Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells
The effects of <i>Lycium barbarum</i> polysaccharides (LBP) and plasmon-activated water (PAW) against IFN-γ/TNF-α induced inflammation in human colon Caco-2 cells were investigated. Cells were divided into the control, induction, LBP treatment (100–500 μg/mL), and combination groups with...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/1424-8247/16/10/1455 |
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| author | Yu Zhi Lian Yu-Chuan Liu Chun-Chao Chang Tomonori Nochi Jane C.-J. Chao |
| author_facet | Yu Zhi Lian Yu-Chuan Liu Chun-Chao Chang Tomonori Nochi Jane C.-J. Chao |
| author_sort | Yu Zhi Lian |
| collection | DOAJ |
| description | The effects of <i>Lycium barbarum</i> polysaccharides (LBP) and plasmon-activated water (PAW) against IFN-γ/TNF-α induced inflammation in human colon Caco-2 cells were investigated. Cells were divided into the control, induction, LBP treatment (100–500 μg/mL), and combination groups with PAW. Inflammation was induced 24 h with 10 ng/mL IFN-γ when cell confluency reached >90%, and various doses of LBP with or without PAW were treated for 3 h, and subsequently 50 ng/mL TNF-α was added for another 24 h to provoke inflammation. Combination of LBP with PAW significantly decreased the secretion of IL-6 and IL-8. Cyclooxygenase-2 and inducible NO synthase expression was attenuated in all LBP-treated groups with or without PAW. NLRP3 inflammasome and related protein PYCARD expression were inhibited by LBP at the highest dose (500 μg/mL). All doses of LBP alone significantly decreased p-ERK expression, but combination with PAW increased p-ERK expression compared to those without PAW. Additionally, 250 and 500 μg/mL of LBP with or without PAW inhibited procaspase-3/caspase-3 expression. Therefore, LBP possesses anti-inflammation and anti-apoptosis by inhibiting the secretion of inflammatory cytokines and the expression of NLRP3 inflammasome-related protein. The combination with PAW exerts additive or synergistic effect on anti-inflammation. |
| first_indexed | 2024-03-10T20:59:14Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T20:59:14Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-fe234c036b804a668d0b3b498f47e4792023-11-19T17:42:45ZengMDPI AGPharmaceuticals1424-82472023-10-011610145510.3390/ph16101455Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 CellsYu Zhi Lian0Yu-Chuan Liu1Chun-Chao Chang2Tomonori Nochi3Jane C.-J. Chao4School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110301, TaiwanDepartment of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei 110301, TaiwanDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110301, TaiwanLaboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110301, TaiwanThe effects of <i>Lycium barbarum</i> polysaccharides (LBP) and plasmon-activated water (PAW) against IFN-γ/TNF-α induced inflammation in human colon Caco-2 cells were investigated. Cells were divided into the control, induction, LBP treatment (100–500 μg/mL), and combination groups with PAW. Inflammation was induced 24 h with 10 ng/mL IFN-γ when cell confluency reached >90%, and various doses of LBP with or without PAW were treated for 3 h, and subsequently 50 ng/mL TNF-α was added for another 24 h to provoke inflammation. Combination of LBP with PAW significantly decreased the secretion of IL-6 and IL-8. Cyclooxygenase-2 and inducible NO synthase expression was attenuated in all LBP-treated groups with or without PAW. NLRP3 inflammasome and related protein PYCARD expression were inhibited by LBP at the highest dose (500 μg/mL). All doses of LBP alone significantly decreased p-ERK expression, but combination with PAW increased p-ERK expression compared to those without PAW. Additionally, 250 and 500 μg/mL of LBP with or without PAW inhibited procaspase-3/caspase-3 expression. Therefore, LBP possesses anti-inflammation and anti-apoptosis by inhibiting the secretion of inflammatory cytokines and the expression of NLRP3 inflammasome-related protein. The combination with PAW exerts additive or synergistic effect on anti-inflammation.https://www.mdpi.com/1424-8247/16/10/1455ulcerative colitis<i>Lycium barbarum</i> polysaccharidesplasmon-activated waterinflammationapoptosis |
| spellingShingle | Yu Zhi Lian Yu-Chuan Liu Chun-Chao Chang Tomonori Nochi Jane C.-J. Chao Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells Pharmaceuticals ulcerative colitis <i>Lycium barbarum</i> polysaccharides plasmon-activated water inflammation apoptosis |
| title | Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells |
| title_full | Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells |
| title_fullStr | Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells |
| title_full_unstemmed | Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells |
| title_short | Combined <i>Lycium barbarum</i> Polysaccharides with Plasmon-Activated Water Affect IFN-γ/TNF-α Induced Inflammation in Caco-2 Cells |
| title_sort | combined i lycium barbarum i polysaccharides with plasmon activated water affect ifn γ tnf α induced inflammation in caco 2 cells |
| topic | ulcerative colitis <i>Lycium barbarum</i> polysaccharides plasmon-activated water inflammation apoptosis |
| url | https://www.mdpi.com/1424-8247/16/10/1455 |
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