Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress
High temperature in summer is an unfavorable factor for passion fruit (Passiflora edulis), which can lead to restricted growth, short flowering period, few flower buds, low fruit setting rate, severe fruit drop, and more deformed fruit. To explore the molecular physiology mechanism of passion fruit...
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PeerJ Inc.
2023-02-01
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| Online Access: | https://peerj.com/articles/14839.pdf |
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| author | Hongli Wang Jiucheng Zhao Miao Lai Yingqing Zhang Wenwu Qiu Yanyan Li Hailian Tu Qichang Ling Xinfeng Fu |
| author_facet | Hongli Wang Jiucheng Zhao Miao Lai Yingqing Zhang Wenwu Qiu Yanyan Li Hailian Tu Qichang Ling Xinfeng Fu |
| author_sort | Hongli Wang |
| collection | DOAJ |
| description | High temperature in summer is an unfavorable factor for passion fruit (Passiflora edulis), which can lead to restricted growth, short flowering period, few flower buds, low fruit setting rate, severe fruit drop, and more deformed fruit. To explore the molecular physiology mechanism of passion fruit responding to high-temperature stress, we use ‘Zhuangxiang Mibao’, a hybrid passion fruit cultivar, as the test material. Several physiological indicators were measured and compared between high-temperature (average temperature 38 °C) and normal temperature (average temperature 25 °C) conditions, including photosynthesis, chlorophyll fluorescence parameters, peroxidase activity (POD), superoxide dismutase activity (SOD) and malondialdehyde content. We performed RNA-seq analysis combined with biochemistry experiment to investigate the gene and molecular pathways that respond to high-temperature stress. The results showed that some physiological indicators in the high-temperature group, including the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and the maximum chemical quantum yield of photosystemII (PSII), were significantly lower than those of the control group. Malondialdehyde content was substantially higher than the control group, while superoxide dismutase and superoxide dismutase activities decreased to different degrees. Transcriptome sequencing analysis showed that 140 genes were up-regulated and 75 genes were down-regulated under high-temperature stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis of differentially expressed genes revealed many metabolic pathways related to high-temperature stress. Further investigation revealed that 30 genes might be related to high-temperature stress, such as chlorophyllide a oxygenase (CAO), glutathione (GSH), WRKY transcription factors (WRKY), and heat shock protein (HSP), which have also been reported in other species. The results of real-time fluorescence quantitative PCR and RNA-seq of randomly selected ten genes are consistent, which suggests that the transcriptome sequencing results were reliable. Our study provides a theoretical basis for the mechanism of passion fruit response to high-temperature stress. Also, it gives a theoretical basis for the subsequent breeding of new heat-resistant passion fruit varieties. |
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| language | English |
| last_indexed | 2024-03-09T05:15:33Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-fe752e5ab41e41f7be525783a2e761f32023-12-03T12:46:17ZengPeerJ Inc.PeerJ2167-83592023-02-0111e1483910.7717/peerj.14839Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stressHongli Wang0Jiucheng Zhao1Miao Lai2Yingqing Zhang3Wenwu Qiu4Yanyan Li5Hailian Tu6Qichang Ling7Xinfeng Fu8Qinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaInstitute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaQinzhou Branch of Guangxi Academy of Agricultural Sciences/Qinzhou Institute of Agricultural Sciences, Qinzhou, ChinaHigh temperature in summer is an unfavorable factor for passion fruit (Passiflora edulis), which can lead to restricted growth, short flowering period, few flower buds, low fruit setting rate, severe fruit drop, and more deformed fruit. To explore the molecular physiology mechanism of passion fruit responding to high-temperature stress, we use ‘Zhuangxiang Mibao’, a hybrid passion fruit cultivar, as the test material. Several physiological indicators were measured and compared between high-temperature (average temperature 38 °C) and normal temperature (average temperature 25 °C) conditions, including photosynthesis, chlorophyll fluorescence parameters, peroxidase activity (POD), superoxide dismutase activity (SOD) and malondialdehyde content. We performed RNA-seq analysis combined with biochemistry experiment to investigate the gene and molecular pathways that respond to high-temperature stress. The results showed that some physiological indicators in the high-temperature group, including the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and the maximum chemical quantum yield of photosystemII (PSII), were significantly lower than those of the control group. Malondialdehyde content was substantially higher than the control group, while superoxide dismutase and superoxide dismutase activities decreased to different degrees. Transcriptome sequencing analysis showed that 140 genes were up-regulated and 75 genes were down-regulated under high-temperature stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis of differentially expressed genes revealed many metabolic pathways related to high-temperature stress. Further investigation revealed that 30 genes might be related to high-temperature stress, such as chlorophyllide a oxygenase (CAO), glutathione (GSH), WRKY transcription factors (WRKY), and heat shock protein (HSP), which have also been reported in other species. The results of real-time fluorescence quantitative PCR and RNA-seq of randomly selected ten genes are consistent, which suggests that the transcriptome sequencing results were reliable. Our study provides a theoretical basis for the mechanism of passion fruit response to high-temperature stress. Also, it gives a theoretical basis for the subsequent breeding of new heat-resistant passion fruit varieties.https://peerj.com/articles/14839.pdfPassion fruitHigh-temperature stressChlorophyll fluorescenceRNA-seqDifferential expression genePathway |
| spellingShingle | Hongli Wang Jiucheng Zhao Miao Lai Yingqing Zhang Wenwu Qiu Yanyan Li Hailian Tu Qichang Ling Xinfeng Fu Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress PeerJ Passion fruit High-temperature stress Chlorophyll fluorescence RNA-seq Differential expression gene Pathway |
| title | Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress |
| title_full | Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress |
| title_fullStr | Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress |
| title_full_unstemmed | Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress |
| title_short | Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress |
| title_sort | differential gene expression analysis and physiological response characteristics of passion fruit passiflora edulis buds under high temperature stress |
| topic | Passion fruit High-temperature stress Chlorophyll fluorescence RNA-seq Differential expression gene Pathway |
| url | https://peerj.com/articles/14839.pdf |
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