T4 phage and its head surface proteins do not stimulate inflammatory mediator production.
Viruses are potent activators of the signal pathways leading to increased cytokine or ROS production. The effects exerted on the immune system are usually mediated by viral proteins. Complementary to the progress in phage therapy practice, advancement of knowledge about the influence of bacteriophag...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3745418?pdf=render |
| _version_ | 1818529583590277120 |
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| author | Paulina Miernikiewicz Krystyna Dąbrowska Agnieszka Piotrowicz Barbara Owczarek Justyna Wojas-Turek Jagoda Kicielińska Joanna Rossowska Elżbieta Pajtasz-Piasecka Katarzyna Hodyra Katarzyna Macegoniuk Kamila Rzewucka Agnieszka Kopciuch Tomasz Majka Andrey Letarov Eugene Kulikov Henryk Maciejewski Andrzej Górski |
| author_facet | Paulina Miernikiewicz Krystyna Dąbrowska Agnieszka Piotrowicz Barbara Owczarek Justyna Wojas-Turek Jagoda Kicielińska Joanna Rossowska Elżbieta Pajtasz-Piasecka Katarzyna Hodyra Katarzyna Macegoniuk Kamila Rzewucka Agnieszka Kopciuch Tomasz Majka Andrey Letarov Eugene Kulikov Henryk Maciejewski Andrzej Górski |
| author_sort | Paulina Miernikiewicz |
| collection | DOAJ |
| description | Viruses are potent activators of the signal pathways leading to increased cytokine or ROS production. The effects exerted on the immune system are usually mediated by viral proteins. Complementary to the progress in phage therapy practice, advancement of knowledge about the influence of bacteriophages on mammalian immunity is necessary. Particularly, the potential ability of phage proteins to act like other viral stimulators of the immune system may have strong practical implications for the safety and efficacy of bacteriophage therapy. Here we present studies on the effect of T4 phage and its head proteins on production of inflammatory mediators and inflammation-related factors: IL-1α, IL-1β, IL-2, IL-6, IL-10, IL-12 p40/p70, IFN-γ, TNF-α, MCP-1, MIG, RANTES, GCSF, GM-CSF and reactive oxygen species (ROS). Plasma cytokine profiles in an in vivo mouse model and in human blood cells treated with gp23*, gp24*, Hoc and Soc were evaluated by cytokine antibody arrays. Cytokine production and expression of CD40, CD80, CD86 and MHC class II molecules were also investigated in mouse bone marrow-derived dendritic cells treated with whole T4 phage particle or the same capsid proteins. The influence of T4 and gp23*, gp24*, Hoc and Soc on reactive oxygen species generation was examined in blood cells using luminol-dependent chemiluminescence assay. In all performed assays, the T4 bacteriophage and its capsid proteins gp23*, gp24*, Hoc and Soc did not affect production of inflammatory-related cytokines or ROS. These observations are of importance for any medical or veterinary application of bacteriophages. |
| first_indexed | 2024-12-11T17:08:43Z |
| format | Article |
| id | doaj.art-fe447750e2e54e858c8c491766678661 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T17:08:43Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe447750e2e54e858c8c4917666786612022-12-22T00:57:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7103610.1371/journal.pone.0071036T4 phage and its head surface proteins do not stimulate inflammatory mediator production.Paulina MiernikiewiczKrystyna DąbrowskaAgnieszka PiotrowiczBarbara OwczarekJustyna Wojas-TurekJagoda KicielińskaJoanna RossowskaElżbieta Pajtasz-PiaseckaKatarzyna HodyraKatarzyna MacegoniukKamila RzewuckaAgnieszka KopciuchTomasz MajkaAndrey LetarovEugene KulikovHenryk MaciejewskiAndrzej GórskiViruses are potent activators of the signal pathways leading to increased cytokine or ROS production. The effects exerted on the immune system are usually mediated by viral proteins. Complementary to the progress in phage therapy practice, advancement of knowledge about the influence of bacteriophages on mammalian immunity is necessary. Particularly, the potential ability of phage proteins to act like other viral stimulators of the immune system may have strong practical implications for the safety and efficacy of bacteriophage therapy. Here we present studies on the effect of T4 phage and its head proteins on production of inflammatory mediators and inflammation-related factors: IL-1α, IL-1β, IL-2, IL-6, IL-10, IL-12 p40/p70, IFN-γ, TNF-α, MCP-1, MIG, RANTES, GCSF, GM-CSF and reactive oxygen species (ROS). Plasma cytokine profiles in an in vivo mouse model and in human blood cells treated with gp23*, gp24*, Hoc and Soc were evaluated by cytokine antibody arrays. Cytokine production and expression of CD40, CD80, CD86 and MHC class II molecules were also investigated in mouse bone marrow-derived dendritic cells treated with whole T4 phage particle or the same capsid proteins. The influence of T4 and gp23*, gp24*, Hoc and Soc on reactive oxygen species generation was examined in blood cells using luminol-dependent chemiluminescence assay. In all performed assays, the T4 bacteriophage and its capsid proteins gp23*, gp24*, Hoc and Soc did not affect production of inflammatory-related cytokines or ROS. These observations are of importance for any medical or veterinary application of bacteriophages.http://europepmc.org/articles/PMC3745418?pdf=render |
| spellingShingle | Paulina Miernikiewicz Krystyna Dąbrowska Agnieszka Piotrowicz Barbara Owczarek Justyna Wojas-Turek Jagoda Kicielińska Joanna Rossowska Elżbieta Pajtasz-Piasecka Katarzyna Hodyra Katarzyna Macegoniuk Kamila Rzewucka Agnieszka Kopciuch Tomasz Majka Andrey Letarov Eugene Kulikov Henryk Maciejewski Andrzej Górski T4 phage and its head surface proteins do not stimulate inflammatory mediator production. PLoS ONE |
| title | T4 phage and its head surface proteins do not stimulate inflammatory mediator production. |
| title_full | T4 phage and its head surface proteins do not stimulate inflammatory mediator production. |
| title_fullStr | T4 phage and its head surface proteins do not stimulate inflammatory mediator production. |
| title_full_unstemmed | T4 phage and its head surface proteins do not stimulate inflammatory mediator production. |
| title_short | T4 phage and its head surface proteins do not stimulate inflammatory mediator production. |
| title_sort | t4 phage and its head surface proteins do not stimulate inflammatory mediator production |
| url | http://europepmc.org/articles/PMC3745418?pdf=render |
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