Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice.
Weevils can devastate food legumes in developing countries, but genetically modified peas (Pisum sativum), chickpeas and cowpeas expressing the gene for alpha-amylase inhibitor-1 (αAI) from the common bean (Phaseolus vulgaris) are completely protected from weevil destruction. αAI is seed-specific, a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3541390?pdf=render |
| _version_ | 1811271819062149120 |
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| author | Rui-Yun Lee Daniela Reiner Gerhard Dekan Andrew E Moore T J V Higgins Michelle M Epstein |
| author_facet | Rui-Yun Lee Daniela Reiner Gerhard Dekan Andrew E Moore T J V Higgins Michelle M Epstein |
| author_sort | Rui-Yun Lee |
| collection | DOAJ |
| description | Weevils can devastate food legumes in developing countries, but genetically modified peas (Pisum sativum), chickpeas and cowpeas expressing the gene for alpha-amylase inhibitor-1 (αAI) from the common bean (Phaseolus vulgaris) are completely protected from weevil destruction. αAI is seed-specific, accumulated at high levels and undergoes post-translational modification as it traverses the seed endomembrane system. This modification was thought to be responsible for the reported allergenicity in mice of the transgenic pea but not the bean. Here, we observed that transgenic αAI peas, chickpeas and cowpeas as well as non-transgenic beans were all allergenic in BALB/c mice. Even consuming non-transgenic peas lacking αAI led to an anti-αAI response due to a cross-reactive response to pea lectin. Our data demonstrate that αAI transgenic peas are not more allergenic than beans or non-transgenic peas in mice. This study illustrates the importance of repeat experiments in independent laboratories and the potential for unexpected cross-reactive allergic responses upon consumption of plant products in mice. |
| first_indexed | 2024-04-12T22:29:01Z |
| format | Article |
| id | doaj.art-fe6171fca4024c238f6771c37d604747 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T22:29:01Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-fe6171fca4024c238f6771c37d6047472022-12-22T03:14:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5297210.1371/journal.pone.0052972Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice.Rui-Yun LeeDaniela ReinerGerhard DekanAndrew E MooreT J V HigginsMichelle M EpsteinWeevils can devastate food legumes in developing countries, but genetically modified peas (Pisum sativum), chickpeas and cowpeas expressing the gene for alpha-amylase inhibitor-1 (αAI) from the common bean (Phaseolus vulgaris) are completely protected from weevil destruction. αAI is seed-specific, accumulated at high levels and undergoes post-translational modification as it traverses the seed endomembrane system. This modification was thought to be responsible for the reported allergenicity in mice of the transgenic pea but not the bean. Here, we observed that transgenic αAI peas, chickpeas and cowpeas as well as non-transgenic beans were all allergenic in BALB/c mice. Even consuming non-transgenic peas lacking αAI led to an anti-αAI response due to a cross-reactive response to pea lectin. Our data demonstrate that αAI transgenic peas are not more allergenic than beans or non-transgenic peas in mice. This study illustrates the importance of repeat experiments in independent laboratories and the potential for unexpected cross-reactive allergic responses upon consumption of plant products in mice.http://europepmc.org/articles/PMC3541390?pdf=render |
| spellingShingle | Rui-Yun Lee Daniela Reiner Gerhard Dekan Andrew E Moore T J V Higgins Michelle M Epstein Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. PLoS ONE |
| title | Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. |
| title_full | Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. |
| title_fullStr | Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. |
| title_full_unstemmed | Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. |
| title_short | Genetically modified α-amylase inhibitor peas are not specifically allergenic in mice. |
| title_sort | genetically modified α amylase inhibitor peas are not specifically allergenic in mice |
| url | http://europepmc.org/articles/PMC3541390?pdf=render |
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