Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract
Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourie...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2013-06-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v5i2.p102-106 |
| _version_ | 1827092114514640896 |
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| author | Singaravelu Senthamilselvi Ponnuchamy Kumar Alagiyamanavalan Lakshmi Prabha Munisamy Govindaraju |
| author_facet | Singaravelu Senthamilselvi Ponnuchamy Kumar Alagiyamanavalan Lakshmi Prabha Munisamy Govindaraju |
| author_sort | Singaravelu Senthamilselvi |
| collection | DOAJ |
| description | Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Field Emission-Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Zeta potential measurements disclose the existence of silver nanoparticles. The phenolic compounds present in the aqueous leaves extract paves way for the possible reduction of silver to nano-silver. Electron microscopic studies revealed the average size of nanoparticles were 52 nm. The nanoparticles are highly stable by reaching a zeta potential value of −33.6 mV. Comparative antibacterial efficacy of silver nanoparticles was investigated by disk diffusion and microtitre broth dilution method against Escherichia coli. The result showed that silver nanoparticles are toxic to E. coli cells at higher concentration. Overall, we suggest microtitre broth dilution method is more reliable to determine the antimicrobial activity of silver nanoparticles than disk diffusion method. |
| first_indexed | 2024-03-11T21:46:08Z |
| format | Article |
| id | doaj.art-fe514a976237405ca56dd93309694ba4 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2025-03-20T06:07:03Z |
| publishDate | 2013-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-fe514a976237405ca56dd93309694ba42024-10-02T20:26:17ZengTsinghua University PressNano Biomedicine and Engineering2150-55782013-06-015210210610.5101/nbe.v5i2.p102-106Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf ExtractSingaravelu Senthamilselvi0Ponnuchamy Kumar1Alagiyamanavalan Lakshmi Prabha2Munisamy Govindaraju3Department of Plant Science, School of Life Sciences. Bharathidasan University, Tiruchirappalli – 620 024Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli – 620 024Department of Plant Science, School of Life Sciences. Bharathidasan University, Tiruchirappalli – 620 024Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli – 620 024Green nanotechnology involves the synthesis of nanoparticles without the involvement of toxic chemicals. The present study reports the green synthesis of silver nanoparticles from Annona squamosa leaf extract rapidly within 20 min. High throughput characterization such as UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Field Emission-Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Zeta potential measurements disclose the existence of silver nanoparticles. The phenolic compounds present in the aqueous leaves extract paves way for the possible reduction of silver to nano-silver. Electron microscopic studies revealed the average size of nanoparticles were 52 nm. The nanoparticles are highly stable by reaching a zeta potential value of −33.6 mV. Comparative antibacterial efficacy of silver nanoparticles was investigated by disk diffusion and microtitre broth dilution method against Escherichia coli. The result showed that silver nanoparticles are toxic to E. coli cells at higher concentration. Overall, we suggest microtitre broth dilution method is more reliable to determine the antimicrobial activity of silver nanoparticles than disk diffusion method.https://www.sciopen.com/article/10.5101/nbe.v5i2.p102-106green nanotechnologyannona squamosae. colidisk diffusionmicrotitre broth dilution method |
| spellingShingle | Singaravelu Senthamilselvi Ponnuchamy Kumar Alagiyamanavalan Lakshmi Prabha Munisamy Govindaraju Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract Nano Biomedicine and Engineering green nanotechnology annona squamosa e. coli disk diffusion microtitre broth dilution method |
| title | Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract |
| title_full | Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract |
| title_fullStr | Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract |
| title_full_unstemmed | Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract |
| title_short | Green Simplistic Biosynthesis of AntiBacterial Silver Nanoparticles Using Annona Squamosa Leaf Extract |
| title_sort | green simplistic biosynthesis of antibacterial silver nanoparticles using annona squamosa leaf extract |
| topic | green nanotechnology annona squamosa e. coli disk diffusion microtitre broth dilution method |
| url | https://www.sciopen.com/article/10.5101/nbe.v5i2.p102-106 |
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