Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
Abstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-12-01
|
| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.515 |
| _version_ | 1818837646069202944 |
|---|---|
| author | Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan |
| author_facet | Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan |
| author_sort | Qing Yuan |
| collection | DOAJ |
| description | Abstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression in the biological process, prognosis, and immune significance in glioma. Methods We analyzed the biological functions of SERPINA3 through data from the Chinese Glioma Genome Atlas databases. Differentially expressed genes and enrichment analysis were performed and correlations between SERPINA3 expression and immune cell infiltration were analyzed. Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. The correlations between the expression and clinical‐pathological parameters as well as other biomarkers were examined. Results Univariate and multivariate regression both indicated that the level of SERPINA3 transcript represented an independent prognostic factor. High levels of SERPINA3 correlated with poor survival in patients with glioma. Expression of SERPINA3 mRNA was observed positively correlated with MCM6, IGFBP2, and FKBP10. Enrichment analysis showed SERPINA3 mainly enriched in immune‐related terms and signaling pathways including MAPK, TNF, P53, PI3K‐Akt, nuclear factor‐κB. Immune infiltration analysis further declare the SERPINA3 expression negatively correlated with levels of Macrophages M1, native CD4+ T cell, monocytes, and Mast cell activated. And overexpression of SERPINA3 correlated with low CD4+ T cell infiltration in glioma tissues. Conclusions SERPINA3 may play a key role in the biological process of glioma cells especially in immune suppression activities. SERPINA3 may serve as an independent survival prediction factor in glioma patients. |
| first_indexed | 2024-12-19T03:25:48Z |
| format | Article |
| id | doaj.art-fe6d5b9851a54c37a27416b077301937 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-12-19T03:25:48Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-fe6d5b9851a54c37a27416b0773019372022-12-21T20:37:38ZengWileyImmunity, Inflammation and Disease2050-45272021-12-01941618163010.1002/iid3.515Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in gliomaQing Yuan0Song‐Quan Wang1Guang‐Tao Zhang2Jie He3Zhi‐Dan Liu4Ming‐Rong Wang5Hong‐Qing Cai6Jing‐Hai Wan7Department of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaState Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaAbstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression in the biological process, prognosis, and immune significance in glioma. Methods We analyzed the biological functions of SERPINA3 through data from the Chinese Glioma Genome Atlas databases. Differentially expressed genes and enrichment analysis were performed and correlations between SERPINA3 expression and immune cell infiltration were analyzed. Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. The correlations between the expression and clinical‐pathological parameters as well as other biomarkers were examined. Results Univariate and multivariate regression both indicated that the level of SERPINA3 transcript represented an independent prognostic factor. High levels of SERPINA3 correlated with poor survival in patients with glioma. Expression of SERPINA3 mRNA was observed positively correlated with MCM6, IGFBP2, and FKBP10. Enrichment analysis showed SERPINA3 mainly enriched in immune‐related terms and signaling pathways including MAPK, TNF, P53, PI3K‐Akt, nuclear factor‐κB. Immune infiltration analysis further declare the SERPINA3 expression negatively correlated with levels of Macrophages M1, native CD4+ T cell, monocytes, and Mast cell activated. And overexpression of SERPINA3 correlated with low CD4+ T cell infiltration in glioma tissues. Conclusions SERPINA3 may play a key role in the biological process of glioma cells especially in immune suppression activities. SERPINA3 may serve as an independent survival prediction factor in glioma patients.https://doi.org/10.1002/iid3.515gliomaimmune responseprognosisSERPINA3 |
| spellingShingle | Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma Immunity, Inflammation and Disease glioma immune response prognosis SERPINA3 |
| title | Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma |
| title_full | Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma |
| title_fullStr | Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma |
| title_full_unstemmed | Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma |
| title_short | Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma |
| title_sort | highly expressed of serpina3 indicated poor prognosis and involved in immune suppression in glioma |
| topic | glioma immune response prognosis SERPINA3 |
| url | https://doi.org/10.1002/iid3.515 |
| work_keys_str_mv | AT qingyuan highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT songquanwang highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT guangtaozhang highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT jiehe highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT zhidanliu highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT mingrongwang highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT hongqingcai highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma AT jinghaiwan highlyexpressedofserpina3indicatedpoorprognosisandinvolvedinimmunesuppressioninglioma |