Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy
Abstract Background Cervical spondylotic myelopathy (CSM) is a clinically symptomatic condition due to spinal cord compression, leading to spinal cord dysfunction. Surgical decompression is the main treatment of CSM, but the mechanisms of axonal regeneration after surgical decompression are still fr...
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | BMC Neuroscience |
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| Online Access: | https://doi.org/10.1186/s12868-022-00727-7 |
| _version_ | 1811328690012815360 |
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| author | Peisheng Liu Xiaofeng Li Jing Liu Hengjia Zhang Zhitao You Jianfeng Zhang |
| author_facet | Peisheng Liu Xiaofeng Li Jing Liu Hengjia Zhang Zhitao You Jianfeng Zhang |
| author_sort | Peisheng Liu |
| collection | DOAJ |
| description | Abstract Background Cervical spondylotic myelopathy (CSM) is a clinically symptomatic condition due to spinal cord compression, leading to spinal cord dysfunction. Surgical decompression is the main treatment of CSM, but the mechanisms of axonal regeneration after surgical decompression are still fragmentary. Methods In a rat model of CSM, the cacna2d2 (α2δ2) expression levels in anterior horn of spinal cord were observed following compression and decompression by western blot and immunofluorescence. The expression levels of 5 hydroxytryptamine (5HT) and GAP43 were also analyzed by immunofluorescence. Furthermore, gabapentin intervention was performed for 4 weeks after decompression to analyze the changes of behaviors and anterior horn of spinal cords. Results Following decompression, the expression levels of α2δ2 in the anterior horn of spinal cord were decreased, but the expression levels of 5HT andGAP43 were increased. Compared with the vehicle treated rats, gabapentin treatment for 4 weeks ameliorated the behaviors of rats and improved the damaged anterior horn of spinal cord. Besides, inhibition of α2δ2 through gabapentin intervention enhanced the axonal regeneration in the anterior horn of damaged spinal cord. Conclusions Inhibition of α2δ2 could enhance axonal recovery in anterior horn of damaged spinal cord induced by CSM after surgical decompression, providing a potential method for promoting axon regeneration following surgery. |
| first_indexed | 2024-04-13T15:30:35Z |
| format | Article |
| id | doaj.art-fe67be3862c141a4a960fa46f3e4235f |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-04-13T15:30:35Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-fe67be3862c141a4a960fa46f3e4235f2022-12-22T02:41:24ZengBMCBMC Neuroscience1471-22022022-07-0123111210.1186/s12868-022-00727-7Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathyPeisheng Liu0Xiaofeng Li1Jing Liu2Hengjia Zhang3Zhitao You4Jianfeng Zhang5Department of Spinal Surgery, Yantaishan HospitalDepartment of Spinal Surgery, Yantaishan HospitalBasic Department, Yantai Vocational CollegeDepartment of Spinal Surgery, Yantaishan HospitalDepartment of Spinal Surgery, Yantaishan HospitalDepartment of Spinal Surgery, Yantaishan HospitalAbstract Background Cervical spondylotic myelopathy (CSM) is a clinically symptomatic condition due to spinal cord compression, leading to spinal cord dysfunction. Surgical decompression is the main treatment of CSM, but the mechanisms of axonal regeneration after surgical decompression are still fragmentary. Methods In a rat model of CSM, the cacna2d2 (α2δ2) expression levels in anterior horn of spinal cord were observed following compression and decompression by western blot and immunofluorescence. The expression levels of 5 hydroxytryptamine (5HT) and GAP43 were also analyzed by immunofluorescence. Furthermore, gabapentin intervention was performed for 4 weeks after decompression to analyze the changes of behaviors and anterior horn of spinal cords. Results Following decompression, the expression levels of α2δ2 in the anterior horn of spinal cord were decreased, but the expression levels of 5HT andGAP43 were increased. Compared with the vehicle treated rats, gabapentin treatment for 4 weeks ameliorated the behaviors of rats and improved the damaged anterior horn of spinal cord. Besides, inhibition of α2δ2 through gabapentin intervention enhanced the axonal regeneration in the anterior horn of damaged spinal cord. Conclusions Inhibition of α2δ2 could enhance axonal recovery in anterior horn of damaged spinal cord induced by CSM after surgical decompression, providing a potential method for promoting axon regeneration following surgery.https://doi.org/10.1186/s12868-022-00727-7Gabapentin5HTGAP43Iba-1Caspase-3 |
| spellingShingle | Peisheng Liu Xiaofeng Li Jing Liu Hengjia Zhang Zhitao You Jianfeng Zhang Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy BMC Neuroscience Gabapentin 5HT GAP43 Iba-1 Caspase-3 |
| title | Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| title_full | Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| title_fullStr | Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| title_full_unstemmed | Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| title_short | Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| title_sort | cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy |
| topic | Gabapentin 5HT GAP43 Iba-1 Caspase-3 |
| url | https://doi.org/10.1186/s12868-022-00727-7 |
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