Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops
Protecting food crops from viral pathogens is a significant challenge for agriculture. An integral approach to genome-editing, known as CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR associated protein 9), is used to produce virus-resistant cultivars. The CRISPR/Ca...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/7/1264 |
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| author | Saleh Ahmed Shahriar M. Nazrul Islam Charles Ng Wai Chun Md. Abdur Rahim Narayan Chandra Paul Jasim Uddain Shafiquzzaman Siddiquee |
| author_facet | Saleh Ahmed Shahriar M. Nazrul Islam Charles Ng Wai Chun Md. Abdur Rahim Narayan Chandra Paul Jasim Uddain Shafiquzzaman Siddiquee |
| author_sort | Saleh Ahmed Shahriar |
| collection | DOAJ |
| description | Protecting food crops from viral pathogens is a significant challenge for agriculture. An integral approach to genome-editing, known as CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR associated protein 9), is used to produce virus-resistant cultivars. The CRISPR/Cas9 tool is an essential part of modern plant breeding due to its attractive features. Advances in plant breeding programs due to the incorporation of Cas9 have enabled the development of cultivars with heritable resistance to plant viruses. The resistance to viral DNA and RNA is generally provided using the Cas9 endonuclease and sgRNAs (single-guide RNAs) complex, targeting particular virus and host plant genomes by interrupting the viral cleavage or altering the plant host genome, thus reducing the replication ability of the virus. In this review, the CRISPR/Cas9 system and its application to staple food crops resistance against several destructive plant viruses are briefly described. We outline the key findings of recent Cas9 applications, including enhanced virus resistance, genetic mechanisms, research strategies, and challenges in economically important and globally cultivated food crop species. The research outcome of this emerging molecular technology can extend the development of agriculture and food security. We also describe the information gaps and address the unanswered concerns relating to plant viral resistance mediated by CRISPR/Cas9. |
| first_indexed | 2024-03-10T10:12:15Z |
| format | Article |
| id | doaj.art-fe73f24bbb824520a10de31cb009ae87 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T10:12:15Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-fe73f24bbb824520a10de31cb009ae872023-11-22T01:07:21ZengMDPI AGPlants2223-77472021-06-01107126410.3390/plants10071264Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food CropsSaleh Ahmed Shahriar0M. Nazrul Islam1Charles Ng Wai Chun2Md. Abdur Rahim3Narayan Chandra Paul4Jasim Uddain5Shafiquzzaman Siddiquee6School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaLaboratory of Plant Pathology and Microbiology, Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB R6M 1Y5, CanadaBioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang 11800, MalaysiaDepartment of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, KoreaDepartment of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshBiotechnology Research Institute, Universiti Malaysia Sabah, Jln UMS, Kota Kinabalu 88400, MalaysiaProtecting food crops from viral pathogens is a significant challenge for agriculture. An integral approach to genome-editing, known as CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR associated protein 9), is used to produce virus-resistant cultivars. The CRISPR/Cas9 tool is an essential part of modern plant breeding due to its attractive features. Advances in plant breeding programs due to the incorporation of Cas9 have enabled the development of cultivars with heritable resistance to plant viruses. The resistance to viral DNA and RNA is generally provided using the Cas9 endonuclease and sgRNAs (single-guide RNAs) complex, targeting particular virus and host plant genomes by interrupting the viral cleavage or altering the plant host genome, thus reducing the replication ability of the virus. In this review, the CRISPR/Cas9 system and its application to staple food crops resistance against several destructive plant viruses are briefly described. We outline the key findings of recent Cas9 applications, including enhanced virus resistance, genetic mechanisms, research strategies, and challenges in economically important and globally cultivated food crop species. The research outcome of this emerging molecular technology can extend the development of agriculture and food security. We also describe the information gaps and address the unanswered concerns relating to plant viral resistance mediated by CRISPR/Cas9.https://www.mdpi.com/2223-7747/10/7/1264CRISPR/Cas9food cropsplant viral diseasesresistance mechanisms and strategieschallenges |
| spellingShingle | Saleh Ahmed Shahriar M. Nazrul Islam Charles Ng Wai Chun Md. Abdur Rahim Narayan Chandra Paul Jasim Uddain Shafiquzzaman Siddiquee Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops Plants CRISPR/Cas9 food crops plant viral diseases resistance mechanisms and strategies challenges |
| title | Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops |
| title_full | Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops |
| title_fullStr | Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops |
| title_full_unstemmed | Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops |
| title_short | Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops |
| title_sort | control of plant viral diseases by crispr cas9 resistance mechanisms strategies and challenges in food crops |
| topic | CRISPR/Cas9 food crops plant viral diseases resistance mechanisms and strategies challenges |
| url | https://www.mdpi.com/2223-7747/10/7/1264 |
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