Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats
Doxorubicin-induced organ toxicities, through inflammation and oxidative stress, reduce its widespread use in the treatment of cancer. Thus, there is need for the development of less toxic and more effective plant-derived drugs and spice supplements to mitigate inflammation and oxidative stress indu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
|
| Series: | Scientific African |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S246822762200151X |
| _version_ | 1797996676156227584 |
|---|---|
| author | Oluseyi A. Akinloye Lateef A. Sulaimon Abiodun O. Adewale Toriola Mubaraq Olanrewaju Salami Odubiyi Abiola |
| author_facet | Oluseyi A. Akinloye Lateef A. Sulaimon Abiodun O. Adewale Toriola Mubaraq Olanrewaju Salami Odubiyi Abiola |
| author_sort | Oluseyi A. Akinloye |
| collection | DOAJ |
| description | Doxorubicin-induced organ toxicities, through inflammation and oxidative stress, reduce its widespread use in the treatment of cancer. Thus, there is need for the development of less toxic and more effective plant-derived drugs and spice supplements to mitigate inflammation and oxidative stress induced by doxorubicin. The present study investigated the phyto-chemical profile and ameliorative potential of Allium vineale bulb methanol extract (AVBME) on doxorubicin-induced oxidative stress and inflammation in Sprague Dawley rats. Forty-nine (49) male Sprague Dawley rats were randomly stratified into 7 groups with 7 rats per group. Groups A and B received distilled water for 7 days. Groups C, D, and E were pretreated for 7 days with 200 mg/kg silymarin, 500 mg/kg, and 1000 mg/kg AVBME, respectively followed by intraperitoneal injection of 20 mg/kg doxorubicin (DOX) to groups B, C, D, and E on the 8th day. Groups F and G were orally administered 500 mg/kg and 1000 mg/kg AVBME respectively for 7 days with an intraperitoneal injection of distilled water on the 8th day. The AVBME GC–MS analysis revealed the presence of 21 phyto-compounds, rich in flavonoids and LD50 ˃ 2000 mg/kg. AVBME exhibited a very strong inhibitory activity against DPPH, ABTS+, OH., NO, and H2O2 with IC50 values of 0.09, 0.01, 0.036, 2.09, and 3.57 mg/ml respectively. Pre-treatment with AVBME and Silymarin for 7 days increased the PCV, RBC and lymphocytes with the highest WBC count recorded in DOX-administered rats compared to the healthy control. Also, pre-treatment with AVBME for 7 days significantly (p < 0.05) decreased the levels of AST, ALT and ALP in the serum while improving the levels of urea and electrolytes to near basal level in the serum and kidneys. However, doxorubicin administered on the 8th day significantly (p < 0.05) reduced the levels of GSH and antioxidant enzymes in the serum, liver, heart and kidneys while the levels of TNF-α and C-reactive protein increased. Interestingly, pre-treatment with AVBME for 7 days elevated IL-10 and reversed all the alterations induced by doxorubicin toxicity. Also, doxorubicin induced degenerative hepatic, nephrotic and cardiac biochemical and histological changes, while remarkable reversal of these pathological features was observed in groups pretreated with AVBME. AVBME protects the liver, heart and kidneys of Sprague Dawley rats through antioxidative and anti-inflammatory effects. |
| first_indexed | 2024-04-11T10:21:09Z |
| format | Article |
| id | doaj.art-fe804d19c004485282398d55003ad46b |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-04-11T10:21:09Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-fe804d19c004485282398d55003ad46b2022-12-22T04:29:46ZengElsevierScientific African2468-22762022-07-0116e01244Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley RatsOluseyi A. Akinloye0Lateef A. Sulaimon1Abiodun O. Adewale2Toriola Mubaraq3Olanrewaju Salami4Odubiyi Abiola5Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, NigeriaDepartment of Chemical Sciences, College of Natural and Applied Science, Crescent University, Abeokuta, Ogun State, Nigeria; Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria; Corresponding author.Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, NigeriaDoxorubicin-induced organ toxicities, through inflammation and oxidative stress, reduce its widespread use in the treatment of cancer. Thus, there is need for the development of less toxic and more effective plant-derived drugs and spice supplements to mitigate inflammation and oxidative stress induced by doxorubicin. The present study investigated the phyto-chemical profile and ameliorative potential of Allium vineale bulb methanol extract (AVBME) on doxorubicin-induced oxidative stress and inflammation in Sprague Dawley rats. Forty-nine (49) male Sprague Dawley rats were randomly stratified into 7 groups with 7 rats per group. Groups A and B received distilled water for 7 days. Groups C, D, and E were pretreated for 7 days with 200 mg/kg silymarin, 500 mg/kg, and 1000 mg/kg AVBME, respectively followed by intraperitoneal injection of 20 mg/kg doxorubicin (DOX) to groups B, C, D, and E on the 8th day. Groups F and G were orally administered 500 mg/kg and 1000 mg/kg AVBME respectively for 7 days with an intraperitoneal injection of distilled water on the 8th day. The AVBME GC–MS analysis revealed the presence of 21 phyto-compounds, rich in flavonoids and LD50 ˃ 2000 mg/kg. AVBME exhibited a very strong inhibitory activity against DPPH, ABTS+, OH., NO, and H2O2 with IC50 values of 0.09, 0.01, 0.036, 2.09, and 3.57 mg/ml respectively. Pre-treatment with AVBME and Silymarin for 7 days increased the PCV, RBC and lymphocytes with the highest WBC count recorded in DOX-administered rats compared to the healthy control. Also, pre-treatment with AVBME for 7 days significantly (p < 0.05) decreased the levels of AST, ALT and ALP in the serum while improving the levels of urea and electrolytes to near basal level in the serum and kidneys. However, doxorubicin administered on the 8th day significantly (p < 0.05) reduced the levels of GSH and antioxidant enzymes in the serum, liver, heart and kidneys while the levels of TNF-α and C-reactive protein increased. Interestingly, pre-treatment with AVBME for 7 days elevated IL-10 and reversed all the alterations induced by doxorubicin toxicity. Also, doxorubicin induced degenerative hepatic, nephrotic and cardiac biochemical and histological changes, while remarkable reversal of these pathological features was observed in groups pretreated with AVBME. AVBME protects the liver, heart and kidneys of Sprague Dawley rats through antioxidative and anti-inflammatory effects.http://www.sciencedirect.com/science/article/pii/S246822762200151XAllium vinealeOxidative stressInflammationDoxorubicinSprague Dawley rats |
| spellingShingle | Oluseyi A. Akinloye Lateef A. Sulaimon Abiodun O. Adewale Toriola Mubaraq Olanrewaju Salami Odubiyi Abiola Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats Scientific African Allium vineale Oxidative stress Inflammation Doxorubicin Sprague Dawley rats |
| title | Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats |
| title_full | Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats |
| title_fullStr | Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats |
| title_full_unstemmed | Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats |
| title_short | Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats |
| title_sort | allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in sprague dawley rats |
| topic | Allium vineale Oxidative stress Inflammation Doxorubicin Sprague Dawley rats |
| url | http://www.sciencedirect.com/science/article/pii/S246822762200151X |
| work_keys_str_mv | AT oluseyiaakinloye alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats AT lateefasulaimon alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats AT abiodunoadewale alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats AT toriolamubaraq alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats AT olanrewajusalami alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats AT odubiyiabiola alliumvinealemethanolextractattenuatedoxidativestressandinflammationinducedbydoxorubicininspraguedawleyrats |