Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF)
In this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analys...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-05-01
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| Online Access: | http://www.mdpi.com/2297-8739/5/2/27 |
| _version_ | 1798034220708265984 |
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| author | Torgny Undin Andreas P. Dahlin Jonas Bergquist Sara Bergström Lind |
| author_facet | Torgny Undin Andreas P. Dahlin Jonas Bergquist Sara Bergström Lind |
| author_sort | Torgny Undin |
| collection | DOAJ |
| description | In this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was employed for the analysis of proteins in ventricular cerebrospinal fluid (vCSF) from neurointensive care patients. Protein adsorption was studied by in-situ sampling in a temporally resolved manner on both immobilized native and Pluronic-deactivated membranes. Deactivation was significantly reducing the protein adsorption but it also induced novel selective properties of the surface. The proposed versatile strategy will facilitate protein-biomaterial, protein-polymer, protein-protein interaction studies in the future. |
| first_indexed | 2024-04-11T20:41:18Z |
| format | Article |
| id | doaj.art-fe0f91f6cf934f66a879cce1696a29dd |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-11T20:41:18Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-fe0f91f6cf934f66a879cce1696a29dd2022-12-22T04:04:13ZengMDPI AGSeparations2297-87392018-05-01522710.3390/separations5020027separations5020027Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF)Torgny Undin0Andreas P. Dahlin1Jonas Bergquist2Sara Bergström Lind3Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, SwedenDepartment of Engineering Sciences, Uppsala University, PO Box 534, SE-751 21 Uppsala, SwedenDepartment of Chemistry-BMC, Analytical Chemistry, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, SwedenDepartment of Chemistry-BMC, Analytical Chemistry, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, SwedenIn this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was employed for the analysis of proteins in ventricular cerebrospinal fluid (vCSF) from neurointensive care patients. Protein adsorption was studied by in-situ sampling in a temporally resolved manner on both immobilized native and Pluronic-deactivated membranes. Deactivation was significantly reducing the protein adsorption but it also induced novel selective properties of the surface. The proposed versatile strategy will facilitate protein-biomaterial, protein-polymer, protein-protein interaction studies in the future.http://www.mdpi.com/2297-8739/5/2/27protein adsorptionsurface modificationPluronic F-127LC MS/MS |
| spellingShingle | Torgny Undin Andreas P. Dahlin Jonas Bergquist Sara Bergström Lind Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) Separations protein adsorption surface modification Pluronic F-127 LC MS/MS |
| title | Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) |
| title_full | Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) |
| title_fullStr | Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) |
| title_full_unstemmed | Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) |
| title_short | Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF) |
| title_sort | mass spectrometric determination of the effect of surface deactivation on membranes used for in situ sampling of cerebrospinal fluid csf |
| topic | protein adsorption surface modification Pluronic F-127 LC MS/MS |
| url | http://www.mdpi.com/2297-8739/5/2/27 |
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