Electrical detection of RNA cancer biomarkers at the single-molecule level
Abstract Cancer is a significant healthcare issue, and early screening methods based on biomarker analysis in liquid biopsies are promising avenues to reduce mortality rates. Electrical detection of nucleic acids at the single molecule level could enable these applications. We examine the electrical...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39450-6 |
| _version_ | 1797752809979904000 |
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| author | Keshani G. Gunasinghe Pattiya Arachchillage Subrata Chandra Ajoke Williams Patrick Piscitelli Jennifer Pham Aderlyn Castillo Lily Florence Srijith Rangan Juan M. Artes Vivancos |
| author_facet | Keshani G. Gunasinghe Pattiya Arachchillage Subrata Chandra Ajoke Williams Patrick Piscitelli Jennifer Pham Aderlyn Castillo Lily Florence Srijith Rangan Juan M. Artes Vivancos |
| author_sort | Keshani G. Gunasinghe Pattiya Arachchillage |
| collection | DOAJ |
| description | Abstract Cancer is a significant healthcare issue, and early screening methods based on biomarker analysis in liquid biopsies are promising avenues to reduce mortality rates. Electrical detection of nucleic acids at the single molecule level could enable these applications. We examine the electrical detection of RNA cancer biomarkers (KRAS mutants G12C and G12V) as a single-molecule proof-of-concept electrical biosensor for cancer screening applications. We show that the electrical conductance is highly sensitive to the sequence, allowing discrimination of the mutants from a wild-type KRAS sequence differing in just one base. In addition to this high specificity, our results also show that these biosensors are sensitive down to an individual molecule with a high signal-to-noise ratio. These results pave the way for future miniaturized single-molecule electrical biosensors that could be groundbreaking for cancer screening and other applications. |
| first_indexed | 2024-03-12T17:08:50Z |
| format | Article |
| id | doaj.art-fe32081483bd485ba3a75a85b528a811 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T17:08:50Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fe32081483bd485ba3a75a85b528a8112023-08-06T11:14:38ZengNature PortfolioScientific Reports2045-23222023-08-011311810.1038/s41598-023-39450-6Electrical detection of RNA cancer biomarkers at the single-molecule levelKeshani G. Gunasinghe Pattiya Arachchillage0Subrata Chandra1Ajoke Williams2Patrick Piscitelli3Jennifer Pham4Aderlyn Castillo5Lily Florence6Srijith Rangan7Juan M. Artes Vivancos8Department of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellDepartment of Chemistry, University of Massachusetts LowellAbstract Cancer is a significant healthcare issue, and early screening methods based on biomarker analysis in liquid biopsies are promising avenues to reduce mortality rates. Electrical detection of nucleic acids at the single molecule level could enable these applications. We examine the electrical detection of RNA cancer biomarkers (KRAS mutants G12C and G12V) as a single-molecule proof-of-concept electrical biosensor for cancer screening applications. We show that the electrical conductance is highly sensitive to the sequence, allowing discrimination of the mutants from a wild-type KRAS sequence differing in just one base. In addition to this high specificity, our results also show that these biosensors are sensitive down to an individual molecule with a high signal-to-noise ratio. These results pave the way for future miniaturized single-molecule electrical biosensors that could be groundbreaking for cancer screening and other applications.https://doi.org/10.1038/s41598-023-39450-6 |
| spellingShingle | Keshani G. Gunasinghe Pattiya Arachchillage Subrata Chandra Ajoke Williams Patrick Piscitelli Jennifer Pham Aderlyn Castillo Lily Florence Srijith Rangan Juan M. Artes Vivancos Electrical detection of RNA cancer biomarkers at the single-molecule level Scientific Reports |
| title | Electrical detection of RNA cancer biomarkers at the single-molecule level |
| title_full | Electrical detection of RNA cancer biomarkers at the single-molecule level |
| title_fullStr | Electrical detection of RNA cancer biomarkers at the single-molecule level |
| title_full_unstemmed | Electrical detection of RNA cancer biomarkers at the single-molecule level |
| title_short | Electrical detection of RNA cancer biomarkers at the single-molecule level |
| title_sort | electrical detection of rna cancer biomarkers at the single molecule level |
| url | https://doi.org/10.1038/s41598-023-39450-6 |
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