Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking
Previous studies dealing with changes in microbial communities during vermicomposting were mostly performed at lab-scale conditions and by using low-throughput techniques. Therefore, we sought to characterize the bacterial succession during the vermicomposting of grape marc over a period of 91 days...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/10/473 |
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| author | María Gómez Brandón Manuel Aira Allison R. Kolbe Nariane de Andrade Marcos Pérez-Losada Jorge Domínguez |
| author_facet | María Gómez Brandón Manuel Aira Allison R. Kolbe Nariane de Andrade Marcos Pérez-Losada Jorge Domínguez |
| author_sort | María Gómez Brandón |
| collection | DOAJ |
| description | Previous studies dealing with changes in microbial communities during vermicomposting were mostly performed at lab-scale conditions and by using low-throughput techniques. Therefore, we sought to characterize the bacterial succession during the vermicomposting of grape marc over a period of 91 days in a pilot-scale vermireactor. Samples were taken at the initiation of vermicomposting, and days 14, 28, 42, and 91, representing both active and mature stages of vermicomposting. By using 16S rRNA high-throughput sequencing, significant changes in the bacterial community composition of grape marc were found after 14 days and throughout the process (<i>p</i> < 0.0001). There was also an increase in bacterial diversity, both taxonomic and phylogenetic, from day 14 until the end of the trial. We found the main core microbiome comprised of twelve bacterial taxa (~16.25% of the total sequences) known to be capable of nitrogen fixation and to confer plant-disease suppression. Accordingly, functional diversity included increases in specific genes related to nitrogen fixation and synthesis of plant hormones (salicylic acid) after 91 days. Together, the findings support the use of grape marc vermicompost for sustainable practices in the wine industry by disposing of this high-volume winery by-product and capturing its value to improve soil fertility. |
| first_indexed | 2024-12-22T10:23:10Z |
| format | Article |
| id | doaj.art-fe1ecc149f6b4811975916d44a09963e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-22T10:23:10Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fe1ecc149f6b4811975916d44a09963e2022-12-21T18:29:34ZengMDPI AGMicroorganisms2076-26072019-10-0171047310.3390/microorganisms7100473microorganisms7100473Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red WinemakingMaría Gómez Brandón0Manuel Aira1Allison R. Kolbe2Nariane de Andrade3Marcos Pérez-Losada4Jorge Domínguez5Grupo de Ecoloxía Animal (GEA), Universidade de Vigo, E-36310 Vigo, SpainGrupo de Ecoloxía Animal (GEA), Universidade de Vigo, E-36310 Vigo, SpainComputational Biology Institute, Milken Institute School of Public Health, George Washington University, Ashburn, VA 20147, USADepartamento de Solos, Universidade Federal de Santa María, CEP: 97105-900 Santa María, Río Grande do Sul, BrasilComputational Biology Institute, Milken Institute School of Public Health, George Washington University, Ashburn, VA 20147, USAGrupo de Ecoloxía Animal (GEA), Universidade de Vigo, E-36310 Vigo, SpainPrevious studies dealing with changes in microbial communities during vermicomposting were mostly performed at lab-scale conditions and by using low-throughput techniques. Therefore, we sought to characterize the bacterial succession during the vermicomposting of grape marc over a period of 91 days in a pilot-scale vermireactor. Samples were taken at the initiation of vermicomposting, and days 14, 28, 42, and 91, representing both active and mature stages of vermicomposting. By using 16S rRNA high-throughput sequencing, significant changes in the bacterial community composition of grape marc were found after 14 days and throughout the process (<i>p</i> < 0.0001). There was also an increase in bacterial diversity, both taxonomic and phylogenetic, from day 14 until the end of the trial. We found the main core microbiome comprised of twelve bacterial taxa (~16.25% of the total sequences) known to be capable of nitrogen fixation and to confer plant-disease suppression. Accordingly, functional diversity included increases in specific genes related to nitrogen fixation and synthesis of plant hormones (salicylic acid) after 91 days. Together, the findings support the use of grape marc vermicompost for sustainable practices in the wine industry by disposing of this high-volume winery by-product and capturing its value to improve soil fertility.https://www.mdpi.com/2076-2607/7/10/473winery wastesearthwormsmicrobial communitiesbacterial successioncore microbiomevermicompost |
| spellingShingle | María Gómez Brandón Manuel Aira Allison R. Kolbe Nariane de Andrade Marcos Pérez-Losada Jorge Domínguez Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking Microorganisms winery wastes earthworms microbial communities bacterial succession core microbiome vermicompost |
| title | Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking |
| title_full | Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking |
| title_fullStr | Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking |
| title_full_unstemmed | Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking |
| title_short | Rapid Bacterial Community Changes during Vermicomposting of Grape Marc Derived from Red Winemaking |
| title_sort | rapid bacterial community changes during vermicomposting of grape marc derived from red winemaking |
| topic | winery wastes earthworms microbial communities bacterial succession core microbiome vermicompost |
| url | https://www.mdpi.com/2076-2607/7/10/473 |
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