Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro
Abstract Objective Rhodococcus equi is an opportunistic pathogen that causes disease worldwide in young foals and immunocompromised humans. The interactions of R. equi with the host immune system have been described; however, most studies have been conducted using a few well-characterized strains. B...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-08-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4560-1 |
| _version_ | 1818267345980751872 |
|---|---|
| author | Adina R. Bujold Nicholas R. Lani Macarena G. Sanz |
| author_facet | Adina R. Bujold Nicholas R. Lani Macarena G. Sanz |
| author_sort | Adina R. Bujold |
| collection | DOAJ |
| description | Abstract Objective Rhodococcus equi is an opportunistic pathogen that causes disease worldwide in young foals and immunocompromised humans. The interactions of R. equi with the host immune system have been described; however, most studies have been conducted using a few well-characterized strains. Because biological differences between R. equi strains are not well characterized, it is unknown if experimental results will replicate when different strains are used. Therefore, our objective was to compare the growth and biofilm formation of low-passage-rate clinical isolates of R. equi to higher-passage-rate, commonly studied isolates to determine whether strain-to-strain variation exists. Results Twelve strains were used: 103+, ATCC 33701, UKVDL206 103S harboring a GFP-expressing plasmid, a plasmid-cured 33701 (higher-passage-rate) and seven low-passage clinical isolates. Generation time in liquid revealed fast, moderate-fast, moderate-slow, and slow-growing isolates. The higher-passage-rate isolates were among the moderate-slow growing strains. A strain’s rate of growth did not correspond to its ability to form biofilm nor to its colony size on solid media. Based on our results, care should be taken not to extrapolate in vitro work that may be conducted using different R. equi strains. Further work is needed to evaluate the effect that the observed differences may have on experimental results. |
| first_indexed | 2024-12-12T20:21:08Z |
| format | Article |
| id | doaj.art-fe715651743e4381acf5133cf4aadf5d |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-12T20:21:08Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-fe715651743e4381acf5133cf4aadf5d2022-12-22T00:13:15ZengBMCBMC Research Notes1756-05002019-08-011211610.1186/s13104-019-4560-1Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitroAdina R. Bujold0Nicholas R. Lani1Macarena G. Sanz2Department of Veterinary Clinical Sciences, Washington State UniversityDepartment of Veterinary Clinical Sciences, Washington State UniversityDepartment of Veterinary Clinical Sciences, Washington State UniversityAbstract Objective Rhodococcus equi is an opportunistic pathogen that causes disease worldwide in young foals and immunocompromised humans. The interactions of R. equi with the host immune system have been described; however, most studies have been conducted using a few well-characterized strains. Because biological differences between R. equi strains are not well characterized, it is unknown if experimental results will replicate when different strains are used. Therefore, our objective was to compare the growth and biofilm formation of low-passage-rate clinical isolates of R. equi to higher-passage-rate, commonly studied isolates to determine whether strain-to-strain variation exists. Results Twelve strains were used: 103+, ATCC 33701, UKVDL206 103S harboring a GFP-expressing plasmid, a plasmid-cured 33701 (higher-passage-rate) and seven low-passage clinical isolates. Generation time in liquid revealed fast, moderate-fast, moderate-slow, and slow-growing isolates. The higher-passage-rate isolates were among the moderate-slow growing strains. A strain’s rate of growth did not correspond to its ability to form biofilm nor to its colony size on solid media. Based on our results, care should be taken not to extrapolate in vitro work that may be conducted using different R. equi strains. Further work is needed to evaluate the effect that the observed differences may have on experimental results.http://link.springer.com/article/10.1186/s13104-019-4560-1Rhodococcus equiStrain-to-strain variationGrowthBiofilm |
| spellingShingle | Adina R. Bujold Nicholas R. Lani Macarena G. Sanz Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro BMC Research Notes Rhodococcus equi Strain-to-strain variation Growth Biofilm |
| title | Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro |
| title_full | Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro |
| title_fullStr | Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro |
| title_full_unstemmed | Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro |
| title_short | Strain-to-strain variation of Rhodococcus equi growth and biofilm formation in vitro |
| title_sort | strain to strain variation of rhodococcus equi growth and biofilm formation in vitro |
| topic | Rhodococcus equi Strain-to-strain variation Growth Biofilm |
| url | http://link.springer.com/article/10.1186/s13104-019-4560-1 |
| work_keys_str_mv | AT adinarbujold straintostrainvariationofrhodococcusequigrowthandbiofilmformationinvitro AT nicholasrlani straintostrainvariationofrhodococcusequigrowthandbiofilmformationinvitro AT macarenagsanz straintostrainvariationofrhodococcusequigrowthandbiofilmformationinvitro |