Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers
A diabetic foot ulcer (DFUs) is a state of prolonged chronic inflammation, which can result in amputation. Different from normal skin wounds, various commercially available dressings have not sufficiently improved the healing of DFUs. In this study, a novel self-healing hydrogel was prepared by in s...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-07-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731420947242 |
| _version_ | 1817984390626541568 |
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| author | Haotian Bai Noh Kyu-Cheol Zhonghan Wang Yutao Cui He Liu Hou Liu Yubin Feng Yue Zhao Quan Lin Zuhao Li |
| author_facet | Haotian Bai Noh Kyu-Cheol Zhonghan Wang Yutao Cui He Liu Hou Liu Yubin Feng Yue Zhao Quan Lin Zuhao Li |
| author_sort | Haotian Bai |
| collection | DOAJ |
| description | A diabetic foot ulcer (DFUs) is a state of prolonged chronic inflammation, which can result in amputation. Different from normal skin wounds, various commercially available dressings have not sufficiently improved the healing of DFUs. In this study, a novel self-healing hydrogel was prepared by in situ crosslinking of N -carboxyethyl chitosan ( N -chitosan) and adipic acid dihydrazide (ADH) with hyaluronic acid-aldehyde (HA-ALD), to provide a moist and inflammatory relief environment to promote stem cell proliferation or secretion of growth factors, thus accelerating wound healing. The results demonstrated that this injectable and self-healing hydrogel has excellent swelling properties, stability, and mechanical properties. This biocompatible hydrogel stimulated secretion of growth factors from bone marrow mesenchymal stem cells (BM-MSCs) and regulated the inflammatory environment by inhibiting the expression of M1 macrophages and promoting the expression of M2 macrophages, resulting in granulation tissue formation, collagen deposition, nucleated cell proliferation, neovascularization, and enhanced diabetic wound healing. This study showed that N -chitosan/HA-ALD hydrogel could be used as a multifunctional injectable wound dressing to regulate chronic inflammation and provide an optimal environment for BM-MSCs to promote diabetic wound healing. |
| first_indexed | 2024-04-13T23:44:02Z |
| format | Article |
| id | doaj.art-fe1ac73613e44c45beaa958114935a48 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-13T23:44:02Z |
| publishDate | 2020-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-fe1ac73613e44c45beaa958114935a482022-12-22T02:24:24ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-07-011110.1177/2041731420947242Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcersHaotian Bai0Noh Kyu-Cheol1Zhonghan Wang2Yutao Cui3He Liu4Hou Liu5Yubin Feng6Yue Zhao7Quan Lin8Zuhao Li9Department of Orthopedics, Hallym University, Chuncheon, Gangwon-do, KoreaDepartment of Orthopedics, Hallym University, Chuncheon, Gangwon-do, KoreaDepartment of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin, P. R. ChinaState Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. ChinaState Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. ChinaState Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. ChinaState Key Lab of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, P. R. ChinaDepartment of Pain, Renji Hospital, South Campus, Shanghai Jiaotong University, Shanghai, P. R. ChinaA diabetic foot ulcer (DFUs) is a state of prolonged chronic inflammation, which can result in amputation. Different from normal skin wounds, various commercially available dressings have not sufficiently improved the healing of DFUs. In this study, a novel self-healing hydrogel was prepared by in situ crosslinking of N -carboxyethyl chitosan ( N -chitosan) and adipic acid dihydrazide (ADH) with hyaluronic acid-aldehyde (HA-ALD), to provide a moist and inflammatory relief environment to promote stem cell proliferation or secretion of growth factors, thus accelerating wound healing. The results demonstrated that this injectable and self-healing hydrogel has excellent swelling properties, stability, and mechanical properties. This biocompatible hydrogel stimulated secretion of growth factors from bone marrow mesenchymal stem cells (BM-MSCs) and regulated the inflammatory environment by inhibiting the expression of M1 macrophages and promoting the expression of M2 macrophages, resulting in granulation tissue formation, collagen deposition, nucleated cell proliferation, neovascularization, and enhanced diabetic wound healing. This study showed that N -chitosan/HA-ALD hydrogel could be used as a multifunctional injectable wound dressing to regulate chronic inflammation and provide an optimal environment for BM-MSCs to promote diabetic wound healing.https://doi.org/10.1177/2041731420947242 |
| spellingShingle | Haotian Bai Noh Kyu-Cheol Zhonghan Wang Yutao Cui He Liu Hou Liu Yubin Feng Yue Zhao Quan Lin Zuhao Li Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers Journal of Tissue Engineering |
| title | Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers |
| title_full | Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers |
| title_fullStr | Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers |
| title_full_unstemmed | Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers |
| title_short | Regulation of inflammatory microenvironment using a self-healing hydrogel loaded with BM-MSCs for advanced wound healing in rat diabetic foot ulcers |
| title_sort | regulation of inflammatory microenvironment using a self healing hydrogel loaded with bm mscs for advanced wound healing in rat diabetic foot ulcers |
| url | https://doi.org/10.1177/2041731420947242 |
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