Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)
In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/16/2/1533/ |
| _version_ | 1818807424000196608 |
|---|---|
| author | Jian Liang Yi-Nan Zheng Yu-Fang He Xiao-Hua Li Zi Wang Li-Chun Zhao Wen-Cong Liu Wei Li |
| author_facet | Jian Liang Yi-Nan Zheng Yu-Fang He Xiao-Hua Li Zi Wang Li-Chun Zhao Wen-Cong Liu Wei Li |
| author_sort | Jian Liang |
| collection | DOAJ |
| description | In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum. |
| first_indexed | 2024-12-18T19:25:26Z |
| format | Article |
| id | doaj.art-fe64d766544241559d9ad236fb4676b3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T19:25:26Z |
| publishDate | 2011-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fe64d766544241559d9ad236fb4676b32022-12-21T20:55:53ZengMDPI AGMolecules1420-30492011-02-011621533154310.3390/molecules16021533Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)Jian LiangYi-Nan ZhengYu-Fang HeXiao-Hua LiZi WangLi-Chun ZhaoWen-Cong LiuWei LiIn the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.http://www.mdpi.com/1420-3049/16/2/1533/LC-DAD-ESI/MSsaikosaponinspressurized liquid extractionBupleurum falcatum |
| spellingShingle | Jian Liang Yi-Nan Zheng Yu-Fang He Xiao-Hua Li Zi Wang Li-Chun Zhao Wen-Cong Liu Wei Li Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) Molecules LC-DAD-ESI/MS saikosaponins pressurized liquid extraction Bupleurum falcatum |
| title | Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) |
| title_full | Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) |
| title_fullStr | Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) |
| title_full_unstemmed | Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) |
| title_short | Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) |
| title_sort | fast and sensitive lc dad esi ms method for analysis of saikosaponins c a and d from the roots of bupleurum falcatum sandaochaihu |
| topic | LC-DAD-ESI/MS saikosaponins pressurized liquid extraction Bupleurum falcatum |
| url | http://www.mdpi.com/1420-3049/16/2/1533/ |
| work_keys_str_mv | AT jianliang fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT yinanzheng fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT yufanghe fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT xiaohuali fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT ziwang fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT lichunzhao fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT wencongliu fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu AT weili fastandsensitivelcdadesimsmethodforanalysisofsaikosaponinscaanddfromtherootsofbupleurumfalcatumsandaochaihu |