Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis

Electrochemical microsensors are ideal to measure substances with low concentration in complex environments. The primary excitatory neurotransmitter l-glutamate is present in many foods as a distinctive flavour (enhancer) with a wide concentration range. In comparison to other methods, electrochemic...

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Main Authors: Andreas Weltin, Jochen Kieninger, Gerald A. Urban
Format: Article
Language:English
Published: MDPI AG 2017-08-01
Series:Proceedings
Subjects:
Online Access:https://www.mdpi.com/2504-3900/1/4/521
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author Andreas Weltin
Jochen Kieninger
Gerald A. Urban
author_facet Andreas Weltin
Jochen Kieninger
Gerald A. Urban
author_sort Andreas Weltin
collection DOAJ
description Electrochemical microsensors are ideal to measure substances with low concentration in complex environments. The primary excitatory neurotransmitter l-glutamate is present in many foods as a distinctive flavour (enhancer) with a wide concentration range. In comparison to other methods, electrochemical sensors allow the rapid, precise, cost-effective, online measurement without any sample treatment. We developed a disposable electrochemical microsensor platform with multiple integrated, highly sensitive (detection limit <150 nM) and selective enzyme-based glutamate biosensors. We showed both the precise determination of glutamate levels in processed foods with high glutamate content (15–40 mM), e.g., broth, and in foods with low natural concentrations such as different types of cow’s milk (~250 μM). Hereby, we successfully demonstrated the capabilities of electrochemical biosensors in food monitoring, analysis and quality control.
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spelling doaj.art-fe2b7a4635444fcf8de6164f8b3788742022-12-22T03:36:08ZengMDPI AGProceedings2504-39002017-08-011452110.3390/proceedings1040521proceedings1040521Highly Sensitive Electrochemical Glutamate Microsensors for Food AnalysisAndreas Weltin0Jochen Kieninger1Gerald A. Urban2Laboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Freiburg, GermanyElectrochemical microsensors are ideal to measure substances with low concentration in complex environments. The primary excitatory neurotransmitter l-glutamate is present in many foods as a distinctive flavour (enhancer) with a wide concentration range. In comparison to other methods, electrochemical sensors allow the rapid, precise, cost-effective, online measurement without any sample treatment. We developed a disposable electrochemical microsensor platform with multiple integrated, highly sensitive (detection limit <150 nM) and selective enzyme-based glutamate biosensors. We showed both the precise determination of glutamate levels in processed foods with high glutamate content (15–40 mM), e.g., broth, and in foods with low natural concentrations such as different types of cow’s milk (~250 μM). Hereby, we successfully demonstrated the capabilities of electrochemical biosensors in food monitoring, analysis and quality control.https://www.mdpi.com/2504-3900/1/4/521monosodium glutamateneurotransmitterbiosensorenzymefoodmilk
spellingShingle Andreas Weltin
Jochen Kieninger
Gerald A. Urban
Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
Proceedings
monosodium glutamate
neurotransmitter
biosensor
enzyme
food
milk
title Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
title_full Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
title_fullStr Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
title_full_unstemmed Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
title_short Highly Sensitive Electrochemical Glutamate Microsensors for Food Analysis
title_sort highly sensitive electrochemical glutamate microsensors for food analysis
topic monosodium glutamate
neurotransmitter
biosensor
enzyme
food
milk
url https://www.mdpi.com/2504-3900/1/4/521
work_keys_str_mv AT andreasweltin highlysensitiveelectrochemicalglutamatemicrosensorsforfoodanalysis
AT jochenkieninger highlysensitiveelectrochemicalglutamatemicrosensorsforfoodanalysis
AT geraldaurban highlysensitiveelectrochemicalglutamatemicrosensorsforfoodanalysis