Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia
In total, 46 <i>Microdochium</i> strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as <i>M. nivale</i> and &l...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/3/340 |
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| author | Tatiana Yu. Gagkaeva Aleksandra S. Orina Olga P. Gavrilova Nadezhda N. Gogina |
| author_facet | Tatiana Yu. Gagkaeva Aleksandra S. Orina Olga P. Gavrilova Nadezhda N. Gogina |
| author_sort | Tatiana Yu. Gagkaeva |
| collection | DOAJ |
| description | In total, 46 <i>Microdochium</i> strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as <i>M. nivale</i> and <i>M. majus</i>, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as <i>M. seminicola</i>, which, according to our findings, is the prevalent <i>Microdochium</i> species in this territory. This is the first record of <i>M. seminicola</i> in Russia. Attempts were made to distinguish between <i>Microdochium</i> species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of <i>Microdochium</i>. The predominance of three-septate macroconidia in <i>M. majus</i> was higher than that in <i>M. nivale</i> and typically exceeded 60% occurrence. Most <i>M. majus</i> and <i>M. nivale</i> strains formed walled protoperithecia on wheat stems. Only three strains of <i>M. majus</i> and one strain each of <i>M. nivale</i> and <i>M. seminicola</i> produced mature perithecia. The growth rate of <i>M. seminicola</i> strains was significantly lower on agar media at 5−25 °C than those of <i>M. majus</i> and <i>M. nivale</i> strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three <i>Microdochium</i> species did not produce any toxic metabolites. |
| first_indexed | 2024-12-19T22:52:36Z |
| format | Article |
| id | doaj.art-fe6763767d954aebbb8622225f43de9f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-19T22:52:36Z |
| publishDate | 2020-02-01 |
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| record_format | Article |
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| spelling | doaj.art-fe6763767d954aebbb8622225f43de9f2022-12-21T20:02:44ZengMDPI AGMicroorganisms2076-26072020-02-018334010.3390/microorganisms8030340microorganisms8030340Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in RussiaTatiana Yu. Gagkaeva0Aleksandra S. Orina1Olga P. Gavrilova2Nadezhda N. Gogina3All-Russian Institute of Plant Protection (VIZR), St.-Petersburg, 196608 Pushkin, RussiaAll-Russian Institute of Plant Protection (VIZR), St.-Petersburg, 196608 Pushkin, RussiaAll-Russian Institute of Plant Protection (VIZR), St.-Petersburg, 196608 Pushkin, RussiaAll-Russian Scientific Research and Technological Institute of Poultry, Sergiev Posad, 141311, Moscow region, RussiaIn total, 46 <i>Microdochium</i> strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as <i>M. nivale</i> and <i>M. majus</i>, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as <i>M. seminicola</i>, which, according to our findings, is the prevalent <i>Microdochium</i> species in this territory. This is the first record of <i>M. seminicola</i> in Russia. Attempts were made to distinguish between <i>Microdochium</i> species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of <i>Microdochium</i>. The predominance of three-septate macroconidia in <i>M. majus</i> was higher than that in <i>M. nivale</i> and typically exceeded 60% occurrence. Most <i>M. majus</i> and <i>M. nivale</i> strains formed walled protoperithecia on wheat stems. Only three strains of <i>M. majus</i> and one strain each of <i>M. nivale</i> and <i>M. seminicola</i> produced mature perithecia. The growth rate of <i>M. seminicola</i> strains was significantly lower on agar media at 5−25 °C than those of <i>M. majus</i> and <i>M. nivale</i> strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three <i>Microdochium</i> species did not produce any toxic metabolites.https://www.mdpi.com/2076-2607/8/3/340<i>microdochium nivale</i><i>m. majus</i><i>m. seminicola</i>seed-borne fungimorphologydnamycotoxins |
| spellingShingle | Tatiana Yu. Gagkaeva Aleksandra S. Orina Olga P. Gavrilova Nadezhda N. Gogina Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia Microorganisms <i>microdochium nivale</i> <i>m. majus</i> <i>m. seminicola</i> seed-borne fungi morphology dna mycotoxins |
| title | Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia |
| title_full | Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia |
| title_fullStr | Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia |
| title_full_unstemmed | Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia |
| title_short | Evidence of <i>Microdochium</i> Fungi Associated with Cereal Grains in Russia |
| title_sort | evidence of i microdochium i fungi associated with cereal grains in russia |
| topic | <i>microdochium nivale</i> <i>m. majus</i> <i>m. seminicola</i> seed-borne fungi morphology dna mycotoxins |
| url | https://www.mdpi.com/2076-2607/8/3/340 |
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