Opium Induces Apoptosis in Jurkat Cells
Background: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2013-01-01
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| Series: | Addiction and Health |
| Online Access: | https://ahj.kmu.ac.ir/article_84590_344ab36ad68ca65dbc3141a9bff11766.pdf |
| _version_ | 1797681901737082880 |
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| author | Somayeh Igder Gholam-Reza Asadikaram Farzaneh Sheykholeslam Ahmad-Reza Sayadi Mehdi Mahmoodi Mohammad Kazemi Arababadi Mohammad Javad Rasaee |
| author_facet | Somayeh Igder Gholam-Reza Asadikaram Farzaneh Sheykholeslam Ahmad-Reza Sayadi Mehdi Mahmoodi Mohammad Kazemi Arababadi Mohammad Javad Rasaee |
| author_sort | Somayeh Igder |
| collection | DOAJ |
| description | Background: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric detection of surface phosphatidylserine. Findings: Significant differences in apoptosis of Jurkat cells were seen at 24 and 72 hours in different concentrations of opium (P < 0.05). After 72 hours, significant increase in necrosis of Jurkat cells was seen in opium concentration of 2.85 × 10-3 g/ml compared to cells without opium (control) (P < 0.05). Conclusion: These results showed that opium directly increases apoptosis and necrosis of T lymphocytes. This effect may play a role in immune dysfunction in opium addicts.Keywords: Opium, Apoptosis, Necrosis, Jurkat cells |
| first_indexed | 2024-03-11T23:51:44Z |
| format | Article |
| id | doaj.art-fe6014213daf49a5a2df8a000fefc3a5 |
| institution | Directory Open Access Journal |
| issn | 2008-4633 2008-8469 |
| language | English |
| last_indexed | 2024-03-11T23:51:44Z |
| publishDate | 2013-01-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Addiction and Health |
| spelling | doaj.art-fe6014213daf49a5a2df8a000fefc3a52023-09-19T06:53:06ZengKerman University of Medical SciencesAddiction and Health2008-46332008-84692013-01-0151-2273484590Opium Induces Apoptosis in Jurkat CellsSomayeh Igder0Gholam-Reza Asadikaram1Farzaneh Sheykholeslam2Ahmad-Reza Sayadi3Mehdi Mahmoodi4Mohammad Kazemi Arababadi5Mohammad Javad Rasaee6MSc Student, Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, IranProfessor, Physiology Research Center AND Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, IranAssistant Professor, Pasture Institute, Tehran, IranDepartment of Psychology, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, IranProfessor, Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, IranAssistant Professor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Kerman, IranProfessor, Department of Biotechnology, School of Medicine, Tarbiat Modares University, Tehran, IranBackground: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric detection of surface phosphatidylserine. Findings: Significant differences in apoptosis of Jurkat cells were seen at 24 and 72 hours in different concentrations of opium (P < 0.05). After 72 hours, significant increase in necrosis of Jurkat cells was seen in opium concentration of 2.85 × 10-3 g/ml compared to cells without opium (control) (P < 0.05). Conclusion: These results showed that opium directly increases apoptosis and necrosis of T lymphocytes. This effect may play a role in immune dysfunction in opium addicts.Keywords: Opium, Apoptosis, Necrosis, Jurkat cellshttps://ahj.kmu.ac.ir/article_84590_344ab36ad68ca65dbc3141a9bff11766.pdf |
| spellingShingle | Somayeh Igder Gholam-Reza Asadikaram Farzaneh Sheykholeslam Ahmad-Reza Sayadi Mehdi Mahmoodi Mohammad Kazemi Arababadi Mohammad Javad Rasaee Opium Induces Apoptosis in Jurkat Cells Addiction and Health |
| title | Opium Induces Apoptosis in Jurkat Cells |
| title_full | Opium Induces Apoptosis in Jurkat Cells |
| title_fullStr | Opium Induces Apoptosis in Jurkat Cells |
| title_full_unstemmed | Opium Induces Apoptosis in Jurkat Cells |
| title_short | Opium Induces Apoptosis in Jurkat Cells |
| title_sort | opium induces apoptosis in jurkat cells |
| url | https://ahj.kmu.ac.ir/article_84590_344ab36ad68ca65dbc3141a9bff11766.pdf |
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