Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis
Abstract Legume plants establish a nitrogen-fixing symbiosis with soil bacteria known as rhizobia. Compatibility between legumes and rhizobia is determined at species-specific level, but variations in the outcome of the symbiotic process are also influenced by the capacity of the plant to discrimina...
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| Format: | Article |
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Nature Portfolio
2022-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-06566-0 |
| _version_ | 1819277948171059200 |
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| author | Joaquín Clúa Claudio Hernán Rivero Carla Roda Carolina Giorgis Soledad Donna María Eugenia Zanetti Flavio Antonio Blanco |
| author_facet | Joaquín Clúa Claudio Hernán Rivero Carla Roda Carolina Giorgis Soledad Donna María Eugenia Zanetti Flavio Antonio Blanco |
| author_sort | Joaquín Clúa |
| collection | DOAJ |
| description | Abstract Legume plants establish a nitrogen-fixing symbiosis with soil bacteria known as rhizobia. Compatibility between legumes and rhizobia is determined at species-specific level, but variations in the outcome of the symbiotic process are also influenced by the capacity of the plant to discriminate and select specific strains that are better partners. We compared the transcriptional response of two genetically diverse accessions of Phaseolus vulgaris from Mesoamerica and South Andes to Rhizobium etli strains that exhibit variable degrees of symbiotic affinities. Our results indicate that the plant genotype is the major determinant of the transcriptional reprogramming occurring in roots at early stages of the symbiotic interaction. Differentially expressed genes (DEGs) regulated in the Mesoamerican and the Andean accessions in response to specific strains are different, but they belong to the same functional categories. The common and strain-specific transcriptional responses to rhizobia involve distinct transcription factors and cis-elements present in the promoters of DEGs in each accession, showing that diversification and domestication of common bean at different geographic regions influenced the evolution of symbiosis differently in each genetic pool. Quantitative PCR analysis validated our transcriptional datasets, which constitute a valuable source of coding and non-coding candidate genes to further unravel the molecular determinants governing the mechanisms by which plants select bacterial strains that produce a better symbiotic outcome. |
| first_indexed | 2024-12-24T00:04:13Z |
| format | Article |
| id | doaj.art-fe3c3ce7d8b8448383cd67f3a102135b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-24T00:04:13Z |
| publishDate | 2022-02-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-fe3c3ce7d8b8448383cd67f3a102135b2022-12-21T17:25:04ZengNature PortfolioScientific Reports2045-23222022-02-0112111510.1038/s41598-022-06566-0Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosisJoaquín Clúa0Claudio Hernán Rivero1Carla Roda2Carolina Giorgis3Soledad Donna4María Eugenia Zanetti5Flavio Antonio Blanco6Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataInstituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La PlataAbstract Legume plants establish a nitrogen-fixing symbiosis with soil bacteria known as rhizobia. Compatibility between legumes and rhizobia is determined at species-specific level, but variations in the outcome of the symbiotic process are also influenced by the capacity of the plant to discriminate and select specific strains that are better partners. We compared the transcriptional response of two genetically diverse accessions of Phaseolus vulgaris from Mesoamerica and South Andes to Rhizobium etli strains that exhibit variable degrees of symbiotic affinities. Our results indicate that the plant genotype is the major determinant of the transcriptional reprogramming occurring in roots at early stages of the symbiotic interaction. Differentially expressed genes (DEGs) regulated in the Mesoamerican and the Andean accessions in response to specific strains are different, but they belong to the same functional categories. The common and strain-specific transcriptional responses to rhizobia involve distinct transcription factors and cis-elements present in the promoters of DEGs in each accession, showing that diversification and domestication of common bean at different geographic regions influenced the evolution of symbiosis differently in each genetic pool. Quantitative PCR analysis validated our transcriptional datasets, which constitute a valuable source of coding and non-coding candidate genes to further unravel the molecular determinants governing the mechanisms by which plants select bacterial strains that produce a better symbiotic outcome.https://doi.org/10.1038/s41598-022-06566-0 |
| spellingShingle | Joaquín Clúa Claudio Hernán Rivero Carla Roda Carolina Giorgis Soledad Donna María Eugenia Zanetti Flavio Antonio Blanco Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis Scientific Reports |
| title | Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis |
| title_full | Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis |
| title_fullStr | Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis |
| title_full_unstemmed | Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis |
| title_short | Transcriptomic analysis of Mesoamerican and Andean Phaseolus vulgaris accessions revealed mRNAs and lncRNAs associated with strain selectivity during symbiosis |
| title_sort | transcriptomic analysis of mesoamerican and andean phaseolus vulgaris accessions revealed mrnas and lncrnas associated with strain selectivity during symbiosis |
| url | https://doi.org/10.1038/s41598-022-06566-0 |
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