Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010
<p>Abstract</p> <p>Background</p> <p>Certain adenovirus serotypes cause severe infections, especially in children. It is important to monitor temporal changes in serotypes causing clinical disease. The objective of this study was to document circulating respiratory aden...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2013-01-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://www.virologyj.com/content/10/1/15 |
| _version_ | 1818787046340165632 |
|---|---|
| author | Abbas Kaniza Zahra Lombos Ernesto Duvvuri Venkata R Olsha Romy Higgins Rachel R Gubbay Jonathan B |
| author_facet | Abbas Kaniza Zahra Lombos Ernesto Duvvuri Venkata R Olsha Romy Higgins Rachel R Gubbay Jonathan B |
| author_sort | Abbas Kaniza Zahra |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Certain adenovirus serotypes cause severe infections, especially in children. It is important to monitor temporal changes in serotypes causing clinical disease. The objective of this study was to document circulating respiratory adenovirus serotypes by sequencing adenovirus culture isolates from the Greater Toronto Area, Ontario, during December 2008 to April 2010.</p> <p>Methods</p> <p>Nucleic acid extraction was performed on 90 respiratory tract adenovirus culture isolates. PCR amplification was conducted with primers targeting the adenovirus hexon gene hypervariable region 7. Sanger sequencing and phylogenetic analyses were performed to determine serotype identities.</p> <p>Results</p> <p>Among 90 clinical respiratory isolates sequenced, eight different serotypes were identified. Serotype 3 (34, 38%), serotype 2 (30, 30%), and serotype 1 (14, 16%) isolates were most common; serotypes 5, 6, 11, 17 and 21 were also observed. Seventeen (50%) of the 34 HAdV-3 isolates were identified between December 2008 and February 2009, while none were identified from December 2009 to February 2010. Between December 2008 and April 2009, the two most common serotypes were HAdV-3 and HAdV-2, detected in 18 (53%) and 8 (24%) of the 34 cultures isolates, respectively. However, from December 2009 to April 2010, there was an increase in HAdV-2, which became the most prevalent serotype, detected in 10 (50%) of the 20 isolates identified (p = 0.05).</p> <p>Conclusions</p> <p>There was a gradual shift in prevailing adenovirus serotypes during the 17 month study period, from predominantly HAdV-3 to HAdV-2. If an adenovirus vaccine were to be broadly implemented, multiple serotypes should be included.</p> |
| first_indexed | 2024-12-18T14:01:33Z |
| format | Article |
| id | doaj.art-fe45e1f3d070477691e8154a68291c61 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-18T14:01:33Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-fe45e1f3d070477691e8154a68291c612022-12-21T21:05:20ZengBMCVirology Journal1743-422X2013-01-011011510.1186/1743-422X-10-15Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010Abbas Kaniza ZahraLombos ErnestoDuvvuri Venkata ROlsha RomyHiggins Rachel RGubbay Jonathan B<p>Abstract</p> <p>Background</p> <p>Certain adenovirus serotypes cause severe infections, especially in children. It is important to monitor temporal changes in serotypes causing clinical disease. The objective of this study was to document circulating respiratory adenovirus serotypes by sequencing adenovirus culture isolates from the Greater Toronto Area, Ontario, during December 2008 to April 2010.</p> <p>Methods</p> <p>Nucleic acid extraction was performed on 90 respiratory tract adenovirus culture isolates. PCR amplification was conducted with primers targeting the adenovirus hexon gene hypervariable region 7. Sanger sequencing and phylogenetic analyses were performed to determine serotype identities.</p> <p>Results</p> <p>Among 90 clinical respiratory isolates sequenced, eight different serotypes were identified. Serotype 3 (34, 38%), serotype 2 (30, 30%), and serotype 1 (14, 16%) isolates were most common; serotypes 5, 6, 11, 17 and 21 were also observed. Seventeen (50%) of the 34 HAdV-3 isolates were identified between December 2008 and February 2009, while none were identified from December 2009 to February 2010. Between December 2008 and April 2009, the two most common serotypes were HAdV-3 and HAdV-2, detected in 18 (53%) and 8 (24%) of the 34 cultures isolates, respectively. However, from December 2009 to April 2010, there was an increase in HAdV-2, which became the most prevalent serotype, detected in 10 (50%) of the 20 isolates identified (p = 0.05).</p> <p>Conclusions</p> <p>There was a gradual shift in prevailing adenovirus serotypes during the 17 month study period, from predominantly HAdV-3 to HAdV-2. If an adenovirus vaccine were to be broadly implemented, multiple serotypes should be included.</p>http://www.virologyj.com/content/10/1/15Human adenovirusesSerotypingMolecular typingPhylogeny |
| spellingShingle | Abbas Kaniza Zahra Lombos Ernesto Duvvuri Venkata R Olsha Romy Higgins Rachel R Gubbay Jonathan B Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 Virology Journal Human adenoviruses Serotyping Molecular typing Phylogeny |
| title | Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 |
| title_full | Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 |
| title_fullStr | Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 |
| title_full_unstemmed | Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 |
| title_short | Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010 |
| title_sort | temporal changes in respiratory adenovirus serotypes circulating in the greater toronto area ontario during december 2008 to april 2010 |
| topic | Human adenoviruses Serotyping Molecular typing Phylogeny |
| url | http://www.virologyj.com/content/10/1/15 |
| work_keys_str_mv | AT abbaskanizazahra temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 AT lombosernesto temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 AT duvvurivenkatar temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 AT olsharomy temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 AT higginsrachelr temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 AT gubbayjonathanb temporalchangesinrespiratoryadenovirusserotypescirculatinginthegreatertorontoareaontarioduringdecember2008toapril2010 |