#43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions
Background and Aims: PCOS is an endocrine-related disease related to abnormal folliculogenesis and is a leading cause of infertility worldwide. Inhibition of GCs proliferation and increased GCs apoptosis have been identified as the major factors in aberrant follicle maturation. Recently, the roles o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2023-12-01
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| Series: | Fertility & Reproduction |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S2661318223743229 |
| _version_ | 1797237037451968512 |
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| author | Guoqing Zhang B. Hamdan Mukhri Lianwen Zheng |
| author_facet | Guoqing Zhang B. Hamdan Mukhri Lianwen Zheng |
| author_sort | Guoqing Zhang |
| collection | DOAJ |
| description | Background and Aims: PCOS is an endocrine-related disease related to abnormal folliculogenesis and is a leading cause of infertility worldwide. Inhibition of GCs proliferation and increased GCs apoptosis have been identified as the major factors in aberrant follicle maturation. Recently, the roles of miRNAs were identified in polycystic ovary syndrome (PCOS). For instance, miR-21-3p was identified as an important miRNA regulating autophagy of BGCs. Besides, studies showed that AMSC-EXOs can rescue the polycystic phenotype and metabolic dysfunction in PCOS ovaries by transferring miR-21-5p to the livers of rats with PCOS, finally improve reproduction. However, there is no research clarified the association between miRNA-21-3p and GCs function to date. Method: In the present study, letrozole was used to establish the PCOS rat model. Separate the ovary and then miRNA-21-3p expression will be analyzed by RT-PCR. Primary rat ovarian GCs were isolated and incubated and transfected with mimic or inhibitor of miRNA-21-3p, CCK8 kit was used to detect cell viability, PR-PCR was used to detect cell proliferation and apoptosis related gene expression. Finally, flowcytometry was used to test apoptosis rate of Gcs. Results: The expression of miR-21-3p in the ovaries of PCOS rats was significantly increased compared with that in control rats. Primary rat ovarian GCs were isolated and incubated successfully, and then CCK-8 experiment showed that GCs viability was significantly decreased when miR-21-3p was overexpressed. Additionally, the miR-21-3p mimic significantly decreased the expression of PCNA, Bcl-2, cell cycle-related genes (CDK2, CCNE1) and increased the expression of Bax and caspase3 in primary rat ovarian GCs. By contrast, transfected primary rat ovarian GCs using the miR-21-3p inhibitor significantly upregulated the expression of PCNA, Bcl-2, cell cycle-regulated genes (CDK2, CCNE1) and reduced the expression of Bax and caspase3. Finally, flow cytometry analysis demonstrated that overexpression of miR-21-3p significantly increased the apoptosis rate of GCs. By contrast, the suppression of miR-21-3p resulted in a significant decrease in the apoptosis rate of GCs. Conclusion: In PCOS rat model, miRNA-21-3p was proved significantly increased compared with that in control rats. Which is participant in the development of PCOS by regulating the function of granular cells. |
| first_indexed | 2024-04-24T17:13:23Z |
| format | Article |
| id | doaj.art-fe2b0707417644ae855ee87cd73b009c |
| institution | Directory Open Access Journal |
| issn | 2661-3182 2661-3174 |
| language | English |
| last_indexed | 2024-04-24T17:13:23Z |
| publishDate | 2023-12-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Fertility & Reproduction |
| spelling | doaj.art-fe2b0707417644ae855ee87cd73b009c2024-03-28T07:54:19ZengWorld Scientific PublishingFertility & Reproduction2661-31822661-31742023-12-01050458058010.1142/S2661318223743229#43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related FunctionsGuoqing Zhang0B. Hamdan Mukhri1Lianwen Zheng2Univeristi Malaya, Kuala Lumpur, MalaysiaUniveristi Malaya, Kuala Lumpur, MalaysiaThe Second Hospital of Jilin University, Changchun City, ChinaBackground and Aims: PCOS is an endocrine-related disease related to abnormal folliculogenesis and is a leading cause of infertility worldwide. Inhibition of GCs proliferation and increased GCs apoptosis have been identified as the major factors in aberrant follicle maturation. Recently, the roles of miRNAs were identified in polycystic ovary syndrome (PCOS). For instance, miR-21-3p was identified as an important miRNA regulating autophagy of BGCs. Besides, studies showed that AMSC-EXOs can rescue the polycystic phenotype and metabolic dysfunction in PCOS ovaries by transferring miR-21-5p to the livers of rats with PCOS, finally improve reproduction. However, there is no research clarified the association between miRNA-21-3p and GCs function to date. Method: In the present study, letrozole was used to establish the PCOS rat model. Separate the ovary and then miRNA-21-3p expression will be analyzed by RT-PCR. Primary rat ovarian GCs were isolated and incubated and transfected with mimic or inhibitor of miRNA-21-3p, CCK8 kit was used to detect cell viability, PR-PCR was used to detect cell proliferation and apoptosis related gene expression. Finally, flowcytometry was used to test apoptosis rate of Gcs. Results: The expression of miR-21-3p in the ovaries of PCOS rats was significantly increased compared with that in control rats. Primary rat ovarian GCs were isolated and incubated successfully, and then CCK-8 experiment showed that GCs viability was significantly decreased when miR-21-3p was overexpressed. Additionally, the miR-21-3p mimic significantly decreased the expression of PCNA, Bcl-2, cell cycle-related genes (CDK2, CCNE1) and increased the expression of Bax and caspase3 in primary rat ovarian GCs. By contrast, transfected primary rat ovarian GCs using the miR-21-3p inhibitor significantly upregulated the expression of PCNA, Bcl-2, cell cycle-regulated genes (CDK2, CCNE1) and reduced the expression of Bax and caspase3. Finally, flow cytometry analysis demonstrated that overexpression of miR-21-3p significantly increased the apoptosis rate of GCs. By contrast, the suppression of miR-21-3p resulted in a significant decrease in the apoptosis rate of GCs. Conclusion: In PCOS rat model, miRNA-21-3p was proved significantly increased compared with that in control rats. Which is participant in the development of PCOS by regulating the function of granular cells.https://www.worldscientific.com/doi/10.1142/S2661318223743229 |
| spellingShingle | Guoqing Zhang B. Hamdan Mukhri Lianwen Zheng #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions Fertility & Reproduction |
| title | #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions |
| title_full | #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions |
| title_fullStr | #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions |
| title_full_unstemmed | #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions |
| title_short | #43 : Expression of miRNA-21-3p in Polycystic Ovary Syndrome and its Related Functions |
| title_sort | 43 expression of mirna 21 3p in polycystic ovary syndrome and its related functions |
| url | https://www.worldscientific.com/doi/10.1142/S2661318223743229 |
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