient intracellular Ca<sup>2+ </sup>elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both Ca<sup>2+ </sup>signature and cellular effects were modified by the culture medium from the knock-out mutant of <it>Trichoderma</it>, defective for the production of the secreted 42 kDa endochitinase.</p> <p>Conclusion</p> <p>New insights are provided into the mechanism of interaction between <it>Trichoderma </it>and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular Ca<sup>2+ </sup>changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses.</p> |
| first_indexed | 2024-04-13T03:10:25Z |
| format | Article |
| id | doaj.art-fe0b9464ed9843bf872def5c192a0574 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T03:10:25Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-fe0b9464ed9843bf872def5c192a05742022-12-22T03:05:05ZengBMCBMC Plant Biology1471-22292007-07-01714110.1186/1471-2229-7-41Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it>Mariani PaolaWoo Sheridan LZuppini AnnaMoscatiello RobertoBaldan BarbaraNavazio LorellaLorito Matteo<p>Abstract</p> <p>Background</p> <p>Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. <it>Trichoderma </it>spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown.</p> <p>Results</p> <p>Here, we investigated the effects on plant cells of metabolite complexes secreted by <it>Trichoderma atroviride </it>wild type P1 and a deletion mutant of this strain on the level of cytosolic free Ca<sup>2+ </sup>and activation of defense responses. <it>Trichoderma </it>culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen <it>Botrytis cinerea</it>, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (<it>Glycine max </it>L.) cell suspension cultures, <it>Trichoderma </it>and <it>Botrytis </it>metabolite mixtures were distinctively perceived and activated transient intracellular Ca<sup>2+ </sup>elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both Ca<sup>2+ </sup>signature and cellular effects were modified by the culture medium from the knock-out mutant of <it>Trichoderma</it>, defective for the production of the secreted 42 kDa endochitinase.</p> <p>Conclusion</p> <p>New insights are provided into the mechanism of interaction between <it>Trichoderma </it>and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular Ca<sup>2+ </sup>changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses.</p>http://www.biomedcentral.com/1471-2229/7/41 |
| spellingShingle | Mariani Paola Woo Sheridan L Zuppini Anna Moscatiello Roberto Baldan Barbara Navazio Lorella Lorito Matteo Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> BMC Plant Biology |
| title | Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> |
| title_full | Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> |
| title_fullStr | Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> |
| title_full_unstemmed | Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> |
| title_short | Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus <it>Trichoderma atroviride</it> |
| title_sort | calcium mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus it trichoderma atroviride it |
| url | http://www.biomedcentral.com/1471-2229/7/41 |
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