From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells
Current in vitro studies do not typically assess cellular impacts in relation to real-world atmospheric mixtures of gases. In this study, we set out to examine the feasibility of measuring biological responses at the level of gene expression in human lung cells upon direct exposures to air in the fi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2015-01-01
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| Series: | Environmental Health Insights |
| Online Access: | https://doi.org/10.4137/EHI.S15656 |
| _version_ | 1819173911630184448 |
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| author | William Vizuete Kenneth G. Sexton Hang Nguyen Lisa Smeester Kjersti Marie Aagaard Cynthia Shope Barry Lefer James H. Flynn Sergio Alvarez Mathew H. Erickson Rebecca C. Fry |
| author_facet | William Vizuete Kenneth G. Sexton Hang Nguyen Lisa Smeester Kjersti Marie Aagaard Cynthia Shope Barry Lefer James H. Flynn Sergio Alvarez Mathew H. Erickson Rebecca C. Fry |
| author_sort | William Vizuete |
| collection | DOAJ |
| description | Current in vitro studies do not typically assess cellular impacts in relation to real-world atmospheric mixtures of gases. In this study, we set out to examine the feasibility of measuring biological responses at the level of gene expression in human lung cells upon direct exposures to air in the field. This study describes the successful deployment of lung cells in the heavily industrialized Houston Ship Channel. By examining messenger RNA (mRNA) levels from exposed lung cells, we identified changes in genes that play a role as inflammatory responders in the cell. The results show anticipated responses from negative and positive controls, confirming the integrity of the experimental protocol and the successful deployment of the in vitro instrument. Furthermore, exposures to ambient conditions displayed robust changes in gene expression. These results demonstrate a methodology that can produce gas-phase toxicity data in the field. |
| first_indexed | 2024-12-22T20:30:36Z |
| format | Article |
| id | doaj.art-fe748d8535784f7e9dda6e23ea87680c |
| institution | Directory Open Access Journal |
| issn | 1178-6302 |
| language | English |
| last_indexed | 2024-12-22T20:30:36Z |
| publishDate | 2015-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Environmental Health Insights |
| spelling | doaj.art-fe748d8535784f7e9dda6e23ea87680c2022-12-21T18:13:36ZengSAGE PublishingEnvironmental Health Insights1178-63022015-01-019s410.4137/EHI.S15656From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung CellsWilliam Vizuete0Kenneth G. Sexton1Hang Nguyen2Lisa Smeester3Kjersti Marie Aagaard4Cynthia Shope5Barry Lefer6James H. Flynn7Sergio Alvarez8Mathew H. Erickson9Rebecca C. Fry10Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.Division of Maternal Fetal Medicine, Baylor College of Medicine, Houston, TX, USA.Division of Maternal Fetal Medicine, Baylor College of Medicine, Houston, TX, USA.Department of Earth and Atmospheric Sciences, University of Houston, Houston, TX, USA.Department of Earth and Atmospheric Sciences, University of Houston, Houston, TX, USA.Department of Earth and Atmospheric Sciences, University of Houston, Houston, TX, USA.Department of Earth and Atmospheric Sciences, University of Houston, Houston, TX, USA.Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.Current in vitro studies do not typically assess cellular impacts in relation to real-world atmospheric mixtures of gases. In this study, we set out to examine the feasibility of measuring biological responses at the level of gene expression in human lung cells upon direct exposures to air in the field. This study describes the successful deployment of lung cells in the heavily industrialized Houston Ship Channel. By examining messenger RNA (mRNA) levels from exposed lung cells, we identified changes in genes that play a role as inflammatory responders in the cell. The results show anticipated responses from negative and positive controls, confirming the integrity of the experimental protocol and the successful deployment of the in vitro instrument. Furthermore, exposures to ambient conditions displayed robust changes in gene expression. These results demonstrate a methodology that can produce gas-phase toxicity data in the field.https://doi.org/10.4137/EHI.S15656 |
| spellingShingle | William Vizuete Kenneth G. Sexton Hang Nguyen Lisa Smeester Kjersti Marie Aagaard Cynthia Shope Barry Lefer James H. Flynn Sergio Alvarez Mathew H. Erickson Rebecca C. Fry From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells Environmental Health Insights |
| title | From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells |
| title_full | From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells |
| title_fullStr | From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells |
| title_full_unstemmed | From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells |
| title_short | From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells |
| title_sort | from the field to the laboratory air pollutant induced genomic effects in lung cells |
| url | https://doi.org/10.4137/EHI.S15656 |
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