Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients
Citrate is widely used as an anticoagulant for platelet function tests (PFTs). Due to an intrinsic inhibitory effect of citrate on platelet function, hirudin is used as an alternative. However, studies comparing the effect of these anticoagulants on rotational thromboelastometry (ROTEM) platelet who...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2023.2229909 |
| _version_ | 1797684109286309888 |
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| author | Wasanthi Wickramasinghe Bhawani Yasassri Alvitigala Thisarika Perera Panduka Karunanayake Saroj Jayasinghe Senaka Rajapakse Praveen Weeratunga Ananda Wijewickrama Roopen Arya Klaus Goerlinger Lallindra Viranjan Gooneratne |
| author_facet | Wasanthi Wickramasinghe Bhawani Yasassri Alvitigala Thisarika Perera Panduka Karunanayake Saroj Jayasinghe Senaka Rajapakse Praveen Weeratunga Ananda Wijewickrama Roopen Arya Klaus Goerlinger Lallindra Viranjan Gooneratne |
| author_sort | Wasanthi Wickramasinghe |
| collection | DOAJ |
| description | Citrate is widely used as an anticoagulant for platelet function tests (PFTs). Due to an intrinsic inhibitory effect of citrate on platelet function, hirudin is used as an alternative. However, studies comparing the effect of these anticoagulants on rotational thromboelastometry (ROTEM) platelet whole blood impedance aggregometry in thrombocytopenic patients are scant. Cross-sectional study was done in 105 patients who entered the critical phase of Dengue hemorrhagic fever with plasma leakage and severe thrombocytopenia (<100 × 109/L). Samples were collected on two consecutive days and considered as a combined data set for analysis, out of which 200 have been included in the data analysis. Platelet count was used from routine full blood count. ROTEM platelet used TRAPTEM assay, which was performed with 3.2% sodium citrate and 525 ATU/ml hirudin anticoagulated blood. Means of all the TRAPTEM parameters were significantly higher in hirudin, compared to citrate samples (p < .05). Significantly higher overall platelet aggregation was observed in hirudinized samples with a significant mean difference (p < .05) compared to citrate in each quartile of platelet count. Higher platelet aggregation was observed with hirudin compared to citrate in ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients elaborating the importance of using hirudin anticoagulation in PFTs, particularly in patients with severe thrombocytopenia. |
| first_indexed | 2024-03-12T00:25:47Z |
| format | Article |
| id | doaj.art-fe7de8dfcae34b4a961a5930c4a6b354 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:25:47Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-fe7de8dfcae34b4a961a5930c4a6b3542023-09-15T10:38:12ZengTaylor & Francis GroupPlatelets0953-71041369-16352023-12-0134110.1080/09537104.2023.22299092229909Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patientsWasanthi Wickramasinghe0Bhawani Yasassri Alvitigala1Thisarika Perera2Panduka Karunanayake3Saroj Jayasinghe4Senaka Rajapakse5Praveen Weeratunga6Ananda Wijewickrama7Roopen Arya8Klaus Goerlinger9Lallindra Viranjan Gooneratne10University of ColomboUniversity of ColomboUniversity of ColomboUniversity of ColomboUniversity of ColomboUniversity of ColomboUniversity of ColomboNational Institute of Infectious DiseasesKing’s College HospitalUniversity Hospital Essen, University Duisburg-EssenUniversity of ColomboCitrate is widely used as an anticoagulant for platelet function tests (PFTs). Due to an intrinsic inhibitory effect of citrate on platelet function, hirudin is used as an alternative. However, studies comparing the effect of these anticoagulants on rotational thromboelastometry (ROTEM) platelet whole blood impedance aggregometry in thrombocytopenic patients are scant. Cross-sectional study was done in 105 patients who entered the critical phase of Dengue hemorrhagic fever with plasma leakage and severe thrombocytopenia (<100 × 109/L). Samples were collected on two consecutive days and considered as a combined data set for analysis, out of which 200 have been included in the data analysis. Platelet count was used from routine full blood count. ROTEM platelet used TRAPTEM assay, which was performed with 3.2% sodium citrate and 525 ATU/ml hirudin anticoagulated blood. Means of all the TRAPTEM parameters were significantly higher in hirudin, compared to citrate samples (p < .05). Significantly higher overall platelet aggregation was observed in hirudinized samples with a significant mean difference (p < .05) compared to citrate in each quartile of platelet count. Higher platelet aggregation was observed with hirudin compared to citrate in ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients elaborating the importance of using hirudin anticoagulation in PFTs, particularly in patients with severe thrombocytopenia.http://dx.doi.org/10.1080/09537104.2023.2229909citratehirudinplatelet aggregationthrombocytopeniawhole blood impedance aggregometry |
| spellingShingle | Wasanthi Wickramasinghe Bhawani Yasassri Alvitigala Thisarika Perera Panduka Karunanayake Saroj Jayasinghe Senaka Rajapakse Praveen Weeratunga Ananda Wijewickrama Roopen Arya Klaus Goerlinger Lallindra Viranjan Gooneratne Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients Platelets citrate hirudin platelet aggregation thrombocytopenia whole blood impedance aggregometry |
| title | Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients |
| title_full | Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients |
| title_fullStr | Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients |
| title_full_unstemmed | Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients |
| title_short | Hirudin versus citrate as an anticoagulant for ROTEM platelet whole blood impedance aggregometry in thrombocytopenic patients |
| title_sort | hirudin versus citrate as an anticoagulant for rotem platelet whole blood impedance aggregometry in thrombocytopenic patients |
| topic | citrate hirudin platelet aggregation thrombocytopenia whole blood impedance aggregometry |
| url | http://dx.doi.org/10.1080/09537104.2023.2229909 |
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