Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection
Chitooligosaccharides (COSs) are a promising drug candidate and food ingredient because they are innately biocompatible, non-toxic, and non-allergenic to living tissues. Therefore, the impurities in COSs must be clearly elucidated and precisely determined. As for COSs, most analytical methods focus...
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MDPI AG
2017-02-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/15/2/51 |
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| author | Lidong Cao Xiuhuan Li Li Fan Li Zheng Miaomiao Wu Shanxue Zhang Qiliang Huang |
| author_facet | Lidong Cao Xiuhuan Li Li Fan Li Zheng Miaomiao Wu Shanxue Zhang Qiliang Huang |
| author_sort | Lidong Cao |
| collection | DOAJ |
| description | Chitooligosaccharides (COSs) are a promising drug candidate and food ingredient because they are innately biocompatible, non-toxic, and non-allergenic to living tissues. Therefore, the impurities in COSs must be clearly elucidated and precisely determined. As for COSs, most analytical methods focus on the determination of the average degrees of polymerization (DPs) and deacetylation (DD), as well as separation and analysis of the single COSs with different DPs. However, little is known about the concentrations of inorganic cations and anions in COSs. In the present study, an efficient and sensitive ion chromatography coupled with conductivity detection (IC-CD) for the determination of inorganic cations Na+, NH4+, K+, Mg2+, Ca2+, and chloride, acetate and lactate anions was developed. Detection limits were 0.01–0.05 μM for cations and 0.5–0.6 μM for anions. The linear range was 0.001–0.8 mM. The optimized analysis was carried out on IonPac CS12A and IonPac AS12A analytical column for cations and anions, respectively, using isocratic elution with 20 mM methanesulfonic acid and 4 mM sodium hydroxide aqueous solution as the mobile phase at a 1.0 mL/min flow rate. Quality parameters, including precision and accuracy, were fully validated and found to be satisfactory. The fully validated IC-CD method was readily applied for the quantification of various cations and anions in commercial COS technical concentrate. |
| first_indexed | 2024-12-10T07:51:28Z |
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| id | doaj.art-fe56441f0b8f4bd5b0f1773211fc0a94 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:51:28Z |
| publishDate | 2017-02-01 |
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| series | Marine Drugs |
| spelling | doaj.art-fe56441f0b8f4bd5b0f1773211fc0a942022-12-22T01:57:02ZengMDPI AGMarine Drugs1660-33972017-02-011525110.3390/md15020051md15020051Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity DetectionLidong Cao0Xiuhuan Li1Li Fan2Li Zheng3Miaomiao Wu4Shanxue Zhang5Qiliang Huang6Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, ChinaInstitute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, ChinaHainan Zhengye Zhongnong High-Tech Co., LTD., No. 25 Nansha Road, Haikou 570206, Hainan, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing 100193, ChinaChitooligosaccharides (COSs) are a promising drug candidate and food ingredient because they are innately biocompatible, non-toxic, and non-allergenic to living tissues. Therefore, the impurities in COSs must be clearly elucidated and precisely determined. As for COSs, most analytical methods focus on the determination of the average degrees of polymerization (DPs) and deacetylation (DD), as well as separation and analysis of the single COSs with different DPs. However, little is known about the concentrations of inorganic cations and anions in COSs. In the present study, an efficient and sensitive ion chromatography coupled with conductivity detection (IC-CD) for the determination of inorganic cations Na+, NH4+, K+, Mg2+, Ca2+, and chloride, acetate and lactate anions was developed. Detection limits were 0.01–0.05 μM for cations and 0.5–0.6 μM for anions. The linear range was 0.001–0.8 mM. The optimized analysis was carried out on IonPac CS12A and IonPac AS12A analytical column for cations and anions, respectively, using isocratic elution with 20 mM methanesulfonic acid and 4 mM sodium hydroxide aqueous solution as the mobile phase at a 1.0 mL/min flow rate. Quality parameters, including precision and accuracy, were fully validated and found to be satisfactory. The fully validated IC-CD method was readily applied for the quantification of various cations and anions in commercial COS technical concentrate.http://www.mdpi.com/1660-3397/15/2/51chitooligosaccharidesion chromatographyinorganic cations and anionsmethod validationquantification |
| spellingShingle | Lidong Cao Xiuhuan Li Li Fan Li Zheng Miaomiao Wu Shanxue Zhang Qiliang Huang Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection Marine Drugs chitooligosaccharides ion chromatography inorganic cations and anions method validation quantification |
| title | Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection |
| title_full | Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection |
| title_fullStr | Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection |
| title_full_unstemmed | Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection |
| title_short | Determination of Inorganic Cations and Anions in Chitooligosaccharides by Ion Chromatography with Conductivity Detection |
| title_sort | determination of inorganic cations and anions in chitooligosaccharides by ion chromatography with conductivity detection |
| topic | chitooligosaccharides ion chromatography inorganic cations and anions method validation quantification |
| url | http://www.mdpi.com/1660-3397/15/2/51 |
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