Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation
Background/purpose: Adhesive resin sealer systems are applied to seal root-canal systems more effectively through the formation of a resin impregnation layer. The purpose of this study was to investigate the effects of eluted components of the adhesive, Super-Bond sealer (SBS), on the proliferation...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-06-01
|
| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790212000347 |
| _version_ | 1819291566349484032 |
|---|---|
| author | Aya Kawasaki Yoshihiko Hayashi Kajiro Yanagiguchi Shizuka Yamada Minoru Syudo Kazunari Igawa Takeshi Ikeda Shisei Kubo Mamoru Fujiwara |
| author_facet | Aya Kawasaki Yoshihiko Hayashi Kajiro Yanagiguchi Shizuka Yamada Minoru Syudo Kazunari Igawa Takeshi Ikeda Shisei Kubo Mamoru Fujiwara |
| author_sort | Aya Kawasaki |
| collection | DOAJ |
| description | Background/purpose: Adhesive resin sealer systems are applied to seal root-canal systems more effectively through the formation of a resin impregnation layer. The purpose of this study was to investigate the effects of eluted components of the adhesive, Super-Bond sealer (SBS), on the proliferation of osteoblastic cells in vitro.
Materials and methods: The standard powder:liquid ratio according to the manufacturer's instructions was used to produce a cylindrical block of SBS (5 mm in diameter, 10 mm long) for this elution study. The resin block was placed on a 100-mm culture dish. Osteoblastic cells were seeded at a density of 4×106 in α-minimum essential medium containing 10% fetal bovine serum and cultured in a humidified incubator. After 3 days of culture with or without SBS, cells were retrieved and lysed according to the manufacturer's instructions. The cellular events induced by the eluted components from SBS were analyzed using an antibody assay for mitogen-activated protein kinases (MAPKs) and a 3-(4,5-dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the concentration of boron, a component of the catalyst, tri-n-butyl borane (TBB), was analyzed using inductively coupled plasma optical emission spectrometry.
Results: Expression of MAPKs increased after SBS application. The MTT assay indicated that TBB, one of the components of SBS, accelerated the proliferation of osteoblastic cells. Values of boron were 1.66±0.37 and 1.74±0.30 ppm in cells cultured with and without FBS, respectively.
Conclusions: The eluted components from SBS can increase the expression of some MAPKs related to osteoblastic cell proliferation and differentiation in vitro. Both the elution experiment and treatment of cell culture with SBS components indicated that the boron originating from TBB is likely to be responsible for activation of the proliferation of osteoblastic cells. |
| first_indexed | 2024-12-24T03:40:40Z |
| format | Article |
| id | doaj.art-fe58b5b52b5645fa9efff951af7e9c5f |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-24T03:40:40Z |
| publishDate | 2012-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-fe58b5b52b5645fa9efff951af7e9c5f2022-12-21T17:16:55ZengElsevierJournal of Dental Sciences1991-79022012-06-0172949810.1016/j.jds.2012.03.002Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferationAya Kawasaki0Yoshihiko Hayashi1Kajiro Yanagiguchi2Shizuka Yamada3Minoru Syudo4Kazunari Igawa5Takeshi Ikeda6Shisei Kubo7Mamoru Fujiwara8Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanSyudo Dental Clinic, Iizuka, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Clinical Education, Nagasaki University Hospital, Nagasaki, JapanDepartment of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanBackground/purpose: Adhesive resin sealer systems are applied to seal root-canal systems more effectively through the formation of a resin impregnation layer. The purpose of this study was to investigate the effects of eluted components of the adhesive, Super-Bond sealer (SBS), on the proliferation of osteoblastic cells in vitro. Materials and methods: The standard powder:liquid ratio according to the manufacturer's instructions was used to produce a cylindrical block of SBS (5 mm in diameter, 10 mm long) for this elution study. The resin block was placed on a 100-mm culture dish. Osteoblastic cells were seeded at a density of 4×106 in α-minimum essential medium containing 10% fetal bovine serum and cultured in a humidified incubator. After 3 days of culture with or without SBS, cells were retrieved and lysed according to the manufacturer's instructions. The cellular events induced by the eluted components from SBS were analyzed using an antibody assay for mitogen-activated protein kinases (MAPKs) and a 3-(4,5-dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the concentration of boron, a component of the catalyst, tri-n-butyl borane (TBB), was analyzed using inductively coupled plasma optical emission spectrometry. Results: Expression of MAPKs increased after SBS application. The MTT assay indicated that TBB, one of the components of SBS, accelerated the proliferation of osteoblastic cells. Values of boron were 1.66±0.37 and 1.74±0.30 ppm in cells cultured with and without FBS, respectively. Conclusions: The eluted components from SBS can increase the expression of some MAPKs related to osteoblastic cell proliferation and differentiation in vitro. Both the elution experiment and treatment of cell culture with SBS components indicated that the boron originating from TBB is likely to be responsible for activation of the proliferation of osteoblastic cells.http://www.sciencedirect.com/science/article/pii/S1991790212000347adhesive resin sealerboron concentrationMAPK expressionMTT assayosteoblastic cell proliferation |
| spellingShingle | Aya Kawasaki Yoshihiko Hayashi Kajiro Yanagiguchi Shizuka Yamada Minoru Syudo Kazunari Igawa Takeshi Ikeda Shisei Kubo Mamoru Fujiwara Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation Journal of Dental Sciences adhesive resin sealer boron concentration MAPK expression MTT assay osteoblastic cell proliferation |
| title | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation |
| title_full | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation |
| title_fullStr | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation |
| title_full_unstemmed | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation |
| title_short | Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation |
| title_sort | effects of eluted components from 4 meta mma tbb adhesive resin sealer on osteoblastic cell proliferation |
| topic | adhesive resin sealer boron concentration MAPK expression MTT assay osteoblastic cell proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S1991790212000347 |
| work_keys_str_mv | AT ayakawasaki effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT yoshihikohayashi effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT kajiroyanagiguchi effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT shizukayamada effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT minorusyudo effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT kazunariigawa effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT takeshiikeda effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT shiseikubo effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation AT mamorufujiwara effectsofelutedcomponentsfrom4metammatbbadhesiveresinsealeronosteoblasticcellproliferation |