A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model
Airway hyperresponsiveness (AHR) has been proposed as a feature of pathogenesis of eosinophilic upper airway inflammation such as allergic rhinitis (AR). The measurement system for upper AHR (<sub>U</sub>AHR) in rodents is poorly developed, although measurements of nasal resistance have...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/7/252 |
| _version_ | 1818153108537081856 |
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| author | Dan Van Bui Akira Kanda Yoshiki Kobayashi Yoshiko Sakata Yumiko Kono Yoshiyuki Kamakura Takao Jinno Yasutaka Yun Kensuke Suzuki Shunsuke Sawada Mikiya Asako Akihiko Nakamura David Dombrowicz Keita Utsunomiya Tanigawa Noboru Koichi Tomoda Hiroshi Iwai |
| author_facet | Dan Van Bui Akira Kanda Yoshiki Kobayashi Yoshiko Sakata Yumiko Kono Yoshiyuki Kamakura Takao Jinno Yasutaka Yun Kensuke Suzuki Shunsuke Sawada Mikiya Asako Akihiko Nakamura David Dombrowicz Keita Utsunomiya Tanigawa Noboru Koichi Tomoda Hiroshi Iwai |
| author_sort | Dan Van Bui |
| collection | DOAJ |
| description | Airway hyperresponsiveness (AHR) has been proposed as a feature of pathogenesis of eosinophilic upper airway inflammation such as allergic rhinitis (AR). The measurement system for upper AHR (<sub>U</sub>AHR) in rodents is poorly developed, although measurements of nasal resistance have been reported. Here we assessed UAHR by direct measurement of swelling of the nasal mucosa induced by intranasal methacholine (MCh) using micro-computed tomography (micro-CT). Micro-CT analysis was performed in both naïve and ovalbumin-induced AR mice following intranasal administration of MCh. The nasal cavity was segmented into two-dimensional horizontal and axial planes, and the data for nasal mucosa were acquired for the region of interest threshold. Then, a ratio between the nasal mucosa area and nasal cavity area was calculated as nasal mucosa index. Using our novel method, nasal cavity structure was clearly identified on micro-CT, and dose-dependent increased swelling of the nasal mucosa was observed upon MCh treatment. Moreover, the nasal mucosa index was significantly increased in AR mice compared to controls following MCh treatment, while ovalbumin administration did not affect swelling of the nasal mucosa in either group. This UAHR following MCh treatment was completely reversed by pretreatment with glucocorticoids. This novel approach using micro-CT for investigating UAHR reflects a precise assessment system for swelling of the nasal mucosa following MCh treatment; it not only sheds light on the mechanism of AR but also contributes to the development of new therapeutic drugs in AR patients. |
| first_indexed | 2024-12-11T14:05:22Z |
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| id | doaj.art-fe69a947d7fd4cf2bb1c0807acc8a90c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T14:05:22Z |
| publishDate | 2019-06-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-fe69a947d7fd4cf2bb1c0807acc8a90c2022-12-22T01:03:42ZengMDPI AGBiomolecules2218-273X2019-06-019725210.3390/biom9070252biom9070252A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis ModelDan Van Bui0Akira Kanda1Yoshiki Kobayashi2Yoshiko Sakata3Yumiko Kono4Yoshiyuki Kamakura5Takao Jinno6Yasutaka Yun7Kensuke Suzuki8Shunsuke Sawada9Mikiya Asako10Akihiko Nakamura11David Dombrowicz12Keita Utsunomiya13Tanigawa Noboru14Koichi Tomoda15Hiroshi Iwai16Department of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanCentral Research Laboratory, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Radiology, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Information Systems, Faculty of Information Science and Technology, Osaka Institute of Technology, Hirakata 573-0196, JapanDepartment of Information Systems, Faculty of Information Science and Technology, Osaka Institute of Technology, Hirakata 573-0196, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanNakamura ENT Clinic, Sakai 591-8025, JapanUniversity of Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F-5900 Lille, FranceDepartment of Radiology, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Radiology, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, JapanAirway hyperresponsiveness (AHR) has been proposed as a feature of pathogenesis of eosinophilic upper airway inflammation such as allergic rhinitis (AR). The measurement system for upper AHR (<sub>U</sub>AHR) in rodents is poorly developed, although measurements of nasal resistance have been reported. Here we assessed UAHR by direct measurement of swelling of the nasal mucosa induced by intranasal methacholine (MCh) using micro-computed tomography (micro-CT). Micro-CT analysis was performed in both naïve and ovalbumin-induced AR mice following intranasal administration of MCh. The nasal cavity was segmented into two-dimensional horizontal and axial planes, and the data for nasal mucosa were acquired for the region of interest threshold. Then, a ratio between the nasal mucosa area and nasal cavity area was calculated as nasal mucosa index. Using our novel method, nasal cavity structure was clearly identified on micro-CT, and dose-dependent increased swelling of the nasal mucosa was observed upon MCh treatment. Moreover, the nasal mucosa index was significantly increased in AR mice compared to controls following MCh treatment, while ovalbumin administration did not affect swelling of the nasal mucosa in either group. This UAHR following MCh treatment was completely reversed by pretreatment with glucocorticoids. This novel approach using micro-CT for investigating UAHR reflects a precise assessment system for swelling of the nasal mucosa following MCh treatment; it not only sheds light on the mechanism of AR but also contributes to the development of new therapeutic drugs in AR patients.https://www.mdpi.com/2218-273X/9/7/252Allergic rhinitisairway hyperresponsiveness (AHR)eosinophilic airway inflammationmicro-CTand nasal resistance |
| spellingShingle | Dan Van Bui Akira Kanda Yoshiki Kobayashi Yoshiko Sakata Yumiko Kono Yoshiyuki Kamakura Takao Jinno Yasutaka Yun Kensuke Suzuki Shunsuke Sawada Mikiya Asako Akihiko Nakamura David Dombrowicz Keita Utsunomiya Tanigawa Noboru Koichi Tomoda Hiroshi Iwai A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model Biomolecules Allergic rhinitis airway hyperresponsiveness (AHR) eosinophilic airway inflammation micro-CT and nasal resistance |
| title | A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model |
| title_full | A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model |
| title_fullStr | A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model |
| title_full_unstemmed | A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model |
| title_short | A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model |
| title_sort | novel approach for investigating upper airway hyperresponsiveness using micro ct in eosinophilic upper airway inflammation such as allergic rhinitis model |
| topic | Allergic rhinitis airway hyperresponsiveness (AHR) eosinophilic airway inflammation micro-CT and nasal resistance |
| url | https://www.mdpi.com/2218-273X/9/7/252 |
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