Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
Abstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-021-08957-4 |
| _version_ | 1819003275745165312 |
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| author | Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li |
| author_facet | Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li |
| author_sort | Qunhao Zheng |
| collection | DOAJ |
| description | Abstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the prognostic value and potential biological function of the SEC61G gene in LUAD. Methods Public datasets were used for SEC61G expression analyses. The prognostic value of SEC61G in LUAD was investigated using the Kaplan–Meier survival and Cox analyses. The correlation between the methylation level of SEC61G and its mRNA expression was evaluated via cBioPortal. Additionally, MethSurv was used to determine the prognostic value of the SEC61G methylation levels in LUAD. Functional enrichment analysis was conducted to explore the potential mechanism of SEC61G. Also, single sample GSEA (ssGSEA) and TIMER online tool were applied to identify the correlation between SEC61G and immune filtration. Furthermore, cell functional experiments were conducted to verify the biological behavior of SEC61G in lung adenocarcinoma cells (LAC). Results SEC61G was upregulated in pan-cancers, including LUAD. High SEC61G expression was significantly correlated with worse prognosis in LUAD patients. Multivariate analysis demonstrated that high SEC61G expression was an independent prognostic factor in the TCGA cohort. (HR = 1.760 95% CI: 1.297–2.388, p < 0.001). The methylation level of SEC61G negatively correlated with the SEC61G expression (R = − 0.290, p < 0.001), and patients with low SEC61G methylation had worse overall survival. (p = 0.0014). Proliferation-associated terms such as cell cycle and cell division were significantly enriched in GO and KEGG analysis. Vitro experiments demonstrated that knockdown of SEC61G resulted in decreased cell proliferation, invasion and facilitated apoptosis in LAC. GSEA analysis found that SEC61G expression was associated with the E2F targets. Moreover, SEC61G expression was negatively correlated with the immune cell infiltration including CD4+ T cell, CD8+ T cell, B cell, macrophage, neutrophil, and dendritic cell. Conclusion Our study indicated that overexpression of SEC61G was significantly associated with poor prognosis of LUAD patients and the malignant phenotypes of LUAD cells, suggesting that it could be a novel prognostic biomarker and potential therapeutic target of LUAD. |
| first_indexed | 2024-12-20T23:18:25Z |
| format | Article |
| id | doaj.art-fe7a6cf687e24966832fd6bf854892c5 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-20T23:18:25Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-fe7a6cf687e24966832fd6bf854892c52022-12-21T19:23:35ZengBMCBMC Cancer1471-24072021-11-0121111810.1186/s12885-021-08957-4Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validationQunhao Zheng0Zhiping Wang1Mengyan Zhang2Yilin Yu3Rui Chen4Tianzhu Lu5Lingyun Liu6Jiayu Ma7Tianxiu Liu8Hongying Zheng9Hui Li10Jiancheng Li11Fujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalJiangxi Cancer Hospital of Nanchang UniversityJiangxi Cancer Hospital of Nanchang UniversityFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalFujian Medical University Cancer Hospital, Fujian Cancer HospitalAbstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the prognostic value and potential biological function of the SEC61G gene in LUAD. Methods Public datasets were used for SEC61G expression analyses. The prognostic value of SEC61G in LUAD was investigated using the Kaplan–Meier survival and Cox analyses. The correlation between the methylation level of SEC61G and its mRNA expression was evaluated via cBioPortal. Additionally, MethSurv was used to determine the prognostic value of the SEC61G methylation levels in LUAD. Functional enrichment analysis was conducted to explore the potential mechanism of SEC61G. Also, single sample GSEA (ssGSEA) and TIMER online tool were applied to identify the correlation between SEC61G and immune filtration. Furthermore, cell functional experiments were conducted to verify the biological behavior of SEC61G in lung adenocarcinoma cells (LAC). Results SEC61G was upregulated in pan-cancers, including LUAD. High SEC61G expression was significantly correlated with worse prognosis in LUAD patients. Multivariate analysis demonstrated that high SEC61G expression was an independent prognostic factor in the TCGA cohort. (HR = 1.760 95% CI: 1.297–2.388, p < 0.001). The methylation level of SEC61G negatively correlated with the SEC61G expression (R = − 0.290, p < 0.001), and patients with low SEC61G methylation had worse overall survival. (p = 0.0014). Proliferation-associated terms such as cell cycle and cell division were significantly enriched in GO and KEGG analysis. Vitro experiments demonstrated that knockdown of SEC61G resulted in decreased cell proliferation, invasion and facilitated apoptosis in LAC. GSEA analysis found that SEC61G expression was associated with the E2F targets. Moreover, SEC61G expression was negatively correlated with the immune cell infiltration including CD4+ T cell, CD8+ T cell, B cell, macrophage, neutrophil, and dendritic cell. Conclusion Our study indicated that overexpression of SEC61G was significantly associated with poor prognosis of LUAD patients and the malignant phenotypes of LUAD cells, suggesting that it could be a novel prognostic biomarker and potential therapeutic target of LUAD.https://doi.org/10.1186/s12885-021-08957-4SEC61GLung adenocarcinomaNovel biomarkersPrognosisProliferationInvasion |
| spellingShingle | Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation BMC Cancer SEC61G Lung adenocarcinoma Novel biomarkers Prognosis Proliferation Invasion |
| title | Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation |
| title_full | Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation |
| title_fullStr | Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation |
| title_full_unstemmed | Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation |
| title_short | Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation |
| title_sort | prognostic value of sec61g in lung adenocarcinoma a comprehensive study based on bioinformatics and in vitro validation |
| topic | SEC61G Lung adenocarcinoma Novel biomarkers Prognosis Proliferation Invasion |
| url | https://doi.org/10.1186/s12885-021-08957-4 |
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