Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery
The diversity of actinomycetes associated with the marine sponge <i>Coscinoderma mathewsi</i> collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as...
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/783 |
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| author | Yara I. Shamikh Aliaa A. El Shamy Yasser Gaber Usama Ramadan Abdelmohsen Hashem A. Madkour Hannes Horn Hossam M. Hassan Abeer H. Elmaidomy Dalal Hussien M. Alkhalifah Wael N. Hozzein |
| author_facet | Yara I. Shamikh Aliaa A. El Shamy Yasser Gaber Usama Ramadan Abdelmohsen Hashem A. Madkour Hannes Horn Hossam M. Hassan Abeer H. Elmaidomy Dalal Hussien M. Alkhalifah Wael N. Hozzein |
| author_sort | Yara I. Shamikh |
| collection | DOAJ |
| description | The diversity of actinomycetes associated with the marine sponge <i>Coscinoderma mathewsi</i> collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera <i>Micromonospora</i>, <i>Nocardia</i>, and <i>Gordonia</i>. Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete <i>Micromonospora</i> sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes (<i>Gordonia</i> sp. UA19 and <i>Nocardia</i> sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. <i>Micromonospora</i> sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested. |
| first_indexed | 2024-03-10T19:38:14Z |
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| id | doaj.art-fe5da79736c945839f5997f4dadae80b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:38:14Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fe5da79736c945839f5997f4dadae80b2023-11-20T01:31:13ZengMDPI AGMicroorganisms2076-26072020-05-018578310.3390/microorganisms8050783Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds DiscoveryYara I. Shamikh0Aliaa A. El Shamy1Yasser Gaber2Usama Ramadan Abdelmohsen3Hashem A. Madkour4Hannes Horn5Hossam M. Hassan6Abeer H. Elmaidomy7Dalal Hussien M. Alkhalifah8Wael N. Hozzein9Department of Microbiology and Immunology, Nahda University in Beni Suef, Beni-Suef 65211, EgyptDepartment of Microbiology and Public Health, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo 11785, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Marine and Environmental Geology, National Institute of Oceanography and Fisheries, Red Sea Branch, Hurghada 84511, EgyptIndependent Researcher, 69126 Heidelberg, GermanyDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptBiology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaBioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaThe diversity of actinomycetes associated with the marine sponge <i>Coscinoderma mathewsi</i> collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera <i>Micromonospora</i>, <i>Nocardia</i>, and <i>Gordonia</i>. Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete <i>Micromonospora</i> sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes (<i>Gordonia</i> sp. UA19 and <i>Nocardia</i> sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. <i>Micromonospora</i> sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested.https://www.mdpi.com/2076-2607/8/5/783spongesactinomycetescryptic<i>Micromonospora</i><i>Nocardia</i><i>Gordonia</i> |
| spellingShingle | Yara I. Shamikh Aliaa A. El Shamy Yasser Gaber Usama Ramadan Abdelmohsen Hashem A. Madkour Hannes Horn Hossam M. Hassan Abeer H. Elmaidomy Dalal Hussien M. Alkhalifah Wael N. Hozzein Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery Microorganisms sponges actinomycetes cryptic <i>Micromonospora</i> <i>Nocardia</i> <i>Gordonia</i> |
| title | Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
| title_full | Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
| title_fullStr | Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
| title_full_unstemmed | Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
| title_short | Actinomycetes from the Red Sea Sponge <i>Coscinoderma mathewsi</i>: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
| title_sort | actinomycetes from the red sea sponge i coscinoderma mathewsi i isolation diversity and potential for bioactive compounds discovery |
| topic | sponges actinomycetes cryptic <i>Micromonospora</i> <i>Nocardia</i> <i>Gordonia</i> |
| url | https://www.mdpi.com/2076-2607/8/5/783 |
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