Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation
Accumulating evidences have reported that polysaccharide could alleviate colitis through the maintenance of intestinal homeostasis. In this study, an exopolysaccharide (EPS-K4) from Bacillus amyloliquefaciens was obtained via anionic-exchange chromatography which was with an average molecular weight...
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| Format: | Article |
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Elsevier
2020-12-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620304369 |
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| author | Jia-hua Kuang Yan-yan Huang Jin-shuang Hu Jia-jia Yu Qin-yu Zhou Dong-mei Liu |
| author_facet | Jia-hua Kuang Yan-yan Huang Jin-shuang Hu Jia-jia Yu Qin-yu Zhou Dong-mei Liu |
| author_sort | Jia-hua Kuang |
| collection | DOAJ |
| description | Accumulating evidences have reported that polysaccharide could alleviate colitis through the maintenance of intestinal homeostasis. In this study, an exopolysaccharide (EPS-K4) from Bacillus amyloliquefaciens was obtained via anionic-exchange chromatography which was with an average molecular weight of 10,067 Da and consisted of mannose, rhamnose, glucuronic acid, and glucose with a molar ratio of 40.09:23.65:11.42:17.68. Furthermore, administration of EPS-K4 relieved symptoms in colitis mice via gut microbiota modulation. The levels of pathogenic bacteria, such as Enterococcus and Escherichia-Shigella, were significantly suppressed. In contrast, the levels of Lactobacillus and intestinal microbiota diversity were increased. EPS-K4 could stimulate mucin secretion to maintain the integrity of the gut barrier and decrease the levels of virulent substances (endotoxin, diamine oxidase, and D-lactic acid) in serum. The pro-inflammatory cytokines (TNF-α) in serum were reduced, conversely, anti-inflammatory cytokines (IL-10) were increased. These findings indicated the possibility of EPS-K4 as a chemotherapy candidate for the prevention of colitis. |
| first_indexed | 2024-12-14T11:41:47Z |
| format | Article |
| id | doaj.art-fe73e58705274e37816ece827a4ee9dc |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T11:41:47Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-fe73e58705274e37816ece827a4ee9dc2022-12-21T23:02:48ZengElsevierJournal of Functional Foods1756-46462020-12-0175104212Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulationJia-hua Kuang0Yan-yan Huang1Jin-shuang Hu2Jia-jia Yu3Qin-yu Zhou4Dong-mei Liu5School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaCorresponding author.; School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, PR ChinaAccumulating evidences have reported that polysaccharide could alleviate colitis through the maintenance of intestinal homeostasis. In this study, an exopolysaccharide (EPS-K4) from Bacillus amyloliquefaciens was obtained via anionic-exchange chromatography which was with an average molecular weight of 10,067 Da and consisted of mannose, rhamnose, glucuronic acid, and glucose with a molar ratio of 40.09:23.65:11.42:17.68. Furthermore, administration of EPS-K4 relieved symptoms in colitis mice via gut microbiota modulation. The levels of pathogenic bacteria, such as Enterococcus and Escherichia-Shigella, were significantly suppressed. In contrast, the levels of Lactobacillus and intestinal microbiota diversity were increased. EPS-K4 could stimulate mucin secretion to maintain the integrity of the gut barrier and decrease the levels of virulent substances (endotoxin, diamine oxidase, and D-lactic acid) in serum. The pro-inflammatory cytokines (TNF-α) in serum were reduced, conversely, anti-inflammatory cytokines (IL-10) were increased. These findings indicated the possibility of EPS-K4 as a chemotherapy candidate for the prevention of colitis.http://www.sciencedirect.com/science/article/pii/S1756464620304369Bacillus amyloliquefaciensMicrobe polysaccharideColitisGut microbiomeMucinInflammation |
| spellingShingle | Jia-hua Kuang Yan-yan Huang Jin-shuang Hu Jia-jia Yu Qin-yu Zhou Dong-mei Liu Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation Journal of Functional Foods Bacillus amyloliquefaciens Microbe polysaccharide Colitis Gut microbiome Mucin Inflammation |
| title | Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation |
| title_full | Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation |
| title_fullStr | Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation |
| title_full_unstemmed | Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation |
| title_short | Exopolysaccharides from Bacillus amyloliquefaciens DMBA-K4 ameliorate dextran sodium sulfate-induced colitis via gut microbiota modulation |
| title_sort | exopolysaccharides from bacillus amyloliquefaciens dmba k4 ameliorate dextran sodium sulfate induced colitis via gut microbiota modulation |
| topic | Bacillus amyloliquefaciens Microbe polysaccharide Colitis Gut microbiome Mucin Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620304369 |
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