Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, and cellular and humoral immunity is crucial to developing individual protection. Here, we aim to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/9/1916 |
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| author | Tamiris Azamor Ingrid Siciliano Horbach Danielle Brito e Cunha Juliana Gil Melgaço Andréa Marques Vieira da Silva Luciana Neves Tubarão Adriana de Souza Azevedo Renata Tourinho Santos Nathalia dos Santos Alves Thiago Lazari Machado Jane Silva Alessandro Fonseca de Souza Camilla Bayma Vanessa Pimenta Rocha Ana Beatriz Teixeira Frederico Brenda de Moura Dias Bruno Pimenta Setatino Caio Bidueira Denani Samir Pereira da Costa Campos Waleska Dias Schwarcz Michel Vergne Sucupira Edinea Pastro Mendes Edimilson Domingos da Silva Sheila Maria Barbosa de Lima Ana Paula Dinis Ano Bom Sotiris Missailidis |
| author_facet | Tamiris Azamor Ingrid Siciliano Horbach Danielle Brito e Cunha Juliana Gil Melgaço Andréa Marques Vieira da Silva Luciana Neves Tubarão Adriana de Souza Azevedo Renata Tourinho Santos Nathalia dos Santos Alves Thiago Lazari Machado Jane Silva Alessandro Fonseca de Souza Camilla Bayma Vanessa Pimenta Rocha Ana Beatriz Teixeira Frederico Brenda de Moura Dias Bruno Pimenta Setatino Caio Bidueira Denani Samir Pereira da Costa Campos Waleska Dias Schwarcz Michel Vergne Sucupira Edinea Pastro Mendes Edimilson Domingos da Silva Sheila Maria Barbosa de Lima Ana Paula Dinis Ano Bom Sotiris Missailidis |
| author_sort | Tamiris Azamor |
| collection | DOAJ |
| description | Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, and cellular and humoral immunity is crucial to developing individual protection. Here, we aim to investigate hybrid immunity against SARS-CoV-2 triggered by the ChAadOx1 nCoV-19 vaccine in a Brazilian cohort. We investigated the immune response from ChAadOx1 nCoV-19 vaccination in naïve (noCOVID-19) and previously infected individuals (COVID-19) by analyzing levels of D-dimers, total IgG, neutralizing antibodies (Nabs), IFN-γ (interferon-γ) secretion, and immunophenotyping of memory lymphocytes. No significant differences in D-dimer levels were observed 7 or 15 days after vaccination (DAV). All vaccinated individuals presented higher levels of total IgG or Nabs with a positive correlation (R = 0.88). Individuals in the COVID-19 group showed higher levels of antibody and memory B cells, with a faster antibody response starting at 7 DAV compared to noCOVID-19 at 15 DAV. Further, ChAadOx1 nCoV-19 vaccination led to enhanced IFN-γ production (15 DAV) and an increase in activated T CD4+ naïve cells in noCOVID-19 individuals in contrast with COVID-19 individuals. Hence, our data support that hybrid immunity triggered by ChAadOx1 nCoV-19 vaccination is associated with enhanced humoral response, together with a balanced cellular response. |
| first_indexed | 2024-03-09T22:14:51Z |
| format | Article |
| id | doaj.art-fe244ccc8f1747288177fdb243c76e94 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:14:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fe244ccc8f1747288177fdb243c76e942023-11-23T19:26:23ZengMDPI AGViruses1999-49152022-08-01149191610.3390/v14091916Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular InvestigationTamiris Azamor0Ingrid Siciliano Horbach1Danielle Brito e Cunha2Juliana Gil Melgaço3Andréa Marques Vieira da Silva4Luciana Neves Tubarão5Adriana de Souza Azevedo6Renata Tourinho Santos7Nathalia dos Santos Alves8Thiago Lazari Machado9Jane Silva10Alessandro Fonseca de Souza11Camilla Bayma12Vanessa Pimenta Rocha13Ana Beatriz Teixeira Frederico14Brenda de Moura Dias15Bruno Pimenta Setatino16Caio Bidueira Denani17Samir Pereira da Costa Campos18Waleska Dias Schwarcz19Michel Vergne Sucupira20Edinea Pastro Mendes21Edimilson Domingos da Silva22Sheila Maria Barbosa de Lima23Ana Paula Dinis Ano Bom24Sotiris Missailidis25Bio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilBio-Manguinhos/Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilInfections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, and cellular and humoral immunity is crucial to developing individual protection. Here, we aim to investigate hybrid immunity against SARS-CoV-2 triggered by the ChAadOx1 nCoV-19 vaccine in a Brazilian cohort. We investigated the immune response from ChAadOx1 nCoV-19 vaccination in naïve (noCOVID-19) and previously infected individuals (COVID-19) by analyzing levels of D-dimers, total IgG, neutralizing antibodies (Nabs), IFN-γ (interferon-γ) secretion, and immunophenotyping of memory lymphocytes. No significant differences in D-dimer levels were observed 7 or 15 days after vaccination (DAV). All vaccinated individuals presented higher levels of total IgG or Nabs with a positive correlation (R = 0.88). Individuals in the COVID-19 group showed higher levels of antibody and memory B cells, with a faster antibody response starting at 7 DAV compared to noCOVID-19 at 15 DAV. Further, ChAadOx1 nCoV-19 vaccination led to enhanced IFN-γ production (15 DAV) and an increase in activated T CD4+ naïve cells in noCOVID-19 individuals in contrast with COVID-19 individuals. Hence, our data support that hybrid immunity triggered by ChAadOx1 nCoV-19 vaccination is associated with enhanced humoral response, together with a balanced cellular response.https://www.mdpi.com/1999-4915/14/9/1916SARS-CoV-2 vaccineChAdOx1 nCoV-19hybrid immunitycellular and humoral immunity |
| spellingShingle | Tamiris Azamor Ingrid Siciliano Horbach Danielle Brito e Cunha Juliana Gil Melgaço Andréa Marques Vieira da Silva Luciana Neves Tubarão Adriana de Souza Azevedo Renata Tourinho Santos Nathalia dos Santos Alves Thiago Lazari Machado Jane Silva Alessandro Fonseca de Souza Camilla Bayma Vanessa Pimenta Rocha Ana Beatriz Teixeira Frederico Brenda de Moura Dias Bruno Pimenta Setatino Caio Bidueira Denani Samir Pereira da Costa Campos Waleska Dias Schwarcz Michel Vergne Sucupira Edinea Pastro Mendes Edimilson Domingos da Silva Sheila Maria Barbosa de Lima Ana Paula Dinis Ano Bom Sotiris Missailidis Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation Viruses SARS-CoV-2 vaccine ChAdOx1 nCoV-19 hybrid immunity cellular and humoral immunity |
| title | Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation |
| title_full | Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation |
| title_fullStr | Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation |
| title_full_unstemmed | Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation |
| title_short | Protective Immunity of COVID-19 Vaccination with ChAdOx1 nCoV-19 Following Previous SARS-CoV-2 Infection: A Humoral and Cellular Investigation |
| title_sort | protective immunity of covid 19 vaccination with chadox1 ncov 19 following previous sars cov 2 infection a humoral and cellular investigation |
| topic | SARS-CoV-2 vaccine ChAdOx1 nCoV-19 hybrid immunity cellular and humoral immunity |
| url | https://www.mdpi.com/1999-4915/14/9/1916 |
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