Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea
Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alterna...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2024-04-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://ppjonline.org/upload/pdf/PPJ-OA-01-2024-0009.pdf |
| _version_ | 1797208896245334016 |
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| author | Hae-Jun Kim Se-Ryung Choi In-Sook Cho Rae-Dong Jeong |
| author_facet | Hae-Jun Kim Se-Ryung Choi In-Sook Cho Rae-Dong Jeong |
| author_sort | Hae-Jun Kim |
| collection | DOAJ |
| description | Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jeju-do (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry. |
| first_indexed | 2024-04-24T09:46:05Z |
| format | Article |
| id | doaj.art-fe68ba9e2c7c487ba5173259e5b746bd |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-24T09:46:05Z |
| publishDate | 2024-04-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-fe68ba9e2c7c487ba5173259e5b746bd2024-04-15T02:28:40ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802024-04-0140211512410.5423/PPJ.OA.01.2024.00092432Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in KoreaHae-Jun Kim0Se-Ryung Choi1In-Sook Cho2Rae-Dong Jeong3 Department of Applied Biology, Chonnam National University, Gwangju 61185, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Department of Applied Biology, Chonnam National University, Gwangju 61185, KoreaCitrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jeju-do (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.http://ppjonline.org/upload/pdf/PPJ-OA-01-2024-0009.pdfhigh-throughput sequencingidentificationsatsuma mandarinviruses |
| spellingShingle | Hae-Jun Kim Se-Ryung Choi In-Sook Cho Rae-Dong Jeong Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea The Plant Pathology Journal high-throughput sequencing identification satsuma mandarin viruses |
| title | Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea |
| title_full | Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea |
| title_fullStr | Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea |
| title_full_unstemmed | Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea |
| title_short | Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea |
| title_sort | viral metatranscriptomic analysis to reveal the diversity of viruses infecting satsuma mandarin citrus unshiu in korea |
| topic | high-throughput sequencing identification satsuma mandarin viruses |
| url | http://ppjonline.org/upload/pdf/PPJ-OA-01-2024-0009.pdf |
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