Signaling Modulations of miR-206-3p in Tooth Morphogenesis
MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell grow...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/15/5251 |
| Summary: | MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. The <i>miR-206-3p</i> has been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation of <i>miR-206-3p</i> during tooth morphogenesis using ex-vivo culture method. The expression pattern of <i>miR-206-3p</i> was examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression of <i>miR-206-3p</i> clearly altered expression patterns of dental-development–related signaling molecules, including <i>Axin2, Bmp2, Fgf4, Lef1</i> and <i>Shh</i>. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule–calcified teeth. Especially, mislocalization of β-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns of <i>Fgf4</i> and <i>Shh</i>. Overall, our data suggest that the <i>miR-206-3p</i> regulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown. |
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| ISSN: | 1661-6596 1422-0067 |