Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes)
‘Wreckfish’ a collective of species belonging to the family Polyprionidae, are an important commercial fishery and have significant aquaculture potential. Until now, genomic or transcriptomic information for any species within the genus Polyprion has either remained unpublished o...
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MDPI AG
2019-02-01
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| Series: | Fishes |
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| Online Access: | http://www.mdpi.com/2410-3888/4/1/16 |
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| author | Matthew J Wylie Jane E Symonds Alvin N Setiawan Glen W Irvine Hui Liu Abigail Elizur P Mark Lokman |
| author_facet | Matthew J Wylie Jane E Symonds Alvin N Setiawan Glen W Irvine Hui Liu Abigail Elizur P Mark Lokman |
| author_sort | Matthew J Wylie |
| collection | DOAJ |
| description | ‘Wreckfish’ a collective of species belonging to the family Polyprionidae, are an important commercial fishery and have significant aquaculture potential. Until now, genomic or transcriptomic information for any species within the genus Polyprion has either remained unpublished or is non-existent. Using Illumina HiSeq, we compared the transcriptomes of hāpuku (Polyprion oxygeneios) ovaries to explore developmental stage-specific variations underlying their reproductive physiology. We sought to identify differentially expressed genes and the associated shifts in biological pathways between previtellogenic and early vitellogenic ovaries. Ovarian tissue was repeatedly biopsied by gonopore cannulation from the same females (n = 3) throughout oogenesis. Reproductive status of initial biopsies was confirmed as being previtellogenic and that in biopsies collected eight weeks later as early vitellogenic. A de novo hāpuku transcriptome was assembled (146,189 transcripts) from RNA-Seq data without a reference genome. On average, each tissue sample contained 17.5 million trimmed reads. Gene annotation was 80% when using BLASTX against Genbank Non Redundant database. Fifty-three transcripts were differentially expressed within the FDR of 0.05 when previtellogenic and early vitellogenic ovaries were compared; this reduced to 35 differentially expressed genes when transcript duplications were pooled. Among these were genes tentatively associated with the electron transport chain, lipid metabolism, steroidogenesis and mineral/solute transportation. These data provide a snap-shot into stage-specific physiological events during oogenesis in the ovary of a teleost and an extensive molecular resource for research on species in the Genus Polyprion. |
| first_indexed | 2024-12-12T19:04:33Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-12-12T19:04:33Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-fe765cf7313441b7b9733e8dd5e004e92022-12-22T00:14:59ZengMDPI AGFishes2410-38882019-02-014116010.3390/fishes4010016fishes4010016Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes)Matthew J Wylie0Jane E Symonds1Alvin N Setiawan2Glen W Irvine3Hui Liu4Abigail Elizur5P Mark Lokman6Department of Zoology, University of Otago, PO Box 56, Dunedin 9054, New ZealandNational Institute of Water and Atmospheric Research, Northland Marine Research Centre, PO Box 147, Ruakaka 0151, New ZealandNational Institute of Water and Atmospheric Research, Northland Marine Research Centre, PO Box 147, Ruakaka 0151, New ZealandNational Institute of Water and Atmospheric Research, Northland Marine Research Centre, PO Box 147, Ruakaka 0151, New ZealandDepartment of Anatomy, University of Otago, 270 Great King Street, Dunedin 9016, New ZealandGeneCology Research Centre, University of the Sunshine Coast, Queensland 4556, AustraliaDepartment of Zoology, University of Otago, PO Box 56, Dunedin 9054, New Zealand‘Wreckfish’ a collective of species belonging to the family Polyprionidae, are an important commercial fishery and have significant aquaculture potential. Until now, genomic or transcriptomic information for any species within the genus Polyprion has either remained unpublished or is non-existent. Using Illumina HiSeq, we compared the transcriptomes of hāpuku (Polyprion oxygeneios) ovaries to explore developmental stage-specific variations underlying their reproductive physiology. We sought to identify differentially expressed genes and the associated shifts in biological pathways between previtellogenic and early vitellogenic ovaries. Ovarian tissue was repeatedly biopsied by gonopore cannulation from the same females (n = 3) throughout oogenesis. Reproductive status of initial biopsies was confirmed as being previtellogenic and that in biopsies collected eight weeks later as early vitellogenic. A de novo hāpuku transcriptome was assembled (146,189 transcripts) from RNA-Seq data without a reference genome. On average, each tissue sample contained 17.5 million trimmed reads. Gene annotation was 80% when using BLASTX against Genbank Non Redundant database. Fifty-three transcripts were differentially expressed within the FDR of 0.05 when previtellogenic and early vitellogenic ovaries were compared; this reduced to 35 differentially expressed genes when transcript duplications were pooled. Among these were genes tentatively associated with the electron transport chain, lipid metabolism, steroidogenesis and mineral/solute transportation. These data provide a snap-shot into stage-specific physiological events during oogenesis in the ovary of a teleost and an extensive molecular resource for research on species in the Genus Polyprion.http://www.mdpi.com/2410-3888/4/1/16PolypriontranscriptomevitellogenesisRNA-Seqoogenesishāpukude novosteroidlipid |
| spellingShingle | Matthew J Wylie Jane E Symonds Alvin N Setiawan Glen W Irvine Hui Liu Abigail Elizur P Mark Lokman Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) Fishes Polyprion transcriptome vitellogenesis RNA-Seq oogenesis hāpuku de novo steroid lipid |
| title | Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) |
| title_full | Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) |
| title_fullStr | Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) |
| title_full_unstemmed | Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) |
| title_short | Transcriptomic Changes during Previtellogenic and Vitellogenic Stages of Ovarian Development in Wreckfish (Hāpuku), Polyprion oxygeneios (Perciformes) |
| title_sort | transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish hapuku polyprion oxygeneios perciformes |
| topic | Polyprion transcriptome vitellogenesis RNA-Seq oogenesis hāpuku de novo steroid lipid |
| url | http://www.mdpi.com/2410-3888/4/1/16 |
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