Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11
The kinetoplastid membrane protein 11 (KMP-11) has been recently described in Leishmania (Leishmania) donovani as a major component of the promastigote membrane. Two oligonucleotide primers were synthesized to PCR-amplify the entire coding region of New World Leishmania species. The Leishmania (Vian...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1998-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200022&tlng=en |
| _version_ | 1797718192980754432 |
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| author | José R Ramírez Christof Berberich Andres Jaramillo Carlos Alonso Ivan D Vélez |
| author_facet | José R Ramírez Christof Berberich Andres Jaramillo Carlos Alonso Ivan D Vélez |
| author_sort | José R Ramírez |
| collection | DOAJ |
| description | The kinetoplastid membrane protein 11 (KMP-11) has been recently described in Leishmania (Leishmania) donovani as a major component of the promastigote membrane. Two oligonucleotide primers were synthesized to PCR-amplify the entire coding region of New World Leishmania species. The Leishmania (Viannia) panamensis amplification product was cloned, sequenced and the putative amino acid sequence determined. A remarkably high degree of sequence homology was observed with the corresponding molecule of L. (L) donovani and L. (L) infantum (97% and 96%, respectively). Southern blot analysis showed that the KMP-11 locus is conformed by three copies of the gene. The L. (V) panamensis ORF was subsequently cloned in a high expression vector and the recombinant protein was induced and purified from Escherichia coli cultures. Immunoblot analysis showed that 80%, 77% and 100% sera from cutaneous, mucocutaneous and visceral leishmaniasis patients, respectively, recognized the recombinant KMP-11 protein. In a similar assay, 86% of asymptomatic Leishmania-infected individuals showed IgG antibodies against the rKMP-11. We propose that KMP-11 could be used as a serologic marker for infection and disease caused by Leishmania in America. |
| first_indexed | 2024-03-12T08:47:21Z |
| format | Article |
| id | doaj.art-fe2d8768a7ce4751b833e37be14311ba |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:21Z |
| publishDate | 1998-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-fe2d8768a7ce4751b833e37be14311ba2023-09-02T16:25:28ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80601998-03-0193224725410.1590/S0074-02761998000200022Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11José R Ramírez0Christof Berberich1Andres Jaramillo2Carlos Alonso3Ivan D Vélez4Universidad de AntioquiaUniversidad de AntioquiaUniversidad de AntioquiaUniversidad Autónoma de MadridUniversidad de AntioquiaThe kinetoplastid membrane protein 11 (KMP-11) has been recently described in Leishmania (Leishmania) donovani as a major component of the promastigote membrane. Two oligonucleotide primers were synthesized to PCR-amplify the entire coding region of New World Leishmania species. The Leishmania (Viannia) panamensis amplification product was cloned, sequenced and the putative amino acid sequence determined. A remarkably high degree of sequence homology was observed with the corresponding molecule of L. (L) donovani and L. (L) infantum (97% and 96%, respectively). Southern blot analysis showed that the KMP-11 locus is conformed by three copies of the gene. The L. (V) panamensis ORF was subsequently cloned in a high expression vector and the recombinant protein was induced and purified from Escherichia coli cultures. Immunoblot analysis showed that 80%, 77% and 100% sera from cutaneous, mucocutaneous and visceral leishmaniasis patients, respectively, recognized the recombinant KMP-11 protein. In a similar assay, 86% of asymptomatic Leishmania-infected individuals showed IgG antibodies against the rKMP-11. We propose that KMP-11 could be used as a serologic marker for infection and disease caused by Leishmania in America.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200022&tlng=enNew World leishmaniasisLeishmania (Viannia) panamensiskinetoplastid membrane protein-11antigenicity |
| spellingShingle | José R Ramírez Christof Berberich Andres Jaramillo Carlos Alonso Ivan D Vélez Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 Memorias do Instituto Oswaldo Cruz New World leishmaniasis Leishmania (Viannia) panamensis kinetoplastid membrane protein-11 antigenicity |
| title | Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 |
| title_full | Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 |
| title_fullStr | Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 |
| title_full_unstemmed | Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 |
| title_short | Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11 |
| title_sort | molecular and antigenic characterization of the leishmania viannia panamensis kinetoplastid membrane protein 11 |
| topic | New World leishmaniasis Leishmania (Viannia) panamensis kinetoplastid membrane protein-11 antigenicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200022&tlng=en |
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