Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i>
<i>Verticillium dahliae</i> is one of the most destructive fungal pathogens, causing substantial economic losses in agriculture and forestry. The use of plant growth-promoting rhizobacteria (PGPR) is an effective and environmentally friendly strategy for controlling diseases caused by &l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/697 |
| _version_ | 1827596931593928704 |
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| author | Hang Ni Wei-Liang Kong Yu Zhang Xiao-Qin Wu |
| author_facet | Hang Ni Wei-Liang Kong Yu Zhang Xiao-Qin Wu |
| author_sort | Hang Ni |
| collection | DOAJ |
| description | <i>Verticillium dahliae</i> is one of the most destructive fungal pathogens, causing substantial economic losses in agriculture and forestry. The use of plant growth-promoting rhizobacteria (PGPR) is an effective and environmentally friendly strategy for controlling diseases caused by <i>V. dahliae</i>. In this study, 90 mm in diameter Petri plates were used to test the effect of volatile organic compounds (VOCs) produced by different concentrations of <i>Pseudomonas</i><i>aurantiaca</i> ST-TJ4 cells suspension on <i>V. dahliae</i> mycelia radial growth and biomass. The mycelial morphology was observed by using scanning electron microscopy. The conidia germination and microsclerotia formation of <i>V. dahliae</i> were evaluated. The VOCs with antifungal activity were collected by headspace solid-phase microextraction (SPME), and their components were analyzed by gas chromatography-mass spectrometry (GC-MS). The VOCs produced by strain ST-TJ4 significantly inhibited the growth of mycelium of <i>V. dahliae</i>. The morphology of the hyphae was rough and wrinkled when exposed to VOCs. The VOCs of strain ST-TJ4 have a significant inhibitory effect on <i>V. dahliae</i> conidia germination and microsclerotia formation. At the same time, the VOCs also reduce the expression of genes related to melanin synthesis in <i>V. dahliae</i>. In particular, the expression of the hydrophobin gene (<i>VDAG</i>-<i>02273</i>) was down-regulated the most, about 67-fold. The VOCs effectively alleviate the severity of cotton root disease. In the volatile profile of strain ST-TJ4, 2-undecanone and 1-nonanol assayed in the range 10–200 µL per plate revealed a significant inhibitory effect on <i>V. dahliae</i> mycelial radial growth. These compounds may be useful to devise new control strategies for control of Verticillium wilt disease caused by <i>V. dahliae</i>. |
| first_indexed | 2024-03-09T03:18:30Z |
| format | Article |
| id | doaj.art-fe67400ba43f4e7db23e337435febf26 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T03:18:30Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-fe67400ba43f4e7db23e337435febf262023-12-03T15:15:46ZengMDPI AGJournal of Fungi2309-608X2022-06-018769710.3390/jof8070697Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i>Hang Ni0Wei-Liang Kong1Yu Zhang2Xiao-Qin Wu3Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China<i>Verticillium dahliae</i> is one of the most destructive fungal pathogens, causing substantial economic losses in agriculture and forestry. The use of plant growth-promoting rhizobacteria (PGPR) is an effective and environmentally friendly strategy for controlling diseases caused by <i>V. dahliae</i>. In this study, 90 mm in diameter Petri plates were used to test the effect of volatile organic compounds (VOCs) produced by different concentrations of <i>Pseudomonas</i><i>aurantiaca</i> ST-TJ4 cells suspension on <i>V. dahliae</i> mycelia radial growth and biomass. The mycelial morphology was observed by using scanning electron microscopy. The conidia germination and microsclerotia formation of <i>V. dahliae</i> were evaluated. The VOCs with antifungal activity were collected by headspace solid-phase microextraction (SPME), and their components were analyzed by gas chromatography-mass spectrometry (GC-MS). The VOCs produced by strain ST-TJ4 significantly inhibited the growth of mycelium of <i>V. dahliae</i>. The morphology of the hyphae was rough and wrinkled when exposed to VOCs. The VOCs of strain ST-TJ4 have a significant inhibitory effect on <i>V. dahliae</i> conidia germination and microsclerotia formation. At the same time, the VOCs also reduce the expression of genes related to melanin synthesis in <i>V. dahliae</i>. In particular, the expression of the hydrophobin gene (<i>VDAG</i>-<i>02273</i>) was down-regulated the most, about 67-fold. The VOCs effectively alleviate the severity of cotton root disease. In the volatile profile of strain ST-TJ4, 2-undecanone and 1-nonanol assayed in the range 10–200 µL per plate revealed a significant inhibitory effect on <i>V. dahliae</i> mycelial radial growth. These compounds may be useful to devise new control strategies for control of Verticillium wilt disease caused by <i>V. dahliae</i>.https://www.mdpi.com/2309-608X/8/7/697<i>Pseudomonas aurantiaca</i>volatile organic compounds<i>Verticillium dahliae</i>melaninmicrosclerotia |
| spellingShingle | Hang Ni Wei-Liang Kong Yu Zhang Xiao-Qin Wu Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> Journal of Fungi <i>Pseudomonas aurantiaca</i> volatile organic compounds <i>Verticillium dahliae</i> melanin microsclerotia |
| title | Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> |
| title_full | Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> |
| title_fullStr | Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> |
| title_full_unstemmed | Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> |
| title_short | Effects of Volatile Organic Compounds Produced by <i>Pseudomonas aurantiaca</i> ST-TJ4 against <i>Verticillium dahliae</i> |
| title_sort | effects of volatile organic compounds produced by i pseudomonas aurantiaca i st tj4 against i verticillium dahliae i |
| topic | <i>Pseudomonas aurantiaca</i> volatile organic compounds <i>Verticillium dahliae</i> melanin microsclerotia |
| url | https://www.mdpi.com/2309-608X/8/7/697 |
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