New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts
Evidence showed that N6-methyladenosine (m6A) modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species. However, no information regarding m6A modification in Eimeria tenella is currently available. In the presen...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923002265 |
| _version_ | 1797356375817322496 |
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| author | Qing Liu Bingjin Mu Yijing Meng Linmei Yu Zirui Wang Tao Jia Wenbin Zheng Wenwei Gao Shichen Xie Xingquan Zhu |
| author_facet | Qing Liu Bingjin Mu Yijing Meng Linmei Yu Zirui Wang Tao Jia Wenbin Zheng Wenwei Gao Shichen Xie Xingquan Zhu |
| author_sort | Qing Liu |
| collection | DOAJ |
| description | Evidence showed that N6-methyladenosine (m6A) modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species. However, no information regarding m6A modification in Eimeria tenella is currently available. In the present study, we surveyed the transcriptome-wide prevalence of m6A in sporulated oocysts and unsporulated oocysts of E. tenella. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) analysis showed that m6A modification was most abundant in the coding sequences, followed by stop codon. There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts. Further joint analysis suggested that m6A modification of the majority of genes was positively correlated with mRNA expression. The mRNA relative expression and m6A level of the selected genes were confirmed by quantitative reverse transcription PCR (RT-qPCR) and MeRIP-qPCR. GO and KEGG analysis indicated that differentially m6A methylated genes (DMMGs) with significant differences in mRNA expression were closely related to processes such as regulation of gene expression, epigenetic, microtubule, autophagy-other and TOR signaling. Moreover, a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level. GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E. tenella. We firstly present a map of RNA m6A modification in E. tenella, which provides significant insights into developmental biology of E. tenella. |
| first_indexed | 2024-03-08T14:26:43Z |
| format | Article |
| id | doaj.art-fe429781842e47cfbd07bc3f45545025 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-08T14:26:43Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-fe429781842e47cfbd07bc3f455450252024-01-13T04:44:04ZengElsevierJournal of Integrative Agriculture2095-31192024-01-01231239250New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocystsQing Liu0Bingjin Mu1Yijing Meng2Linmei Yu3Zirui Wang4Tao Jia5Wenbin Zheng6Wenwei Gao7Shichen Xie8Xingquan Zhu9Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China; Correspondence Shichen Xie, Tel: +86-354-6286886Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; Correspondence Xingquan Zhu, Tel: +86-354-6286886Evidence showed that N6-methyladenosine (m6A) modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species. However, no information regarding m6A modification in Eimeria tenella is currently available. In the present study, we surveyed the transcriptome-wide prevalence of m6A in sporulated oocysts and unsporulated oocysts of E. tenella. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) analysis showed that m6A modification was most abundant in the coding sequences, followed by stop codon. There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts. Further joint analysis suggested that m6A modification of the majority of genes was positively correlated with mRNA expression. The mRNA relative expression and m6A level of the selected genes were confirmed by quantitative reverse transcription PCR (RT-qPCR) and MeRIP-qPCR. GO and KEGG analysis indicated that differentially m6A methylated genes (DMMGs) with significant differences in mRNA expression were closely related to processes such as regulation of gene expression, epigenetic, microtubule, autophagy-other and TOR signaling. Moreover, a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level. GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E. tenella. We firstly present a map of RNA m6A modification in E. tenella, which provides significant insights into developmental biology of E. tenella.http://www.sciencedirect.com/science/article/pii/S2095311923002265Eimeria tenellam6ARNA methylationMeRIP-seqRNA-seqproteomic analysis |
| spellingShingle | Qing Liu Bingjin Mu Yijing Meng Linmei Yu Zirui Wang Tao Jia Wenbin Zheng Wenwei Gao Shichen Xie Xingquan Zhu New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts Journal of Integrative Agriculture Eimeria tenella m6A RNA methylation MeRIP-seq RNA-seq proteomic analysis |
| title | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| title_full | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| title_fullStr | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| title_full_unstemmed | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| title_short | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| title_sort | new insights into developmental biology of eimeria tenella revealed by comparative analysis of mrna n6 methyladenosine modification between unsporulated oocysts and sporulated oocysts |
| topic | Eimeria tenella m6A RNA methylation MeRIP-seq RNA-seq proteomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923002265 |
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