Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
Abstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-91793-0 |
| _version_ | 1830081790403936256 |
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| author | Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda |
| author_facet | Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda |
| author_sort | Atsushi Yamanaka |
| collection | DOAJ |
| description | Abstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection. |
| first_indexed | 2024-12-14T15:47:07Z |
| format | Article |
| id | doaj.art-fe6708c7f6214a3189d92335ffda592b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:47:07Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fe6708c7f6214a3189d92335ffda592b2022-12-21T22:55:28ZengNature PortfolioScientific Reports2045-23222021-06-0111111110.1038/s41598-021-91793-0Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patientsAtsushi Yamanaka0Hisham Ahmed Imad1Weerapong Phumratanaprapin2Juthamas Phadungsombat3Eiji Konishi4Tatsuo Shioda5Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol UniversityMahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol UniversityBIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol UniversityMahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol UniversityAbstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection.https://doi.org/10.1038/s41598-021-91793-0 |
| spellingShingle | Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients Scientific Reports |
| title | Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| title_full | Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| title_fullStr | Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| title_full_unstemmed | Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| title_short | Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| title_sort | antibody dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients |
| url | https://doi.org/10.1038/s41598-021-91793-0 |
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