Signaling Modulations of miR-206-3p in Tooth Morphogenesis
MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell grow...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/15/5251 |
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| author | Sanjiv Neupane Yam Prasad Aryal Tae-Young Kim Chang-Yeol Yeon Chang-Hyeon An Ji-Youn Kim Hitoshi Yamamoto Youngkyun Lee Wern-Joo Sohn Jae-Young Kim |
| author_facet | Sanjiv Neupane Yam Prasad Aryal Tae-Young Kim Chang-Yeol Yeon Chang-Hyeon An Ji-Youn Kim Hitoshi Yamamoto Youngkyun Lee Wern-Joo Sohn Jae-Young Kim |
| author_sort | Sanjiv Neupane |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. The <i>miR-206-3p</i> has been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation of <i>miR-206-3p</i> during tooth morphogenesis using ex-vivo culture method. The expression pattern of <i>miR-206-3p</i> was examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression of <i>miR-206-3p</i> clearly altered expression patterns of dental-development–related signaling molecules, including <i>Axin2, Bmp2, Fgf4, Lef1</i> and <i>Shh</i>. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule–calcified teeth. Especially, mislocalization of β-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns of <i>Fgf4</i> and <i>Shh</i>. Overall, our data suggest that the <i>miR-206-3p</i> regulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown. |
| first_indexed | 2024-03-10T18:15:32Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:15:32Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fe27b8b9b56240b38d5184e81e6f5d392023-11-20T07:48:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115525110.3390/ijms21155251Signaling Modulations of miR-206-3p in Tooth MorphogenesisSanjiv Neupane0Yam Prasad Aryal1Tae-Young Kim2Chang-Yeol Yeon3Chang-Hyeon An4Ji-Youn Kim5Hitoshi Yamamoto6Youngkyun Lee7Wern-Joo Sohn8Jae-Young Kim9Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Oral and Maxillofacial Radiology, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaDepartment of Dental Hygiene, College of Health Science, Gachon University, Incheon 21936, KoreaDepartment of Histology and Developmental Biology, Tokyo Dental College, Tokyo 101-0061, JapanDepartment of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaPre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan 38610, KoreaDepartment of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaMicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. The <i>miR-206-3p</i> has been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation of <i>miR-206-3p</i> during tooth morphogenesis using ex-vivo culture method. The expression pattern of <i>miR-206-3p</i> was examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression of <i>miR-206-3p</i> clearly altered expression patterns of dental-development–related signaling molecules, including <i>Axin2, Bmp2, Fgf4, Lef1</i> and <i>Shh</i>. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule–calcified teeth. Especially, mislocalization of β-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns of <i>Fgf4</i> and <i>Shh</i>. Overall, our data suggest that the <i>miR-206-3p</i> regulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown.https://www.mdpi.com/1422-0067/21/15/5251epigenetic regulationtooth crown formationepithelial-mesenchymal interactionssignaling regulations |
| spellingShingle | Sanjiv Neupane Yam Prasad Aryal Tae-Young Kim Chang-Yeol Yeon Chang-Hyeon An Ji-Youn Kim Hitoshi Yamamoto Youngkyun Lee Wern-Joo Sohn Jae-Young Kim Signaling Modulations of miR-206-3p in Tooth Morphogenesis International Journal of Molecular Sciences epigenetic regulation tooth crown formation epithelial-mesenchymal interactions signaling regulations |
| title | Signaling Modulations of miR-206-3p in Tooth Morphogenesis |
| title_full | Signaling Modulations of miR-206-3p in Tooth Morphogenesis |
| title_fullStr | Signaling Modulations of miR-206-3p in Tooth Morphogenesis |
| title_full_unstemmed | Signaling Modulations of miR-206-3p in Tooth Morphogenesis |
| title_short | Signaling Modulations of miR-206-3p in Tooth Morphogenesis |
| title_sort | signaling modulations of mir 206 3p in tooth morphogenesis |
| topic | epigenetic regulation tooth crown formation epithelial-mesenchymal interactions signaling regulations |
| url | https://www.mdpi.com/1422-0067/21/15/5251 |
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