Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice
Abstract In the present study, Ag/AgCl‐NPs were biosynthesised using Hypnea musciformis seaweed extract; NPs synthesis was confirmed by a change of colour and observation of a razor‐sharp peak at 424 nm by UV–visible spectroscopy. Synthesised nanoparticles were characterised by transmission electron...
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| Format: | Article |
| Language: | English |
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Hindawi-IET
2022-04-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12075 |
| _version_ | 1797431392023347200 |
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| author | Rita Ghose A. K. M. Asaduzzaman Imtiaj Hasan Syed Rashel Kabir |
| author_facet | Rita Ghose A. K. M. Asaduzzaman Imtiaj Hasan Syed Rashel Kabir |
| author_sort | Rita Ghose |
| collection | DOAJ |
| description | Abstract In the present study, Ag/AgCl‐NPs were biosynthesised using Hypnea musciformis seaweed extract; NPs synthesis was confirmed by a change of colour and observation of a razor‐sharp peak at 424 nm by UV–visible spectroscopy. Synthesised nanoparticles were characterised by transmission electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray powder diffraction and Fourier transform infrared spectroscopy. Bacterial cell growth inhibition proves that the Ag/AgCl‐NPs have strong antibacterial activity and cell morphological alteration was observed in treated bacterial cells using propidium iodide (PI). Ag/AgCl‐NPs inhibited Ehrlich ascites carcinoma (EAC) cells, colorectal cancer (HCT‐116) and breast cancer (MCF‐7) cell line in vitro with the IC50 values of 40.45, 24.08 and 36.95 μg/ml, respectively. Initiation of apoptosis in HCT‐116 and MCF‐7 cells was confirmed using PI, FITC‐annexin V and Hoechst 33342 dye. No reaction oxygen species generation was observed in both treated and untreated cell lines. A significant increase of ATG‐5 gene expression indicates the possibility of autophagy cell death besides apoptosis in MCF‐7 cells. The initiation of apoptosis in EAC cells was confirmed by observing caspase‐3 protein expression. Ag/AgCl‐NPs inhibited 22.83% and 51% of the EAC cell growth in vivo in mice when administered 1.5 and 3.0 mg/kg/day (i.p.), respectively, for 5 consequent days. |
| first_indexed | 2024-03-09T09:44:17Z |
| format | Article |
| id | doaj.art-fe139f29c7e3460aa6ace16e1eb61b08 |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2024-03-09T09:44:17Z |
| publishDate | 2022-04-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-fe139f29c7e3460aa6ace16e1eb61b082023-12-02T00:43:35ZengHindawi-IETIET Nanobiotechnology1751-87411751-875X2022-04-01162496010.1049/nbt2.12075Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in miceRita Ghose0A. K. M. Asaduzzaman1Imtiaj Hasan2Syed Rashel Kabir3Department of Biochemistry and Molecular Biology Faculty of Science University of Rajshahi Rajshahi BangladeshDepartment of Biochemistry and Molecular Biology Faculty of Science University of Rajshahi Rajshahi BangladeshDepartment of Biochemistry and Molecular Biology Faculty of Science University of Rajshahi Rajshahi BangladeshDepartment of Biochemistry and Molecular Biology Faculty of Science University of Rajshahi Rajshahi BangladeshAbstract In the present study, Ag/AgCl‐NPs were biosynthesised using Hypnea musciformis seaweed extract; NPs synthesis was confirmed by a change of colour and observation of a razor‐sharp peak at 424 nm by UV–visible spectroscopy. Synthesised nanoparticles were characterised by transmission electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray powder diffraction and Fourier transform infrared spectroscopy. Bacterial cell growth inhibition proves that the Ag/AgCl‐NPs have strong antibacterial activity and cell morphological alteration was observed in treated bacterial cells using propidium iodide (PI). Ag/AgCl‐NPs inhibited Ehrlich ascites carcinoma (EAC) cells, colorectal cancer (HCT‐116) and breast cancer (MCF‐7) cell line in vitro with the IC50 values of 40.45, 24.08 and 36.95 μg/ml, respectively. Initiation of apoptosis in HCT‐116 and MCF‐7 cells was confirmed using PI, FITC‐annexin V and Hoechst 33342 dye. No reaction oxygen species generation was observed in both treated and untreated cell lines. A significant increase of ATG‐5 gene expression indicates the possibility of autophagy cell death besides apoptosis in MCF‐7 cells. The initiation of apoptosis in EAC cells was confirmed by observing caspase‐3 protein expression. Ag/AgCl‐NPs inhibited 22.83% and 51% of the EAC cell growth in vivo in mice when administered 1.5 and 3.0 mg/kg/day (i.p.), respectively, for 5 consequent days.https://doi.org/10.1049/nbt2.12075Ag/AgCl‐NPscell growth inhibitiongene expressionHypnea musciformismorphological study |
| spellingShingle | Rita Ghose A. K. M. Asaduzzaman Imtiaj Hasan Syed Rashel Kabir Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice IET Nanobiotechnology Ag/AgCl‐NPs cell growth inhibition gene expression Hypnea musciformis morphological study |
| title | Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice |
| title_full | Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice |
| title_fullStr | Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice |
| title_full_unstemmed | Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice |
| title_short | Hypnea musciformis‐mediated Ag/AgCl‐NPs inhibit pathogenic bacteria, HCT‐116 and MCF‐7 cells’ growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice |
| title_sort | hypnea musciformis mediated ag agcl nps inhibit pathogenic bacteria hct 116 and mcf 7 cells growth in vitro and ehrlich ascites carcinoma cells in vivo in mice |
| topic | Ag/AgCl‐NPs cell growth inhibition gene expression Hypnea musciformis morphological study |
| url | https://doi.org/10.1049/nbt2.12075 |
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