Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein
α-Casozepine, a bioactive peptide from milk casein, displays an anxiolytic-like activity in many species. Since its mode of action is still not elucidated, a study was conducted in Swiss mice to investigate c-Fos expression, a marker of neuronal activity, in different brain areas. After an intraperi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461730525X |
| _version_ | 1831687117445529600 |
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| author | Simon Benoit Catherine Chaumontet Jessica Schwarz Céline Cakir-Kiefer Daniel Tomé Laurent Miclo |
| author_facet | Simon Benoit Catherine Chaumontet Jessica Schwarz Céline Cakir-Kiefer Daniel Tomé Laurent Miclo |
| author_sort | Simon Benoit |
| collection | DOAJ |
| description | α-Casozepine, a bioactive peptide from milk casein, displays an anxiolytic-like activity in many species. Since its mode of action is still not elucidated, a study was conducted in Swiss mice to investigate c-Fos expression, a marker of neuronal activity, in different brain areas. After an intraperitoneal injection of α-casozepine (1 mg/kg), animals were placed either in a non-stressful or in an anxiety-inducing situation triggered with a light/dark box. No effect of α-casozepine on c-Fos expression was observed in the non-stressful situation. In the stressful situation, modulation of neuronal activity by α-casozepine was observed in different brain regions compared to that of vehicle. However, while diazepam, a benzodiazepine, modulated neuronal activity the same way in hippocampus, accumbens nucleus and hypothalamus, differences were observed in c-Fos expression in amygdala and prefrontal cortex compared to α-casozepine. These results strengthen the assumption that the anxiolytic mechanisms of α-casozepine differ partly of those of diazepam. |
| first_indexed | 2024-12-20T08:52:41Z |
| format | Article |
| id | doaj.art-fe7c92eba50c40b0a1df4eb85098cf86 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T08:52:41Z |
| publishDate | 2017-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-fe7c92eba50c40b0a1df4eb85098cf862022-12-21T19:46:06ZengElsevierJournal of Functional Foods1756-46462017-11-0138464473Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-caseinSimon Benoit0Catherine Chaumontet1Jessica Schwarz2Céline Cakir-Kiefer3Daniel Tomé4Laurent Miclo5Université de Lorraine, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Équipe «Protéolyse & Biofonctionnalités des Protéines et des Peptides» (PB2P), Vandœuvre-lès-Nancy F-54506, France; INRA, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Unité Sous Contrat 340, Vandœuvre-lès-Nancy F-54506, France; UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, Paris, France; Corresponding author at: UR AFPA, Faculté des Sciences et Technologies, Université de Lorraine, B.P. 70239, 54506 Vandœuvre-lès-Nancy Cedex, France.UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, Paris, FranceIngredia SA, Arras, FranceUniversité de Lorraine, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Équipe «Protéolyse & Biofonctionnalités des Protéines et des Peptides» (PB2P), Vandœuvre-lès-Nancy F-54506, France; INRA, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Unité Sous Contrat 340, Vandœuvre-lès-Nancy F-54506, FranceUMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, Paris, FranceUniversité de Lorraine, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Équipe «Protéolyse & Biofonctionnalités des Protéines et des Peptides» (PB2P), Vandœuvre-lès-Nancy F-54506, France; INRA, Unité de Recherche «Animal et Fonctionnalités des Produits Animaux» (UR AFPA), Unité Sous Contrat 340, Vandœuvre-lès-Nancy F-54506, Franceα-Casozepine, a bioactive peptide from milk casein, displays an anxiolytic-like activity in many species. Since its mode of action is still not elucidated, a study was conducted in Swiss mice to investigate c-Fos expression, a marker of neuronal activity, in different brain areas. After an intraperitoneal injection of α-casozepine (1 mg/kg), animals were placed either in a non-stressful or in an anxiety-inducing situation triggered with a light/dark box. No effect of α-casozepine on c-Fos expression was observed in the non-stressful situation. In the stressful situation, modulation of neuronal activity by α-casozepine was observed in different brain regions compared to that of vehicle. However, while diazepam, a benzodiazepine, modulated neuronal activity the same way in hippocampus, accumbens nucleus and hypothalamus, differences were observed in c-Fos expression in amygdala and prefrontal cortex compared to α-casozepine. These results strengthen the assumption that the anxiolytic mechanisms of α-casozepine differ partly of those of diazepam.http://www.sciencedirect.com/science/article/pii/S175646461730525Xα-CasozepineAnxietyBioactive peptidec-FosNeuronal activity modulation |
| spellingShingle | Simon Benoit Catherine Chaumontet Jessica Schwarz Céline Cakir-Kiefer Daniel Tomé Laurent Miclo Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein Journal of Functional Foods α-Casozepine Anxiety Bioactive peptide c-Fos Neuronal activity modulation |
| title | Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein |
| title_full | Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein |
| title_fullStr | Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein |
| title_full_unstemmed | Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein |
| title_short | Mapping in mice the brain regions involved in the anxiolytic-like properties of α-casozepine, a tryptic peptide derived from bovine αs1-casein |
| title_sort | mapping in mice the brain regions involved in the anxiolytic like properties of α casozepine a tryptic peptide derived from bovine αs1 casein |
| topic | α-Casozepine Anxiety Bioactive peptide c-Fos Neuronal activity modulation |
| url | http://www.sciencedirect.com/science/article/pii/S175646461730525X |
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