CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS
Introduction. According to modern perceptions, tumor growth, along with oxidative stress formation, is accompanied by hypoxia. Nowadays studying the regulation of cellular molecular system functioning by conformational changes in proteins appears to be a topical issue. Research goal was to evaluate...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2015-08-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/206 |
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| author | D. S. Orlov N. V. Ryazantseva Ye. A. Stepovaya O. L. Nosareva Ye. V. Shakhristova V. V. Ivanov |
| author_facet | D. S. Orlov N. V. Ryazantseva Ye. A. Stepovaya O. L. Nosareva Ye. V. Shakhristova V. V. Ivanov |
| author_sort | D. S. Orlov |
| collection | DOAJ |
| description | Introduction. According to modern perceptions, tumor growth, along with oxidative stress formation, is accompanied by hypoxia. Nowadays studying the regulation of cellular molecular system functioning by conformational changes in proteins appears to be a topical issue. Research goal was to evaluate the state of the glutathione system and the level of protein glutathionylation in P19 embryonal carcinoma (EC) cells under hypoxic conditions.Material and methods. P19 EC cells (mouse embryonal carcinoma) cultured under normoxic and hypox-ic conditions served the research material.The concentration of total, oxidized, reduced and protein-bound glutathione, the reduced to oxidized thiol ratio as well as glutathione peroxidase and glutathione reductase activity were determined by spectropho-tometry.Results. Glutathione imbalance was accompanied by a decrease in P19 EC cell redox status under hypox-ic conditions against the backdrop of a rise in protein-bound glutathione.Conclusions. As a result of the conducted study oxidative stress formation was identified when modeling hypoxia in P19 embryonal carcinoma cells. The rise in the concentration of protein-bound glutathione may indicate the role of protein glutathionylation in regulation of P19 cell metabolism and functions un-der hypoxia. |
| first_indexed | 2024-04-10T01:08:13Z |
| format | Article |
| id | doaj.art-fe375a401d6a449790343e91d82e7b8d |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:08:13Z |
| publishDate | 2015-08-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-fe375a401d6a449790343e91d82e7b8d2023-03-13T09:58:21ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842015-08-01144414510.20538/1682-0363-2015-4-41-45204CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONSD. S. Orlov0N. V. Ryazantseva1Ye. A. Stepovaya2O. L. Nosareva3Ye. V. Shakhristova4V. V. Ivanov5Сибирский государственный медицинский университет, ТомскСибирский федеральный университет, Красноярск; Красноярский государственный медицинский университет им. проф. В.Ф. Войно-ЯсенецкогоСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскIntroduction. According to modern perceptions, tumor growth, along with oxidative stress formation, is accompanied by hypoxia. Nowadays studying the regulation of cellular molecular system functioning by conformational changes in proteins appears to be a topical issue. Research goal was to evaluate the state of the glutathione system and the level of protein glutathionylation in P19 embryonal carcinoma (EC) cells under hypoxic conditions.Material and methods. P19 EC cells (mouse embryonal carcinoma) cultured under normoxic and hypox-ic conditions served the research material.The concentration of total, oxidized, reduced and protein-bound glutathione, the reduced to oxidized thiol ratio as well as glutathione peroxidase and glutathione reductase activity were determined by spectropho-tometry.Results. Glutathione imbalance was accompanied by a decrease in P19 EC cell redox status under hypox-ic conditions against the backdrop of a rise in protein-bound glutathione.Conclusions. As a result of the conducted study oxidative stress formation was identified when modeling hypoxia in P19 embryonal carcinoma cells. The rise in the concentration of protein-bound glutathione may indicate the role of protein glutathionylation in regulation of P19 cell metabolism and functions un-der hypoxia.https://bulletin.ssmu.ru/jour/article/view/206глутатионгипоксияокислительный стрессопухолевый рост |
| spellingShingle | D. S. Orlov N. V. Ryazantseva Ye. A. Stepovaya O. L. Nosareva Ye. V. Shakhristova V. V. Ivanov CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS Бюллетень сибирской медицины глутатион гипоксия окислительный стресс опухолевый рост |
| title | CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS |
| title_full | CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS |
| title_fullStr | CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS |
| title_full_unstemmed | CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS |
| title_short | CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS |
| title_sort | changes in the glutathione system in p19 embryonal carcinoma cells under hypoxic conditions |
| topic | глутатион гипоксия окислительный стресс опухолевый рост |
| url | https://bulletin.ssmu.ru/jour/article/view/206 |
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