Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage
Phytoplasmas are obligate, phloem-restricted phytopathogens that are disseminated by phloem-sap-sucking insects. Phytoplasma infection severely impairs assimilate translocation in host plants and might be responsible for massive changes in phloem physiology. Methods to study phytoplasma- induced cha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2013-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-12-0207-R |
| _version_ | 1818915254762995712 |
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| author | Rita Musetti Stefanie V. Buxa Federica De Marco Alberto Loschi Rachele Polizzotto Karl-Heinz Kogel Aart J. E. van Bel |
| author_facet | Rita Musetti Stefanie V. Buxa Federica De Marco Alberto Loschi Rachele Polizzotto Karl-Heinz Kogel Aart J. E. van Bel |
| author_sort | Rita Musetti |
| collection | DOAJ |
| description | Phytoplasmas are obligate, phloem-restricted phytopathogens that are disseminated by phloem-sap-sucking insects. Phytoplasma infection severely impairs assimilate translocation in host plants and might be responsible for massive changes in phloem physiology. Methods to study phytoplasma- induced changes thus far provoked massive, native occlusion artifacts in sieve tubes. Hence, phytoplasma-phloem relationships were investigated here in intact Vicia faba host plants using a set of vital fluorescent probes and confocal laser-scanning microscopy. We focused on the effects of phytoplasma infection on phloem mass-flow performance and evaluated whether phytoplasmas induce sieve-plate occlusion. Apparently, phytoplasma infection brings about Ca2+ influx into sieve tubes, leading to sieve-plate occlusion by callose deposition or protein plugging. In addition, Ca2+ influx may confer cell wall thickening of conducting elements. In conclusion, phytoplasma effectors may cause gating of sieve-element Ca2+ channels leading to sieve-tube occlusion with presumptive dramatic effects on phytoplasma spread and photoassimilate distribution. |
| first_indexed | 2024-12-19T23:59:22Z |
| format | Article |
| id | doaj.art-fe39ded8aad94288bbac821def781ccd |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:59:22Z |
| publishDate | 2013-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-fe39ded8aad94288bbac821def781ccd2022-12-21T20:00:54ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062013-04-0126437938610.1094/MPMI-08-12-0207-RPhytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube BlockageRita MusettiStefanie V. BuxaFederica De MarcoAlberto LoschiRachele PolizzottoKarl-Heinz KogelAart J. E. van BelPhytoplasmas are obligate, phloem-restricted phytopathogens that are disseminated by phloem-sap-sucking insects. Phytoplasma infection severely impairs assimilate translocation in host plants and might be responsible for massive changes in phloem physiology. Methods to study phytoplasma- induced changes thus far provoked massive, native occlusion artifacts in sieve tubes. Hence, phytoplasma-phloem relationships were investigated here in intact Vicia faba host plants using a set of vital fluorescent probes and confocal laser-scanning microscopy. We focused on the effects of phytoplasma infection on phloem mass-flow performance and evaluated whether phytoplasmas induce sieve-plate occlusion. Apparently, phytoplasma infection brings about Ca2+ influx into sieve tubes, leading to sieve-plate occlusion by callose deposition or protein plugging. In addition, Ca2+ influx may confer cell wall thickening of conducting elements. In conclusion, phytoplasma effectors may cause gating of sieve-element Ca2+ channels leading to sieve-tube occlusion with presumptive dramatic effects on phytoplasma spread and photoassimilate distribution.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-12-0207-R |
| spellingShingle | Rita Musetti Stefanie V. Buxa Federica De Marco Alberto Loschi Rachele Polizzotto Karl-Heinz Kogel Aart J. E. van Bel Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage Molecular Plant-Microbe Interactions |
| title | Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage |
| title_full | Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage |
| title_fullStr | Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage |
| title_full_unstemmed | Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage |
| title_short | Phytoplasma-Triggered Ca2+ Influx Is Involved in Sieve-Tube Blockage |
| title_sort | phytoplasma triggered ca2 influx is involved in sieve tube blockage |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-12-0207-R |
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