Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate.
Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in bas...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0285424 |
| _version_ | 1797802691330572288 |
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| author | Montana Renae Barrett Michael Scott Davis |
| author_facet | Montana Renae Barrett Michael Scott Davis |
| author_sort | Montana Renae Barrett |
| collection | DOAJ |
| description | Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals. |
| first_indexed | 2024-03-13T05:10:32Z |
| format | Article |
| id | doaj.art-fe2dc4b0e73e4c6e8b7566a718ba0cad |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T05:10:32Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe2dc4b0e73e4c6e8b7566a718ba0cad2023-06-16T05:31:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01185e028542410.1371/journal.pone.0285424Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate.Montana Renae BarrettMichael Scott DavisAthletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals.https://doi.org/10.1371/journal.pone.0285424 |
| spellingShingle | Montana Renae Barrett Michael Scott Davis Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. PLoS ONE |
| title | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. |
| title_full | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. |
| title_fullStr | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. |
| title_full_unstemmed | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. |
| title_short | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. |
| title_sort | conditioning induced expression of novel glucose transporters in canine skeletal muscle homogenate |
| url | https://doi.org/10.1371/journal.pone.0285424 |
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