Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice
<p>Abstract</p> <p>Background</p> <p>Stem/progenitor cell-based therapy has successfully been used as a novel therapeutic strategy for vascular diseases triggered by endothelial dysfunction. The aim of this study was to investigate the effects of mononuclear cell (MNC)...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/96 |
| _version_ | 1811297045260009472 |
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| author | Lima Leandro C F Porto Marcella L Campagnaro Bianca P Tonini Clarissa L Nogueira Breno V Pereira Thiago MC Vasquez Elisardo C Meyrelles Silvana S |
| author_facet | Lima Leandro C F Porto Marcella L Campagnaro Bianca P Tonini Clarissa L Nogueira Breno V Pereira Thiago MC Vasquez Elisardo C Meyrelles Silvana S |
| author_sort | Lima Leandro C F |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Stem/progenitor cell-based therapy has successfully been used as a novel therapeutic strategy for vascular diseases triggered by endothelial dysfunction. The aim of this study was to investigate the effects of mononuclear cell (MNC) therapy <it>in situ</it> on carotid cuff-induced occlusive thrombus in the apolipoprotein E knockout (apoE<sup>-/-</sup>) mouse.</p> <p>Methods</p> <p>Spleen-derived MNCs were isolated from green fluorescent protein (GFP)-transgenic mice for cell treatment. A cuff-induced thrombus model was produced by placing a nonconstrictive silastic collar around the left common carotid artery in 20-week-old female apoE<sup>-/-</sup> mice. After 10 days, the cuff was removed, and the animals received <it>in situ</it> MNCs (Cuff-MNC) or vehicle (Cuff-Vehicle) and were compared with sham-operated animals (Sham).</p> <p>Results</p> <p>The histological analysis showed that the MNC treatment reverted occlusive thrombus formation compared to the vehicle and the vessel lumen area to that observed in the Sham group (MNC, 50 ± 4; Vehicle, 20 ± 4; Sham, 55 ± 2 x10<sup>3</sup> μm<sup>2</sup>; p < 0.01). The animals that underwent the carotid cuff placement developed compensatory vessel enlargement, which was reduced by the MNC therapy. In addition, the treatment was able to reduce superoxide anion production, which likely contributed to the reduced apoptosis that was observed. Lastly, the immunofluorescence analysis revealed the presence of endothelial progenitor cells (EPCs) in the carotid endothelia of the apoE<sup>-/-</sup> mice.</p> <p>Conclusion</p> <p><it>In situ</it> short-term MNC therapy was able to revert cuff-induced occlusive thrombi in the carotid arteries of apoE<sup>-/-</sup> mice, possibly through the homing of EPCs, reduction of oxidative stress and decreased apoptosis.</p> |
| first_indexed | 2024-04-13T05:58:14Z |
| format | Article |
| id | doaj.art-fe47a73b4a7c4aba9c259c95c8420b52 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-13T05:58:14Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-fe47a73b4a7c4aba9c259c95c8420b522022-12-22T02:59:34ZengBMCLipids in Health and Disease1476-511X2012-07-011119610.1186/1476-511X-11-96Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient miceLima Leandro C FPorto Marcella LCampagnaro Bianca PTonini Clarissa LNogueira Breno VPereira Thiago MCVasquez Elisardo CMeyrelles Silvana S<p>Abstract</p> <p>Background</p> <p>Stem/progenitor cell-based therapy has successfully been used as a novel therapeutic strategy for vascular diseases triggered by endothelial dysfunction. The aim of this study was to investigate the effects of mononuclear cell (MNC) therapy <it>in situ</it> on carotid cuff-induced occlusive thrombus in the apolipoprotein E knockout (apoE<sup>-/-</sup>) mouse.</p> <p>Methods</p> <p>Spleen-derived MNCs were isolated from green fluorescent protein (GFP)-transgenic mice for cell treatment. A cuff-induced thrombus model was produced by placing a nonconstrictive silastic collar around the left common carotid artery in 20-week-old female apoE<sup>-/-</sup> mice. After 10 days, the cuff was removed, and the animals received <it>in situ</it> MNCs (Cuff-MNC) or vehicle (Cuff-Vehicle) and were compared with sham-operated animals (Sham).</p> <p>Results</p> <p>The histological analysis showed that the MNC treatment reverted occlusive thrombus formation compared to the vehicle and the vessel lumen area to that observed in the Sham group (MNC, 50 ± 4; Vehicle, 20 ± 4; Sham, 55 ± 2 x10<sup>3</sup> μm<sup>2</sup>; p < 0.01). The animals that underwent the carotid cuff placement developed compensatory vessel enlargement, which was reduced by the MNC therapy. In addition, the treatment was able to reduce superoxide anion production, which likely contributed to the reduced apoptosis that was observed. Lastly, the immunofluorescence analysis revealed the presence of endothelial progenitor cells (EPCs) in the carotid endothelia of the apoE<sup>-/-</sup> mice.</p> <p>Conclusion</p> <p><it>In situ</it> short-term MNC therapy was able to revert cuff-induced occlusive thrombi in the carotid arteries of apoE<sup>-/-</sup> mice, possibly through the homing of EPCs, reduction of oxidative stress and decreased apoptosis.</p>http://www.lipidworld.com/content/11/1/96ApoE<sup>-/-</sup>Mononuclear cellsThrombusCuff model |
| spellingShingle | Lima Leandro C F Porto Marcella L Campagnaro Bianca P Tonini Clarissa L Nogueira Breno V Pereira Thiago MC Vasquez Elisardo C Meyrelles Silvana S Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice Lipids in Health and Disease ApoE<sup>-/-</sup> Mononuclear cells Thrombus Cuff model |
| title | Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice |
| title_full | Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice |
| title_fullStr | Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice |
| title_full_unstemmed | Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice |
| title_short | Mononuclear cell therapy reverts cuff-induced thrombosis in apolipoprotein E-deficient mice |
| title_sort | mononuclear cell therapy reverts cuff induced thrombosis in apolipoprotein e deficient mice |
| topic | ApoE<sup>-/-</sup> Mononuclear cells Thrombus Cuff model |
| url | http://www.lipidworld.com/content/11/1/96 |
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