A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).
Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4222981?pdf=render |
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| author | Didi Zhang Yingpeng Hua Xiaohua Wang Hua Zhao Lei Shi Fangsen Xu |
| author_facet | Didi Zhang Yingpeng Hua Xiaohua Wang Hua Zhao Lei Shi Fangsen Xu |
| author_sort | Didi Zhang |
| collection | DOAJ |
| description | Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus. |
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| format | Article |
| id | doaj.art-fe17a7ccf22c4c51b6020623c3245ba3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T22:57:26Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe17a7ccf22c4c51b6020623c3245ba32022-12-22T03:13:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11208910.1371/journal.pone.0112089A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).Didi ZhangYingpeng HuaXiaohua WangHua ZhaoLei ShiFangsen XuLow boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.http://europepmc.org/articles/PMC4222981?pdf=render |
| spellingShingle | Didi Zhang Yingpeng Hua Xiaohua Wang Hua Zhao Lei Shi Fangsen Xu A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). PLoS ONE |
| title | A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). |
| title_full | A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). |
| title_fullStr | A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). |
| title_full_unstemmed | A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). |
| title_short | A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.). |
| title_sort | high density genetic map identifies a novel major qtl for boron efficiency in oilseed rape brassica napus l |
| url | http://europepmc.org/articles/PMC4222981?pdf=render |
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