Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles
This study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe<sub>2</sub>O<sub>4</sub>) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/23/17052 |
| _version_ | 1797400084269236224 |
|---|---|
| author | Karen L. Rincon-Granados América R. Vázquez-Olmos Adriana-Patricia Rodríguez-Hernández Gina Prado-Prone Margarita Rivera Vicente Garibay-Febles Yara C. Almanza-Arjona Roberto Y. Sato-Berrú Esther Mata-Zamora Phaedra S. Silva-Bermúdez Alejandro Vega-Jiménez |
| author_facet | Karen L. Rincon-Granados América R. Vázquez-Olmos Adriana-Patricia Rodríguez-Hernández Gina Prado-Prone Margarita Rivera Vicente Garibay-Febles Yara C. Almanza-Arjona Roberto Y. Sato-Berrú Esther Mata-Zamora Phaedra S. Silva-Bermúdez Alejandro Vega-Jiménez |
| author_sort | Karen L. Rincon-Granados |
| collection | DOAJ |
| description | This study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe<sub>2</sub>O<sub>4</sub>) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesize films of PP, PP@NiO, and PP@NiFe<sub>2</sub>O<sub>4</sub>, which were characterized by different spectroscopic and microscopic techniques. The X-ray diffraction (XRD) patterns confirmed that the small crystallite sizes of NiO and NiFe<sub>2</sub>O<sub>4</sub> NPs were maintained even after they were incorporated into the PP matrix. From the Raman scattering spectroscopy data, it was evident that there was a significant interaction between the NPs and the PP matrix. Additionally, the Scanning Electron Microscopy (SEM) analysis revealed a homogeneous dispersion of NiO and NiFe<sub>2</sub>O<sub>4</sub> NPs throughout the PP matrix. The incorporation of the NPs was observed to alter the surface roughness of the films; this behavior was studied by atomic force microscopy (AFM). The antibacterial properties of all films were evaluated against <i>Pseudomonas aeruginosa</i> (ATCC<sup>®</sup>: 43636™) and <i>Staphylococcus aureus</i> (ATCC<sup>®</sup>: 23235™), two opportunistic and nosocomial pathogens. The PP@NiO and PP@ NiFe<sub>2</sub>O<sub>4</sub> films showed over 90% bacterial growth inhibition for both strains. Additionally, the effects of the films on human skin cells, such as epidermal keratinocytes and dermal fibroblasts, were evaluated for cytotoxicity. The PP, PP@NiO, and PP@NiFe<sub>2</sub>O<sub>4</sub> films were nontoxic to human keratinocytes. Furthermore, compared to the PP film, improved biocompatibility of the PP@NiFe<sub>2</sub>O<sub>4</sub> film with human fibroblasts was observed. The methodology utilized in this study allows for the production of hybrid films that can inhibit the growth of Gram-positive bacteria, such as <i>S. aureus</i>, and Gram-negative bacteria, such as <i>P. aeruginosa</i>. These films have potential as coating materials to prevent bacterial proliferation on surfaces. |
| first_indexed | 2024-03-09T01:49:28Z |
| format | Article |
| id | doaj.art-fe2c8be6d731455a8ae742ea75251cf1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:49:28Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fe2c8be6d731455a8ae742ea75251cf12023-12-08T15:18:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124231705210.3390/ijms242317052Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> NanoparticlesKaren L. Rincon-Granados0América R. Vázquez-Olmos1Adriana-Patricia Rodríguez-Hernández2Gina Prado-Prone3Margarita Rivera4Vicente Garibay-Febles5Yara C. Almanza-Arjona6Roberto Y. Sato-Berrú7Esther Mata-Zamora8Phaedra S. Silva-Bermúdez9Alejandro Vega-Jiménez10Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México 04510, MexicoInstituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México 04510, MexicoLaboratorio de Genética Molecular, División de Estudios de Posgrado e Investigación de la Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoFacultad de Odontología, División de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México 04510, MexicoInstituto de Física, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoInstituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152 Col. San Bartolo Atepehuacan, Ciudad de México 07730, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey 64849, Nuevo León, MexicoInstituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México 04510, MexicoInstituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México 04510, MexicoUnidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México 14389, MexicoFacultad de Odontología, División de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México 04510, MexicoThis study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe<sub>2</sub>O<sub>4</sub>) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesize films of PP, PP@NiO, and PP@NiFe<sub>2</sub>O<sub>4</sub>, which were characterized by different spectroscopic and microscopic techniques. The X-ray diffraction (XRD) patterns confirmed that the small crystallite sizes of NiO and NiFe<sub>2</sub>O<sub>4</sub> NPs were maintained even after they were incorporated into the PP matrix. From the Raman scattering spectroscopy data, it was evident that there was a significant interaction between the NPs and the PP matrix. Additionally, the Scanning Electron Microscopy (SEM) analysis revealed a homogeneous dispersion of NiO and NiFe<sub>2</sub>O<sub>4</sub> NPs throughout the PP matrix. The incorporation of the NPs was observed to alter the surface roughness of the films; this behavior was studied by atomic force microscopy (AFM). The antibacterial properties of all films were evaluated against <i>Pseudomonas aeruginosa</i> (ATCC<sup>®</sup>: 43636™) and <i>Staphylococcus aureus</i> (ATCC<sup>®</sup>: 23235™), two opportunistic and nosocomial pathogens. The PP@NiO and PP@ NiFe<sub>2</sub>O<sub>4</sub> films showed over 90% bacterial growth inhibition for both strains. Additionally, the effects of the films on human skin cells, such as epidermal keratinocytes and dermal fibroblasts, were evaluated for cytotoxicity. The PP, PP@NiO, and PP@NiFe<sub>2</sub>O<sub>4</sub> films were nontoxic to human keratinocytes. Furthermore, compared to the PP film, improved biocompatibility of the PP@NiFe<sub>2</sub>O<sub>4</sub> film with human fibroblasts was observed. The methodology utilized in this study allows for the production of hybrid films that can inhibit the growth of Gram-positive bacteria, such as <i>S. aureus</i>, and Gram-negative bacteria, such as <i>P. aeruginosa</i>. These films have potential as coating materials to prevent bacterial proliferation on surfaces.https://www.mdpi.com/1422-0067/24/23/17052hybridfilmsnanoparticlespolypropyleneantibacterialcytotoxicity |
| spellingShingle | Karen L. Rincon-Granados América R. Vázquez-Olmos Adriana-Patricia Rodríguez-Hernández Gina Prado-Prone Margarita Rivera Vicente Garibay-Febles Yara C. Almanza-Arjona Roberto Y. Sato-Berrú Esther Mata-Zamora Phaedra S. Silva-Bermúdez Alejandro Vega-Jiménez Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles International Journal of Molecular Sciences hybrid films nanoparticles polypropylene antibacterial cytotoxicity |
| title | Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles |
| title_full | Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles |
| title_fullStr | Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles |
| title_full_unstemmed | Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles |
| title_short | Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe<sub>2</sub>O<sub>4</sub> Nanoparticles |
| title_sort | antibacterial and cytotoxic study of hybrid films based on polypropylene and nio or nife sub 2 sub o sub 4 sub nanoparticles |
| topic | hybrid films nanoparticles polypropylene antibacterial cytotoxicity |
| url | https://www.mdpi.com/1422-0067/24/23/17052 |
| work_keys_str_mv | AT karenlrincongranados antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT americarvazquezolmos antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT adrianapatriciarodriguezhernandez antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT ginapradoprone antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT margaritarivera antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT vicentegaribayfebles antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT yaracalmanzaarjona antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT robertoysatoberru antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT esthermatazamora antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT phaedrassilvabermudez antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles AT alejandrovegajimenez antibacterialandcytotoxicstudyofhybridfilmsbasedonpolypropyleneandnioornifesub2subosub4subnanoparticles |