Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein
protein is also including in the HBV genome targeting into the nucleus through modulating carboxyl residues by<br />phosphorylation. Nuclear localication Signal (NLS) in HBV core protein is inside the virion structure and it must be<br />unmasked in order to function, perhaps by phosphor...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2015-11-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7767 |
| _version_ | 1818990245490720768 |
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| author | Aris Haryanto Nastiti Wijayanti Michael Kann |
| author_facet | Aris Haryanto Nastiti Wijayanti Michael Kann |
| author_sort | Aris Haryanto |
| collection | DOAJ |
| description | protein is also including in the HBV genome targeting into the nucleus through modulating carboxyl residues by<br />phosphorylation. Nuclear localication Signal (NLS) in HBV core protein is inside the virion structure and it must be<br />unmasked in order to function, perhaps by phosphorylation. Phosphorylation of of HBV core protein in turn could<br />begin to alter capsid conformation. Staurosporine is a natural product originally isolated from bacterium<br />Streptomyces staurosporeus. Staurosporine was discovered to have biological activities ranging from anti-fungal to<br />anti-hypertensive. The interest in these activities resulted in a large investigative effort in chemistry and biology and<br />the discovery of the potential for anti-cancer treatment. The main biological activity of Staurosporine is the inhibition<br />of protein kinases through the prevention of ATP binding to the kinase. In the present study, we have studied the<br />intracellular localization of EGFP-Core fusion protein with triple HBV core and SV-40 nuclear localization signal at<br />its carboxyl terminal in presence and absence of Staurosporine. We also to study the effect of Staurosporine treatment<br />on the intracellular localization of EGFP-Core fusion protein in the hepatocyte cells line of HepG2 cell. Results<br />showed that effect of Staurosporine is prevent the nuclear localization of EGFP-Core fusion protein into nucleus<br />through an inhibition of the phosphorylation of core protein. Stauroporine also prevents cell division so that passive<br />trapping of core protein is inhibited. |
| first_indexed | 2024-12-20T19:51:18Z |
| format | Article |
| id | doaj.art-fe6ff126c891468daf42ed6aae38bc32 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-20T19:51:18Z |
| publishDate | 2015-11-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-fe6ff126c891468daf42ed6aae38bc322022-12-21T19:28:18ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011216494Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core ProteinAris HaryantoNastiti WijayantiMichael Kannprotein is also including in the HBV genome targeting into the nucleus through modulating carboxyl residues by<br />phosphorylation. Nuclear localication Signal (NLS) in HBV core protein is inside the virion structure and it must be<br />unmasked in order to function, perhaps by phosphorylation. Phosphorylation of of HBV core protein in turn could<br />begin to alter capsid conformation. Staurosporine is a natural product originally isolated from bacterium<br />Streptomyces staurosporeus. Staurosporine was discovered to have biological activities ranging from anti-fungal to<br />anti-hypertensive. The interest in these activities resulted in a large investigative effort in chemistry and biology and<br />the discovery of the potential for anti-cancer treatment. The main biological activity of Staurosporine is the inhibition<br />of protein kinases through the prevention of ATP binding to the kinase. In the present study, we have studied the<br />intracellular localization of EGFP-Core fusion protein with triple HBV core and SV-40 nuclear localization signal at<br />its carboxyl terminal in presence and absence of Staurosporine. We also to study the effect of Staurosporine treatment<br />on the intracellular localization of EGFP-Core fusion protein in the hepatocyte cells line of HepG2 cell. Results<br />showed that effect of Staurosporine is prevent the nuclear localization of EGFP-Core fusion protein into nucleus<br />through an inhibition of the phosphorylation of core protein. Stauroporine also prevents cell division so that passive<br />trapping of core protein is inhibited.http://journal.ugm.ac.id/ijbiotech/article/view/7767 |
| spellingShingle | Aris Haryanto Nastiti Wijayanti Michael Kann Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein Indonesian Journal of Biotechnology |
| title | Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein |
| title_full | Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein |
| title_fullStr | Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein |
| title_full_unstemmed | Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein |
| title_short | Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein |
| title_sort | effect of staurosporine on the intracellular localization of hepatitis b virus core protein |
| url | http://journal.ugm.ac.id/ijbiotech/article/view/7767 |
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