Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia
Objectives: The role Staphylococcus aureus antimicrobial resistance genes and toxins play in disease severity, management and outcome in childhood is an emerging field requiring further exploration. Methods: A prospective multisite study of Australian and New Zealand children hospitalised with S. au...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000649 |
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| author | Anita J. Campbell Shakeel Mowlaboccus Geoffrey W. Coombs Denise A. Daley Laila S. Al Yazidi Linny K. Phuong Clare Leung Emma J. Best Rachel H. Webb Lesley Voss Eugene Athan Philip N. Britton Penelope A. Bryant Coen T. Butters Jonathan R. Carapetis Natasha S. Ching Joshua Francis Te-Yu Hung Clare Nourse Samar Ojaimi Alex Tai Nan Vasilunas Brendan McMullan Asha C. Bowen Christopher C. Blyth |
| author_facet | Anita J. Campbell Shakeel Mowlaboccus Geoffrey W. Coombs Denise A. Daley Laila S. Al Yazidi Linny K. Phuong Clare Leung Emma J. Best Rachel H. Webb Lesley Voss Eugene Athan Philip N. Britton Penelope A. Bryant Coen T. Butters Jonathan R. Carapetis Natasha S. Ching Joshua Francis Te-Yu Hung Clare Nourse Samar Ojaimi Alex Tai Nan Vasilunas Brendan McMullan Asha C. Bowen Christopher C. Blyth |
| author_sort | Anita J. Campbell |
| collection | DOAJ |
| description | Objectives: The role Staphylococcus aureus antimicrobial resistance genes and toxins play in disease severity, management and outcome in childhood is an emerging field requiring further exploration. Methods: A prospective multisite study of Australian and New Zealand children hospitalised with S. aureus bacteraemia (SAB) occurred over 24 months (2017–2018). Whole genome sequencing (WGS) data were paired with clinical information from the ISAIAH cohort. Results: 353 SAB isolates were sequenced; 85% methicillin-susceptible S. aureus ([MSSA], 301/353) and 15% methicillin-resistant S. aureus ([MRSA], 52/353). There were 92 sequence types (STs), most commonly ST5 (18%) and ST30 (8%), grouped into 23 clonal complexes (CCs), most frequently CC5 (21%) and CC30 (12%). MSSA comprised the majority of healthcare-associated SAB (87%, 109/125), with principal clones CC15 (48%, 11/21) and CC8 (33%, 7/21). Panton-Valentine leukocidin (PVL)-positive SAB occurred in 22% (76/353); predominantly MSSA (59%, 45/76), community-onset (92%, 70/76) infections. For community-onset SAB, the only microbiological independent predictor of poor outcomes was PVL positivity (aOR 2.6 [CI 1.0–6.2]). Conclusion: From this WGS paediatric SAB data, we demonstrate the previously under-recognized role MSSA has in harbouring genetic virulence and causing healthcare-associated infections. PVL positivity was the only molecular independent predictor of poor outcomes in children. These findings underscore the need for further research to define the potential implications PVL-producing strains may have on approaches to S. aureus clinical management. |
| first_indexed | 2024-04-12T13:53:38Z |
| format | Article |
| id | doaj.art-fe7cbfbe2da84ef0aff55360884b834f |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-12T13:53:38Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-fe7cbfbe2da84ef0aff55360884b834f2022-12-22T03:30:26ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-0129197206Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemiaAnita J. Campbell0Shakeel Mowlaboccus1Geoffrey W. Coombs2Denise A. Daley3Laila S. Al Yazidi4Linny K. Phuong5Clare Leung6Emma J. Best7Rachel H. Webb8Lesley Voss9Eugene Athan10Philip N. Britton11Penelope A. Bryant12Coen T. Butters13Jonathan R. Carapetis14Natasha S. Ching15Joshua Francis16Te-Yu Hung17Clare Nourse18Samar Ojaimi19Alex Tai20Nan Vasilunas21Brendan McMullan22Asha C. Bowen23Christopher C. Blyth24Department of Infectious Diseases, Perth Children's Hospital, Perth, Australia; Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute. Perth, Australia; School of Medicine, University of Western Australia, Perth, Australia; Corresponding author. Mailing address: Perth Children's Hospital, Department of Infectious Diseases, 15 Hospital Ave, Nedlands 6009 Western Australia.College of Science, Health, Engineering and Education, Murdoch University, Murdoch; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Western Australia; School of Biomedical Sciences, University of Western Australia, NedlandsCollege of Science, Health, Engineering and Education, Murdoch University, Murdoch; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Western AustraliaDepartment of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Western Australia; The Australian Group on Antimicrobial Resistance (AGAR)Child Health Department, Sultan Qaboos University Hospital, Muscat, Oman; Department of Immunology and Infectious Diseases, Sydney Children's Hospital, Randwick, Sydney, Australia; The Children's Department of Infectious Diseases and Microbiology, the Children's Hospital at Westmead, NSW, AustraliaDepartment of General Medicine, Infectious Diseases Unit, Royal Children's Hospital, Melbourne, Australia; Infection and Immunity Group, Murdoch Children's Research Institute, Melbourne, AustraliaDepartment of Paediatrics, Wagga Wagga Base Hospital, New South Wales, AustraliaDepartment of Paediatrics; Child and Youth Health, The University of Auckland; The National Immunisation Advisory Centre, The University of Auckland; Department of Infectious Diseases, Starship Children's Hospital, Auckland, New ZealandDepartment of Paediatrics, Child and Youth Health, The University of Auckland; Department of Infectious Diseases Starship Children's Hospital, Auckland, New Zealand; Department of Paediatrics, Kidz First Hospital, Auckland, New ZealandDepartment of Paediatrics, Child and Youth Health, The University of Auckland; Department of Infectious Diseases, Starship Children's Hospital, Auckland, New ZealandDepartment of Infectious Disease, Barwon Health, Geelong, Australia; School of Medicine, Deakin University, Geelong, AustraliaSydney Medical School and Marie Bashir Institute, University of Sydney, NSW, Australia; Department of Infectious Diseases and Microbiology, the Children's Hospital at Westmead, Sydney, AustraliaInfectious Diseases Unit, Department of General Medicine, The Royal Children's Hospital, Parkville, Victoria, Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria, AustraliaDepartment of General Medicine, Infectious Diseases Unit, Royal Children's Hospital, Melbourne, Australia; Infection and Immunity Group, Murdoch Children's Research Institute, Melbourne, AustraliaDepartment of Infectious Diseases, Perth Children's Hospital, Nedlands, Western Australia; Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Western Australia; University of Western Australia. School of Medicine, Perth, Western AustraliaInfection and Immunity, Monash Children's Hospital, Monash Health, Clayton, Victoria, Australia; Department of General Paediatrics, Monash Children's Hospital, Monash Health, Victoria, Australia; Department of Paediatrics, Monash University, Clayton, AustraliaGlobal and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Australia; Department of Paediatrics, Royal Darwin Hospital, Darwin, AustraliaDepartment of Paediatrics, Royal Darwin Hospital, Darwin, AustraliaQueensland Children's Hospital, Brisbane, Australia; Faculty of Medicine, University of Queensland, AustraliaInfection & Immunity, Monash Children's Hospital, Monash Health, Clayton, Victoria, Australia; Department of Paediatrics, Monash University, Clayton, Australia; Monash Infectious Diseases, Monash Health, Clayton, Victoria, AustraliaDepartment of Infectious Disease, Barwon Health, Geelong, AustraliaInfectious Diseases Department, Women's and Children's Hospital, AdelaideDepartment of Immunology and Infectious Diseases, Sydney Children's Hospital, Randwick, Sydney, Australia; School of Women's and Children's Health, University of New South Wales, Sydney, Australia; National Centre for Infections in Cancer, University of Melbourne, Melbourne, AustraliaDepartment of Infectious Diseases, Perth Children's Hospital, Nedlands; Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute; School of Medicine, University of Western Australia, Subiaco; Menzies School of Health Research, Charles Darwin Hospital, Darwin, NTDepartment of Infectious Diseases, Perth Children's Hospital, Nedlands; Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute and School of Medicine, University of Western Australia; Department of Microbiology, PathWest Laboratory Medicine, QEII Medical Centre, Perth, Western AustraliaObjectives: The role Staphylococcus aureus antimicrobial resistance genes and toxins play in disease severity, management and outcome in childhood is an emerging field requiring further exploration. Methods: A prospective multisite study of Australian and New Zealand children hospitalised with S. aureus bacteraemia (SAB) occurred over 24 months (2017–2018). Whole genome sequencing (WGS) data were paired with clinical information from the ISAIAH cohort. Results: 353 SAB isolates were sequenced; 85% methicillin-susceptible S. aureus ([MSSA], 301/353) and 15% methicillin-resistant S. aureus ([MRSA], 52/353). There were 92 sequence types (STs), most commonly ST5 (18%) and ST30 (8%), grouped into 23 clonal complexes (CCs), most frequently CC5 (21%) and CC30 (12%). MSSA comprised the majority of healthcare-associated SAB (87%, 109/125), with principal clones CC15 (48%, 11/21) and CC8 (33%, 7/21). Panton-Valentine leukocidin (PVL)-positive SAB occurred in 22% (76/353); predominantly MSSA (59%, 45/76), community-onset (92%, 70/76) infections. For community-onset SAB, the only microbiological independent predictor of poor outcomes was PVL positivity (aOR 2.6 [CI 1.0–6.2]). Conclusion: From this WGS paediatric SAB data, we demonstrate the previously under-recognized role MSSA has in harbouring genetic virulence and causing healthcare-associated infections. PVL positivity was the only molecular independent predictor of poor outcomes in children. These findings underscore the need for further research to define the potential implications PVL-producing strains may have on approaches to S. aureus clinical management.http://www.sciencedirect.com/science/article/pii/S2213716522000649Staphylococcus aureusPaediatricsMolecularBacteraemiaOutcomesWhole genome sequencing |
| spellingShingle | Anita J. Campbell Shakeel Mowlaboccus Geoffrey W. Coombs Denise A. Daley Laila S. Al Yazidi Linny K. Phuong Clare Leung Emma J. Best Rachel H. Webb Lesley Voss Eugene Athan Philip N. Britton Penelope A. Bryant Coen T. Butters Jonathan R. Carapetis Natasha S. Ching Joshua Francis Te-Yu Hung Clare Nourse Samar Ojaimi Alex Tai Nan Vasilunas Brendan McMullan Asha C. Bowen Christopher C. Blyth Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia Journal of Global Antimicrobial Resistance Staphylococcus aureus Paediatrics Molecular Bacteraemia Outcomes Whole genome sequencing |
| title | Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia |
| title_full | Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia |
| title_fullStr | Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia |
| title_full_unstemmed | Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia |
| title_short | Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia |
| title_sort | whole genome sequencing and molecular epidemiology of paediatric staphylococcus aureus bacteraemia |
| topic | Staphylococcus aureus Paediatrics Molecular Bacteraemia Outcomes Whole genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000649 |
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