Generation of sheep with defined FecB B and TBXT mutations and porcine blastocysts with KCNJ5 G151R/+ mutation using prime editing

Generation of sheep with defined FecB B and TBXT mutations and porcine blastocysts with KCNJ5 G151R/+ mutation using prime editing

Abstract Background Rewriting the genomes of living organisms has been a long-standing aim in the biological sciences. The revelation of the CRISPR/Cas9 technology has revolutionized the entire biological field. Since its emergence, this technology has been widely applied to induce gene knockouts, i...

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Bibliographic Details
Main Authors: Shiwei Zhou, Laura Johanna Lenk, Yawei Gao, Yuhui Wang, Xiaoe Zhao, Menghao Pan, Shuhong Huang, Kexin Sun, Peter Kalds, Qi Luo, Simon Lillico, Tad Sonstegard, Ute I. Scholl, Baohua Ma, Bjoern Petersen, Yulin Chen, Xiaolong Wang
Format: Article
Language:English
Published: BMC 2023-06-01
Series:BMC Genomics
Subjects:
Prime editing
Genetic improvement
Human disease modeling
Sheep
Pigs
Online Access:https://doi.org/10.1186/s12864-023-09409-y

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